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Gene Review

Eif4g2  -  eukaryotic translation initiation factor 4...

Mus musculus

Synonyms: AA589388, DAP-5, E130105L11Rik, Eukaryotic translation initiation factor 4 gamma 2, Nat1, ...
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Disease relevance of Eif4g2

  • Their outgrowth in immunocompetent syngeneic mice was shown to inversely correlate with p97 antigen expression, and lines that express higher p97 levels elicited a stronger delayed-type hypersensitivity response when injected into the footpads of mice immune to p97 [1].
  • Mice immunized with v-p97NY rejected transplants of syngeneic mouse melanoma expressing p97 [2].
  • The L49 monoclonal antibody against the p97 antigen on melanomas and carcinomas was chemically conjugated to E. coli beta-galactosidase (beta-gal), forming a largely monomeric conjugate with preserved enzymatic activity [3].

High impact information on Eif4g2

  • Cryoelectron microscopy and single-particle analysis revealed that a major conformational change of p97 during the ATPase cycle occurred upon nucleotide binding and not during hydrolysis as previously hypothesized [4].
  • Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway [5].
  • Binding of the p47 complex directs p97 to act in the post-mitotic fusion of Golgi membranes [6].
  • In rat liver cytosol, Ufd1 and Npl4 form a binary complex, which exists either alone or bound to p97 [6].
  • Because p97 is known to bind to the endoplasmic reticulum membrane protein AMFR (gp78), an E3 ligase, we propose a model in which p97, mPNGase, and mHR23B mediate interaction of the endoplasmic reticulum with the proteasome [7].

Biological context of Eif4g2


Anatomical context of Eif4g2

  • We activated T-lymphocytes in vivo with SEB, expanded them in vitro with IL-2, and directed them against a tumour target with the BAb 500A2 x 96.5, specific for the murine CD3 antigen and the melanoma p97 antigen expressed by the CL62 tumour [11].

Associations of Eif4g2 with chemical compounds


Analytical, diagnostic and therapeutic context of Eif4g2

  • Northern blot analysis shows that Eif4g2 is ubiquitously expressed at high levels in all human and mouse tissues examined [8].
  • Nat1- and Nat2-specific mRNA, determined by quantitative real-time polymerase chain reaction, was detected in all tissues examined and did not differ significantly (p > 0.05) between Nat2 KO and WT mice [13].
  • Western blot analysis of whole cell lysates using anti-phosphotyrosine antibody revealed that GH promotes the tyrosyl phosphorylation of a 121-kDa protein and several other proteins (p97, p42, p39) in a dose- and time-dependent fashion [14].
  • We can show that, in contrast to the nuclear localization signal-dependent transport, the phorbol ester-induced transport of protein kinase C alpha is not affected by microinjection of antibodies against the nuclear import factor p97/importin/karyopherin beta or microinjection of non-hydrolyzable GTP-analogs [10].


  1. Transfected mouse melanoma lines that express various levels of human melanoma-associated antigen p97. Estin, C.D., Stevenson, U., Kahn, M., Hellström, I., Hellström, K.E. J. Natl. Cancer Inst. (1989) [Pubmed]
  2. Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy. Estin, C.D., Stevenson, U.S., Plowman, G.D., Hu, S.L., Sridhar, P., Hellström, I., Brown, J.P., Hellström, K.E. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  3. Generation of an intensely potent anthracycline by a monoclonal antibody-beta-galactosidase conjugate. Torgov, M.Y., Alley, S.C., Cerveny, C.G., Farquhar, D., Senter, P.D. Bioconjug. Chem. (2005) [Pubmed]
  4. A major conformational change in p97 AAA ATPase upon ATP binding. Rouiller, I., Butel, V.M., Latterich, M., Milligan, R.A., Wilson-Kubalek, E.M. Mol. Cell (2000) [Pubmed]
  5. Essential role of NAT1/p97/DAP5 in embryonic differentiation and the retinoic acid pathway. Yamanaka, S., Zhang, X.Y., Maeda, M., Miura, K., Wang, S., Farese, R.V., Iwao, H., Innerarity, T.L. EMBO J. (2000) [Pubmed]
  6. A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways. Meyer, H.H., Shorter, J.G., Seemann, J., Pappin, D., Warren, G. EMBO J. (2000) [Pubmed]
  7. Multiple modes of interaction of the deglycosylation enzyme, mouse peptide N-glycanase, with the proteasome. Li, G., Zhou, X., Zhao, G., Schindelin, H., Lennarz, W.J. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  8. cDNA cloning, expression analysis, and chromosomal localization of a gene with high homology to wheat eIF-(iso)4F and mammalian eIF-4G. Shaughnessy, J.D., Jenkins, N.A., Copeland, N.G. Genomics (1997) [Pubmed]
  9. Cloning and characterization of the murine glucosamine-6-phosphate acetyltransferase EMeg32. Differential expression and intracellular membrane association. Boehmelt, G., Fialka, I., Brothers, G., McGinley, M.D., Patterson, S.D., Mo, R., Hui, C.C., Chung, S., Huber, L.A., Mak, T.W., Iscove, N.N. J. Biol. Chem. (2000) [Pubmed]
  10. Nuclear import of protein kinase C occurs by a mechanism distinct from the mechanism used by proteins with a classical nuclear localization signal. Schmalz, D., Hucho, F., Buchner, K. J. Cell. Sci. (1998) [Pubmed]
  11. The bispecific antibody 500A2 x 96.5 targets T-lymphocytes activated in vivo with staphylococcal enterotoxin B (SEB) against CL62 melanoma cells in vitro. Reid, I.M., Lundy, J.K., Donohue, J.H. Surgical oncology. (1994) [Pubmed]
  12. Angiotensin II stimulates tyrosine phosphorylation of phospholipase C-gamma-associated proteins. Characterization of a c-Src-dependent 97-kD protein in vascular smooth muscle cells. Schmitz, U., Ishida, M., Berk, B.C. Circ. Res. (1997) [Pubmed]
  13. N-acetyltransferase (nat) 1 and 2 expression in nat2 knockout mice. Loehle, J.A., Cornish, V., Wakefield, L., Doll, M.A., Neale, J.R., Zang, Y., Sim, E., Hein, D.W. J. Pharmacol. Exp. Ther. (2006) [Pubmed]
  14. Growth hormone-promoted tyrosyl phosphorylation of a 121-kDa growth hormone receptor-associated protein. Wang, X., Möller, C., Norstedt, G., Carter-Su, C. J. Biol. Chem. (1993) [Pubmed]
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