Disease relevance of PLEKHC1

• These findings suggest that mig-2 expression is transcriptionally elevated in leiomyomas and could be involved in its hormone-mediated growth of leiomyomas of the uterus [1].
• In contrast, the mig-2 expression was greatly decreased in leiomyosarcomas [1].
• Examination of 30 frozen breast tumors supported the finding that MIG2 is overexpressed in a subset of breast cancers [2].

High impact information on PLEKHC1

• Loss of Mig-2 or migfilin impairs cell shape modulation [3].
• Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation [3].
• The UNC-112 gene in Caenorhabditis elegans encodes a novel component of cell-matrix adhesion structures required for integrin localization in the muscle cell membrane [4].
• In situ hybridizations showed that Kindlin-1 is preferentially expressed in epithelia, and Kindlin-2 in striated and smooth muscle cells [5].
• While both localize to integrin-mediated adhesion sites in cultured keratinocytes Kindlin-2, but not Kindlin-1, colocalizes with E-cadherin to cell-cell contacts in differentiated keratinocytes [5].

Chemical compound and disease context of PLEKHC1

• Although all 21 normal tissues from reduction mammoplasty showed immunoreactivity for MIG2, ranging from weak (62%) to strong (24%), only half of the 34 formalin-fixed breast tumors showed immunoreactivity for MIG2 [2].

Biological context of PLEKHC1

• Due to the strong sequence homology to the C. elegans UNC-112 we call this gene URP1, for UNC-112 related protein [6].
• Northern blotting, Western blotting, and immunohistochemical staining demonstrated that mig-2 expression was increased in leiomyomas compared with normal myometrium, especially during the secretory phase of the menstrual cycle [1].

Anatomical context of PLEKHC1

• In this study, we investigated the role of ILK, and its binding partners PINCH, alpha-parvin, and Mig-2 in matrix-induced hepatocyte differentiation [7].
• Using real-time reverse transcription-PCR, we found that mitogen-inducible gene 2 (MIG2) is expressed at a 17-fold higher level in TMX2-28 cells than in nonaggressive MCF-7 cells and that MIG2 mRNA levels are low in the nontumorigenic human mammary epithelial cell line, 184 [2].

Other interactions of PLEKHC1

• We have also isolated the full-length clones for another novel related gene, URP2 and the previously discovered MIG-2 gene [6].
• We also show that ILK, PINCH, alpha-parvin, and Mig-2 expression level is dramatically reduced in the re-differentiated hepatocytes [7].

Analytical, diagnostic and therapeutic context of PLEKHC1

• Direct sequencing of the whole coding region of mig-2 cDNA and Southern blotting revealed that mig-2 alterations, such as mutations, rearrangement, and amplification, were not present in either leiomyomas or leiomyosarcomas [1].
• Finally, MIG2 expression was evaluated in reductive mammoplasty and breast tumor tissue [2].

References