Disease relevance of Blmh

• Compared with controls, Blmh null mice were more sensitive to acute BLM lethality and developed pulmonary fibrosis more readily following BLM treatment [1].
• Mice lacking Blmh exhibited variably penetrant tail dermatitis that resembled rodent ringtail [1].
• The antibody significantly reacted with bleomycin hydrolase from rabbit tissues, mouse livers, sarcoma 180, and adenocarcinoma 755 but not significantly with that from MH 134 and Ehrlich carcinoma [2].
• Potentiation of the cytotoxicity of peplomycin against Ehrlich ascites carcinoma by bleomycin hydrolase inhibitors [3].
• Metastasizing fibrosarcomas in hamsters (BMH) after injection of B77 sarcoma virus transformed mouse cells [4].

High impact information on Blmh

• Bleomycin (BLM), a clinically used glycopeptide anticancer agent, is deaminated in vitro by Blmh [1].
• We used gene targeting to generate mice that lack Blmh and demonstrated that Blmh is the sole enzyme required for BLM deamination [1].
• The papain superfamily member bleomycin hydrolase (Blmh) is a neutral cysteine protease with structural similarity to a 20S proteasome [1].
• The neutral cysteine protease bleomycin hydrolase is essential for epidermal integrity and bleomycin resistance [1].
• Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing on a polyacrylamide slab gel of purified bleomycin hydrolase showed a single band with an apparent Mr of 48K and an isoelectric pH of 5 [2].

Chemical compound and disease context of Blmh

• A comparative study on the endotoxic effects of lipopolysaccharide (LPS) from Veillonella parvula ATCC 10790 and from Bacteroides intermedius BMH was performed using an in vivo approach in the C57BL/6 mouse [5].

Anatomical context of Blmh

• BMH tumor cells injected into syngeneic newborn as well as young adult hamsters produced tumors at the site of application and metastasized frequently into viscera [4].
• From hamster tumors five stable tumor cell lines (BMH/1--BMH/5) were established in vitro [4].

Analytical, diagnostic and therapeutic context of Blmh

• Bleomycin hydrolase was purified from rabbit liver by immunoaffinity with the monoclonal antibody and DEAE gel chromatography [2].

References