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Gene Review

Pou4f2  -  POU domain, class 4, transcription factor 2

Mus musculus

Synonyms: Brain-3B, Brain-specific homeobox/POU domain protein 3B, Brn-3.2, Brn-3B, Brn-3b, ...
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Disease relevance of Pou4f2


High impact information on Pou4f2


Biological context of Pou4f2


Anatomical context of Pou4f2


Associations of Pou4f2 with chemical compounds

  • Here we have altered a single isoleucine residue in the POU homeodomain of Brn-3b to the valine residue found at the equivalent position in Brn-3a [12].

Regulatory relationships of Pou4f2

  • Transient co-transfection of a Wt1 expression construct activated a Pou4f2 promoter-reporter construct approximately 4-fold [2].

Other interactions of Pou4f2

  • We find that Brn3b(l) and Brn3b(s), the two isoforms encoded by the Brn3b gene, as well as Brn3a and Brn3c all have similar DNA-binding and transactivating activities [13].
  • In conclusion, we identified Pou4f2 as a novel downstream target gene of Wt1 [2].
  • We found that Brn3b influenced gene expression in non RGCs of the retina by controlling the expression of secreted signaling molecules such as sonic hedgehog and myostatin/Gdf8 [8].
  • Screening with a microarray containing 864 cDNA clones using wild-type and brn-3b (-/-) retinal cDNA probes revealed a potential regulatory linkage between the transcription factor Brn-3b and expression of GAP-43, a protein associated with axon growth [14].

Analytical, diagnostic and therapeutic context of Pou4f2

  • At E14.5, we identified 87 genes whose expression was significantly altered in the absence of Brn3b and verified the results by real-time PCR and in situ hybridization [8].
  • We have used a quantitative RT-PCR approach to determine the levels of Brn3a and Brn3b POU domain transcription factor mRNAs in the developing mouse trigeminal ganglion from E10 to E18 [15].


  1. The Wilms' tumor gene Wt1 is required for normal development of the retina. Wagner, K.D., Wagner, N., Vidal, V.P., Schley, G., Wilhelm, D., Schedl, A., Englert, C., Scholz, H. EMBO J. (2002) [Pubmed]
  2. The Wilms' tumor suppressor Wt1 encodes a transcriptional activator of the class IV POU-domain factor Pou4f2 (Brn-3b). Wagner, K.D., Wagner, N., Schley, G., Theres, H., Scholz, H. Gene (2003) [Pubmed]
  3. Brn-3.2: a Brn-3-related transcription factor with distinctive central nervous system expression and regulation by retinoic acid. Turner, E.E., Jenne, K.J., Rosenfeld, M.G. Neuron (1994) [Pubmed]
  4. Role of transcription factors Brn-3.1 and Brn-3.2 in auditory and visual system development. Erkman, L., McEvilly, R.J., Luo, L., Ryan, A.K., Hooshmand, F., O'Connell, S.M., Keithley, E.M., Rapaport, D.H., Ryan, A.F., Rosenfeld, M.G. Nature (1996) [Pubmed]
  5. Requirement for Brn-3.0 in differentiation and survival of sensory and motor neurons. McEvilly, R.J., Erkman, L., Luo, L., Sawchenko, P.E., Ryan, A.F., Rosenfeld, M.G. Nature (1996) [Pubmed]
  6. A POU domain transcription factor-dependent program regulates axon pathfinding in the vertebrate visual system. Erkman, L., Yates, P.A., McLaughlin, T., McEvilly, R.J., Whisenhunt, T., O'Connell, S.M., Krones, A.I., Kirby, M.A., Rapaport, D.H., Bermingham, J.R., O'Leary, D.D., Rosenfeld, M.G. Neuron (2000) [Pubmed]
  7. Autoregulatory sequences are revealed by complex stability screening of the mouse brn-3.0 locus. Trieu, M., Rhee, J.M., Fedtsova, N., Turner, E.E. J. Neurosci. (1999) [Pubmed]
  8. Discrete gene sets depend on POU domain transcription factor Brn3b/Brn-3.2/POU4f2 for their expression in the mouse embryonic retina. Mu, X., Beremand, P.D., Zhao, S., Pershad, R., Sun, H., Scarpa, A., Liang, S., Thomas, T.L., Klein, W.H. Development (2004) [Pubmed]
  9. POU domain factor Brn-3b is essential for retinal ganglion cell differentiation and survival but not for initial cell fate specification. Gan, L., Wang, S.W., Huang, Z., Klein, W.H. Dev. Biol. (1999) [Pubmed]
  10. Delayed expression of the Crx gene and photoreceptor development in the Chx10-deficient retina. Rutherford, A.D., Dhomen, N., Smith, H.K., Sowden, J.C. Invest. Ophthalmol. Vis. Sci. (2004) [Pubmed]
  11. Brn3b/Brn3c double knockout mice reveal an unsuspected role for Brn3c in retinal ganglion cell axon outgrowth. Wang, S.W., Mu, X., Bowers, W.J., Kim, D.S., Plas, D.J., Crair, M.C., Federoff, H.J., Gan, L., Klein, W.H. Development (2002) [Pubmed]
  12. A single amino acid change converts an inhibitory transcription factor into an activator. Dawson, S.J., Morris, P.J., Latchman, D.S. J. Biol. Chem. (1996) [Pubmed]
  13. All Brn3 genes can promote retinal ganglion cell differentiation in the chick. Liu, W., Khare, S.L., Liang, X., Peters, M.A., Liu, X., Cepko, C.L., Xiang, M. Development (2000) [Pubmed]
  14. Gene expression in the developing mouse retina by EST sequencing and microarray analysis. Mu, X., Zhao, S., Pershad, R., Hsieh, T.F., Scarpa, A., Wang, S.W., White, R.A., Beremand, P.D., Thomas, T.L., Gan, L., Klein, W.H. Nucleic Acids Res. (2001) [Pubmed]
  15. NT-3 regulates expression of Brn3a but not Brn3b in developing mouse trigeminal sensory neurons. Wyatt, S., Ensor, L., Begbie, J., Ernfors, P., Reichardt, L.F., Latchman, D.S. Brain Res. Mol. Brain Res. (1998) [Pubmed]
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