Disease relevance of Complement Fixation Tests

• This test was applied to 46 sera from individuals with various clinical types of brucellosis, and the results were compared with the results of conventional direct and indirect agglutination and complement-fixation tests [1].
• Although all of the eight acute sera were negative by the complement fixation test, four of them were positive by RIPA, indicating a previous infection by rotavirus [2].
• Sample sera taken from any of the animals immunized with Rev 1 were seropositive in both Rose Bengal and the Complement Fixation tests (RBT, CFT) used for the diagnosis of smooth Brucella infections [3].
• The relationship between HLA B27 anterior uveitis (AU) and evidence of chlamydia trachomatis infection was investigated in 35 consecutive patients attending a uveitis research clinic, using a complement fixation test and lymphocyte transformation test to chlamydia (ornithosis-psittacosis) group antigen [4].
• Mycoplasma infection was diagnosed by repeated antibody determination (complement fixation test) and confirmed using the polymerase chain reaction to detect the pathogen from a tracheal aspirate [5].

High impact information on Complement Fixation Tests

• Complement fixation tests with purified RLA antigens demonstrated antibody titer rises in seven children with RLA in their stools and in two mothers (one symptomatic) from whom acute and convalescent sera were available [6].
• Tumors contained a T-antigen that reacted with BKV-specific T-antibody in immunofluorescence and complement-fixation tests [7].
• When BHK cells infected with HVJ-PB were incubated at 38 C, production of infectious virus, hemagglutinin, and neuraminidase was markedly restrained, whereas a considerable amount of viral nucleocapisid and envelope antigens was detected in the cells by complement fixation tests [8].
• Antibodies to the two components were identified separately in serially diluted samples both by radioimmune precipitation assays and by complement fixation tests employing purified nucleoprotein and M components as antigens [9].
• The complement fixation test suggested the presence of a nonspecific weak antibody activity in the blood of retinitis pigmentosa patients as well as of controls [10].

Chemical compound and disease context of Complement Fixation Tests

• Forty-four dams were positive by rivanol and complement-fixation tests and Brucella abortus field strain was isolated from 14 [11].
• The veronal buffered saline used in the complement fixation test offered a valid alternative to the more complex diluents incorporating gelatin currently used in rubella haemagglutinin assays and haemagglutination inhibition tests [12].
• Four hundred and ninety two samples of serum from blood donors were screened for the presence of antibodies specific to cytomegalovirus using radioimmunoassay, a modified complement fixation test, and five commercially available tests: the Cetus CMV IHA, Abbott CMV total AB EIA, Cytomegalisa Stat EIA, Enzygnost EIA, and Virenz G-CMV EIA [13].
• We also examined a latex agglutination test (PylorisetR Dry; Orion Diagnostica, Espoo, Finland) and a complement fixation test (CFT H. pylori; Institute Virion, Rueschlikon, Switzerland) which have been evaluated by others previously [14].
• Indirect complement fixation test with foot-and-mouth disease virus antigen concentrated by polyethylene glycol precipitation [15].

Biological context of Complement Fixation Tests

• With erythrocyte-sensitizing substance coated on latex there was a decrease in titer when tested by the latex, indirect hemagglutination, and complement fixation tests but none when tested by the microimmunofluorescence test [16].
• Some advantages of the RIA over the complement fixation test were that RIA was less prone to cross-reactions and gave better quantitation of low-titered sera [17].
• Although there was an overall correlation between ELISA for anti-RS IgG antibody in sera, the complement fixation test (r = 0.75, P less than 0.001), and MIF test (r = 0.82, P less than 0.001), the sensitivity of ELISA for antibody responses in convalescent sera of infants from 3 months to 2 years was poor [18].

Anatomical context of Complement Fixation Tests

• Direct immunofluorescence showed linear C3 staining in the basement membrane zone and the complement fixation test demonstrated circulating antibasement membrane zone antibodies at a low titer (1:80) [19].
• The complement fixation test utilizing the microtiter system was applied to study sera from patients with a variety of connective tissue diseases for the presence of antibodies to soluble nuclear antigens [20].

Associations of Complement Fixation Tests with chemical compounds

• Antigen preparations CM, CS and the chromatographeal fractions F1, F2 and F3 were assayed in a complement-fixation test against brood-reacting antisera capable of detecting virus envelope antigen and gs antigen and against syngeneic antisera [21].
• Ribavirin treatment was associated with significantly fewer fourfold or greater rises in antibody to influenza A viral antigen by the complement-fixation test, while rises in hemagglutination-inhibiting antibody titers occurred with equal frequency in both groups [22].
• Of the ewes and goats with a vaccination history, one ewe, expectedly a negative responder, had reactions in the MAT, the complement fixation test, and the rose bengal test but not in the SAT, suggesting a lower sensitivity of the SAT in this case [23].
• The FIAX was compared with the standard tube agglutination, Rivanol precipitation, and complement fixation tests, using 285 serum samples from vaccinated, challenged, or control cattle [24].
• Comparative evaluation of commercial Premier EIA and microimmunodiffusion and complement fixation tests for Coccidioides immitis antibodies [25].

Gene context of Complement Fixation Tests

• When the G protein is taken as antigen, the complement fixation test can be used for the assay of virus-neutralizing antibodies [26].
• Complement fixation tests demonstrate that antiserum to bovine DBH has a 25,000-fold lower immunoreactivity with rat DBH than with bovine DBH [27].
• Enzyme-linked immunosorbent assay with CSP antigen under optimal conditions was from 100- to 700-fold more sensitive than the standard agglutination, card, Rivanol precipitation-plate agglutination, and the complement fixation tests in detecting immunoglobulin G antibody [28].
• High IgG titres were noted in most (88-96%) of the patients by MOMP EIA and EIA kit, and in fewer patients (50%) by Re LPS EIA or complement fixation test [29].
• Comparison between the COA Test and complement fixation test for the detection and typing of FMDV obtained from extracts of tongue epithelial tissues from infected cattle revealed high agreement in the results and indicated a potential application of the COA Test for the direct diagnosis of viruses [30].

Analytical, diagnostic and therapeutic context of Complement Fixation Tests

• Sera of these patients were retested for anti-M2, anti-M4, anti-M8 and anti-M9 antibodies by ELISA and the complement fixation test (CFT) [31].
• The complement fixation test using histoplasmin as antigen detected antibodies in the sera of 72.8% of the 70 proven cases, while the complement fixation test using yeast antigen detected antibodies in 94.3% of the case sera, and the immunodiffusion test using histoplasmin antigen detected antibodies in 90% of the sera [32].
• Enzyme immunoassays with either the CMV glycoproteins or the whole CMV antigen from infected cell lysates were superior to a complement fixation test with a glycine extract antigen for serodiagnosis of current infection [33].
• The nonspecific cytoplasmic staining that occurs in indirect immunofluorescence tests for CMV did not occur in the ACIF system, and sera that were anti-complementary in complement fixation tests could be examined satisfactorily by ACIF [34].
• For periods of up to 1 year after infection, 172 sera were obtained and tested by complement fixation test (CFT), fluorescent assays for titrating specific IgG, IgA, and IgM, and Western blotting [35].

References