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Chemical Compound Review

Tris chloride     2-amino-2- (hydroxymethyl)propane-1,3- diol...

Synonyms: TRIS NIEaNI, TRIS HCl, T2413_SIGMA, T2538_SIGMA, T2663_SIGMA, ...
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Disease relevance of Trizma


High impact information on Trizma

  • The activity remained at the top of a sucrose gradient, well resolved from the virosome fraction, at the low salt levels (0.01 M KCl, 0.01 M Tris hydrochloride, pH 8.0) normally used in the course of virosome purification [5].
  • Fragment B was separated from fragment A by ion-exchange column chromatography on a Mono Q column equilibrated with 20 mM Tris hydrochloride buffer (pH 7.6), containing 1 mM dithiothreitol and 2 M urea, in a fast-protein liquid chromatography system by elution with a linear gradient of 0 to 0.5 M NaCl [6].
  • The detection system consisted of 0.25% particulate elastin suspended in a pH 7.0, 0.05 M tris(hydroxymethyl)aminomethane-hydrochloride-buffered solidified plated medium [7].
  • Bilateral superficial femoral arteries were deendothelialized with a 2F arterial embolectomy catheter. rTM (145 microg/kg; 2.0 microg/mL in circulation) or Tris-hydrochloride vehicle control was administered intravenously during the procedure, then either discontinued (group A) or administered twice daily for an additional 48 hours (group B) [8].
  • The purified enzyme showed its maximal activity at 60 degrees C (Ea = 5.1 kcal/mol [21.3 kJ/mol]) and optimal pH values of 8.0 and 7.5 when assayed in Tris hydrochloride and potassium phosphate buffers, respectively [9].

Anatomical context of Trizma

  • The mechanism of induction of competence for DNA uptake in connection with removal of the outer two protein layers of the cell wall by treatment of B. brevis 47 cells with Tris-hydrochloride buffer is discussed [10].
  • Virus pellets were resuspended in Tris-hydrochloride buffer containing 3 units of phospholipase-C (type-1) enzyme/ml and incubated for 2 hours at 37 C. All IBV strains tested demonstrated positive HA activity with chicken red blood cells [11].
  • Isolated mitochondria resuspended in tris chloride or potassium phosphate buffer also aggregated, which resulted in a marked decrease in assayable mitochondrial enzyme activity [12].
  • 2. Heart mitochondria frozen and thawed repeatedly in a sucrose/Tris-chloride buffer retained both their functional integrity as measured by the respiratory control ratio and their ability to serve as an antigen in a complement fixation test [13].

Associations of Trizma with other chemical compounds


Analytical, diagnostic and therapeutic context of Trizma

  • A preparative procedure for red cell actin is based on the dissociation of the membrane skeletal complex in a buffer containing 1 M Tris hydrochloride, followed by gel filtration chromatography in the same medium [18].


  1. Purification and properties of a cell-bound bacteriocin from a Bacteroides fragilis strain. Southern, J.A., Katz, W., Woods, D.R. Antimicrob. Agents Chemother. (1984) [Pubmed]
  2. Method for the lysis of Gram-positive, asporogenous bacteria with lysozyme. Chassy, B.M., Giuffrida, A. Appl. Environ. Microbiol. (1980) [Pubmed]
  3. Heat-induced blebbing and vesiculation of the outer membrane of Escherichia coli. Katsui, N., Tsuchido, T., Hiramatsu, R., Fujikawa, S., Takano, M., Shibasaki, I. J. Bacteriol. (1982) [Pubmed]
  4. Autolysis of Listeria monocytogenes. Popowska, M., Kłoszewska, M., Górecka, S., Markiewicz, Z. Acta Microbiol. Pol. (1999) [Pubmed]
  5. Type I topoisomerase activity after infection of enucleated, synchronized mouse L cells by vaccinia virus. Poddar, S.K., Bauer, W.R. J. Virol. (1986) [Pubmed]
  6. Isolation, purification, and characterization of fragment B, the NH2-terminal half of the heavy chain of tetanus toxin. Matsuda, M., Lei, D.L., Sugimoto, N., Ozutsumi, K., Okabe, T. Infect. Immun. (1989) [Pubmed]
  7. Production and detection of staphylococcal elastase. Hartman, D.P., Murphy, R.A. Infect. Immun. (1977) [Pubmed]
  8. Recombinant human thrombomodulin inhibits arterial neointimal hyperplasia after balloon injury. Li, J.M., Singh, M.J., Itani, M., Vasiliu, C., Hendricks, G., Baker, S.P., Hale, J.E., Rohrer, M.J., Cutler, B.S., Nelson, P.R. J. Vasc. Surg. (2004) [Pubmed]
  9. Induction, isolation, and some properties of the NADPH-dependent glutamate dehydrogenase from the nonheterocystous cyanobacterium Phormidium laminosum. Martinez-Bilbao, M., Martinez, A., Urkijo, I., Llama, M.J., Serra, J.L. J. Bacteriol. (1988) [Pubmed]
  10. Genetic transformation of Bacillus brevis 47, a protein-secreting bacterium, by plasmid DNA. Takahashi, W., Yamagata, H., Yamaguchi, K., Tsukagoshi, N., Udaka, S. J. Bacteriol. (1983) [Pubmed]
  11. Preparing hemagglutinating antigen from isolates of infectious bronchitis virus. Lashgari, M.S., Newman, J.A. Avian Dis. (1982) [Pubmed]
  12. Buffer-induced rat liver mitochondrial aggregation during differential centrifugation. Petersons, M., Allred, J.B. Physiol. Chem. Phys. (1981) [Pubmed]
  13. Characterization of autoantigenic sites on isolated dog heart mitochondria. Kelley, R.E., Olson, M.S., Pinckard, R.N. Biochim. Biophys. Acta (1975) [Pubmed]
  14. Pathologic concentrations of ammonium ions block L-glutamate uptake. Mena, E.E., Cotman, C.W. Exp. Neurol. (1985) [Pubmed]
  15. Hypochlorite injury of Clostridium botulinum spores alters germination responses. Foegeding, P.M., Busta, F.F. Appl. Environ. Microbiol. (1983) [Pubmed]
  16. Reduced glutathione inhibits rabbit and rat skeletal muscle lactate dehydrogenase and prevents dinitrophenol induced extracellular acidification by an epithelial cell line. Wimberger, P., Ebner, S., Marin-Grez, M. Life Sci. (1997) [Pubmed]
  17. Amplified fragment length polymorphism analysis of the VNTR locus D1S80 in central Spain. Alonso, A., Martin, P., Albarran, C., Sancho, M. Int. J. Legal Med. (1993) [Pubmed]
  18. Concentrated Tris solutions for the preparation, depolymerization, and assay of actin: application to erythroid actin. Pinder, J.C., Sleep, J.A., Bennett, P.M., Gratzer, W.B. Anal. Biochem. (1995) [Pubmed]
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