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Gene Review

EIF4G2  -  eukaryotic translation initiation factor 4...

Homo sapiens

Synonyms: AAG1, DAP-5, DAP5, Death-associated protein 5, Eukaryotic translation initiation factor 4 gamma 2, ...
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Disease relevance of EIF4G2


Psychiatry related information on EIF4G2

  • On whole body images, the anti-Fab HMWA appears to be more tumor selective than Fab preparations that target the p97 antigen for melanoma, and there is less uptake in liver [6].

High impact information on EIF4G2


Chemical compound and disease context of EIF4G2


Biological context of EIF4G2

  • In addition, downregulation of both CEP63 and EIF4G2 gene transcription was associated with invasive tumors [1].
  • While cap-dependent translation from this transfected vector was reduced during Fas-induced apoptosis, the translation via the DAP5 IRES was selectively maintained [11].
  • An internal ribosome entry site (IRES) element capable of directing the translation of a reporter gene when subcloned into a bicistronic vector was identified in the 5' untranslated region of DAP5 mRNA [11].
  • Transient transfection experiments show that p97 suppresses both cap-dependent and independent translation, while eIF4G supports both translation pathways [12].
  • The rescued DAP-5 cDNA fragment which conveyed resistance to IFN-gamma-induced cell death was expressed from the vector in the sense orientation [13].

Anatomical context of EIF4G2


Associations of EIF4G2 with chemical compounds

  • This study reveals why human NAT1 acetylates the sunscreen additive p-aminobenzoic acid and tobacco smoke carcinogen 4-aminobiphenyl, but not o-toluidine and other arylamines linked to bladder cancer [19].
  • We propose a model that assigns the cytosolic face of the ER as a midpoint to which luminal ERAD substrates emerge and p97/Cdc48p and the proteasome are recruited [20].
  • Site-directed mutagenesis of conserved cysteine residues in the pore- and receptor-binding domains identified two cysteines, C223 and C228, that were required for p97 to bind the nuclear pore [17].
  • In a permeabilized cell protein import assay, a mutant p97 with alanine substituted for Cys-158 is unable to support import in the presence of NLS receptor and Ran [21].
  • The N-ethylmaleimide sensitivity of the p97 for docking was investigated and found to be due to inhibition of p97 binding to the pore complex and to the NLS receptor [17].

Regulatory relationships of EIF4G2


Other interactions of EIF4G2

  • The N-terminal part of DAP-5 has 39% identity and 63% similarity to the central region of mammalian p220 [13].
  • Intriguingly, it comprised part of the coding region which corresponds to the less conserved C-terminal part of DAP-5 and directed the synthesis of a 28-kDa miniprotein [13].
  • DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death [13].
  • When the HeLa cell extract was supplemented with a combination of eIF2, eIF2B, and p97, the capacity to synthesize a protein from an uncapped mRNA became comparable to that from the capped counterpart stimulated with a combination of eIF2, eIF2B, and eIF4E [22].
  • In humans, two isoforms have been characterized that are generated by alternative splicing and contain either exon 1a or 1b (hAAG1 or hAAG2) [23].

Analytical, diagnostic and therapeutic context of EIF4G2

  • Among the differentially expressed genes, SFRP1,CEP63 and EIF4G2 were further validated by quantitative RT-PCR in a series of 50 transitional cell carcinomas [1].
  • METHODS: Peripheral blood from 140Han people were collected and analyzed for NAT1 genotypes by allele-specific PCR combining with PCR-based restriction fragment length polymorphism-based procedure [14].
  • The NAT1 phenotype were determined according to the NAT1 enzyme kinetics in leukocytes by HPLC method and the values of intrinsic clearance (Cl(int)) and V(max) and Michaelis constant (K(m)) of NAT1 were calculated [14].
  • Although fibroblasts and lymphocytes express only small amounts of p97, we were able to type the hybrids for p97 by using monoclonal antibodies in highly sensitive and specific immunoassays [4].
  • Localization of 131I-labeled p97-specific Fab fragments in human melanoma as a basis for radiotherapy [24].


  1. The transcripts of SFRP1,CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder. Buim, M.E., Soares, F.A., Sarkis, A.S., Nagai, M.A. Oncology (2005) [Pubmed]
  2. Cleavage of eukaryotic translation initiation factor 4G by exogenously added hybrid proteins containing poliovirus 2Apro in HeLa cells: effects on gene expression. Novoa, I., Carrasco, L. Mol. Cell. Biol. (1999) [Pubmed]
  3. DAP-5 is involved in MycN/IFNgamma-induced apoptosis in human neuroblastoma cells. Wittke, I., Mädge, B., Wiedemeyer, R., Kimchi, A., Schwab, M. Cancer Lett. (2001) [Pubmed]
  4. Assignment of the gene for human melanoma-associated antigen p97 to chromosome 3. Plowman, G.D., Brown, J.P., Enns, C.A., Schröder, J., Nikinmaa, B., Sussman, H.H., Hellström, K.E., Hellström, I. Nature (1983) [Pubmed]
  5. Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy. Estin, C.D., Stevenson, U.S., Plowman, G.D., Hu, S.L., Sridhar, P., Hellström, I., Brown, J.P., Hellström, K.E. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  6. Use of I-131 labeled, murine Fab against a high molecular weight antigen of human melanoma: preliminary experience. Larson, S.M., Carrasquillo, J.A., McGuffin, R.W., Krohn, K.A., Ferens, J.M., Hill, L.D., Beaumier, P.L., Reynolds, J.C., Hellström, K.E., Hellström, I. Radiology. (1985) [Pubmed]
  7. A Role for the Endoplasmic Reticulum Protein Retrotranslocation Machinery during Crosspresentation by Dendritic Cells. Ackerman, A.L., Giodini, A., Cresswell, P. Immunity (2006) [Pubmed]
  8. p97/DAP5 is a ribosome-associated factor that facilitates protein synthesis and cell proliferation by modulating the synthesis of cell cycle proteins. Lee, S.H., McCormick, F. EMBO J. (2006) [Pubmed]
  9. Human monoclonal antibodies produced by primary in vitro immunization of peripheral blood lymphocytes. Borrebaeck, C.A., Danielsson, L., Möller, S.A. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  10. Cyclophosphamide potentiates the antitumor activity of v-p97NY. Estin, C.D., Stevenson, U.S., Hellström, I., Hellström, K.E. Cell. Immunol. (1989) [Pubmed]
  11. A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation. Henis-Korenblit, S., Strumpf, N.L., Goldstaub, D., Kimchi, A. Mol. Cell. Biol. (2000) [Pubmed]
  12. A new translational regulator with homology to eukaryotic translation initiation factor 4G. Imataka, H., Olsen, H.S., Sonenberg, N. EMBO J. (1997) [Pubmed]
  13. DAP-5, a novel homolog of eukaryotic translation initiation factor 4G isolated as a putative modulator of gamma interferon-induced programmed cell death. Levy-Strumpf, N., Deiss, L.P., Berissi, H., Kimchi, A. Mol. Cell. Biol. (1997) [Pubmed]
  14. N-Acetyltransferase-1 gene polymorphisms and correlation between genotype and its activity in a central Chinese Han population. Zhangwei, X., Jianming, X., Qiao, M., Xinhua, X. Clin. Chim. Acta (2006) [Pubmed]
  15. Phosphorylation of a Fes-related protein in response to granulocyte-macrophage colony stimulating factor. Linnekin, D., Mou, S.M., Greer, P., Longo, D.L., Ferris, D.K. J. Biol. Chem. (1995) [Pubmed]
  16. CD4+ T cell clones specific for the human p97 melanoma-associated antigen can eradicate pulmonary metastases from a murine tumor expressing the p97 antigen. Kahn, M., Sugawara, H., McGowan, P., Okuno, K., Nagoya, S., Hellström, K.E., Hellström, I., Greenberg, P. J. Immunol. (1991) [Pubmed]
  17. Functional domains in nuclear import factor p97 for binding the nuclear localization sequence receptor and the nuclear pore. Chi, N.C., Adam, S.A. Mol. Biol. Cell (1997) [Pubmed]
  18. Enhancement of in vitro tumor-infiltrating lymphocyte cytotoxicity by heteroconjugated antibodies. Reid, I., Lundy, J., Donohue, J.H. J. Immunol. (1992) [Pubmed]
  19. NMR-based Model Reveals the Structural Determinants of Mammalian Arylamine N-Acetyltransferase Substrate Specificity. Zhang, N., Liu, L., Liu, F., Wagner, C.R., Hanna, P.E., Walters, K.J. J. Mol. Biol. (2006) [Pubmed]
  20. Distinct steps in dislocation of luminal endoplasmic reticulum-associated degradation substrates: roles of endoplamic reticulum-bound p97/Cdc48p and proteasome. Elkabetz, Y., Shapira, I., Rabinovich, E., Bar-Nun, S. J. Biol. Chem. (2004) [Pubmed]
  21. Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97. Chi, N.C., Adam, E.J., Adam, S.A. J. Biol. Chem. (1997) [Pubmed]
  22. An efficient mammalian cell-free translation system supplemented with translation factors. Mikami, S., Masutani, M., Sonenberg, N., Yokoyama, S., Imataka, H. Protein Expr. Purif. (2006) [Pubmed]
  23. Alkylation resistance of E. coli cells expressing different isoforms of human alkyladenine DNA glycosylase (hAAG). Bonanno, K., Wyrzykowski, J., Chong, W., Matijasevic, Z., Volkert, M.R. DNA Repair (Amst.) (2002) [Pubmed]
  24. Localization of 131I-labeled p97-specific Fab fragments in human melanoma as a basis for radiotherapy. Larson, S.M., Carrasquillo, J.A., Krohn, K.A., Brown, J.P., McGuffin, R.W., Ferens, J.M., Graham, M.M., Hill, L.D., Beaumier, P.L., Hellström, K.E. J. Clin. Invest. (1983) [Pubmed]
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