Disease relevance of EINECS 224-583-8

• Hemolysis of human erythrocytes induced by free radicals initiated from lipid-soluble 2,2'-azobis(2,4-dimethylvaleronitrile) (ADVN) was examined under various conditions [1].

High impact information on EINECS 224-583-8

• The ability of DT-diaphorase to maintain the reduced state of CoQ and protect membrane components from free radical damage as lipid peroxidation was tested by incorporating either reduced CoQ9 or CoQ10 and the lipophylic azoinitiator 2,2'-azobis(2,4-dimethylvaleronitrile) into multilamellar vesicles in the presence of NADH and DT-diaphorase [2].
• TMG acted as an effective inhibitor on lipid peroxidation of egg yolk phosphatidylcholine (PC)-liposomal suspension induced by a water-soluble and a lipid-soluble radical generator, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) [3].
• MeO-AMVN decomposed about 15 times as fast as 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN), a widely used lipophilic azo initiator, and MeO-AMVN-initiated free radical-mediated peroxidations of lipids in organic solution and in micelles, membranes, and low-density lipoprotein in aqueous dispersions with much smaller concentration than AMVN [4].
• To test this hypothesis, we have undertaken studies to examine the role of AmB in oxidative stress in cultured rat aortic smooth muscle cells (SMC) incubated in the absence or in the presence of a lipid-soluble azo-initiator of peroxyl radicals, 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) [5].
• To confirm the involvement of the phenolic moiety in the sesaminol structure in antioxidant activity, sesaminols were reacted with 2,2'-azobis(2,4-dimethylvaleronitrile) [6].

Biological context of EINECS 224-583-8

• The peroxyl radical initiator 2,2'-azobis(2,4-dimethylvaleronitrile) caused a pronounced loss of all major phospholipid classes in PC12 cells, but no loss of cell viability [7].
• Mock-transfected PC12 rat pheochomocytoma cells and PC12 cells transfected with the bcl-2 gene, a gene associated with inhibition of apoptosis, were subjected to oxidative stress by incubation in the presence of the azo-initiator of lipid peroxyl radicals, 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) [8].
• Autoxidation of methyl linoleate (8:2 mixture with decane, 37 degrees C) was induced by 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN, 17.7 mM) and the kinetics of oxygen consumption monitored using a 96-well microplate coated with an oxygen-sensitive fluorescence probe, a ruthenium dye, embedded in a silicone matrix at the bottom of the microplate [9].

Anatomical context of EINECS 224-583-8

• The hydrophobic 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) radical and H(2)O(2) increased plasma membrane damage, and the hydrophilic 2-2'-azobis(2-amidinopropane) dihydrochloride (AAPH) radical and buthionine sulfoxyimine (BSO) increased oxidation of intracellular substances [10].
• Accordingly, we determined the sensitivity of cultured sheep pulmonary artery endothelial cells (SPAEC) that overexpressed MT to tert-butyl hydroperoxide (t-BOOH), hyperoxia, or 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN; peroxyl radical generator) [11].

Associations of EINECS 224-583-8 with other chemical compounds

• We found that sesaminols, a mixture of sesaminol and its stereoisomers, are potent inhibitors of the oxidation of low-density lipoprotein induced by 2,2'-azobis(2,4-dimethylvaleronitrile) [6].
• Lipid peroxidation by the lipophilic free radical generator 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) was little inhibited by uric acid, but not by alpha-tocopherol [12].

References