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Dnase1  -  deoxyribonuclease I

Rattus norvegicus

Synonyms: DNase I, Deoxyribonuclease I, Deoxyribonuclease-1, Dnl1
 
 
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Disease relevance of Dnase1

  • We have now used DNase-I hypersensitivity studies to identify a possible binding site for this factor at around -3.6 kilobases (kb) of the TAT gene [1].
 

High impact information on Dnase1

  • We have previously identified a series of five DNase-I hypersensitive (HS) sites within and around the rat phosphoenolpyruvate carboxykinase (PEPCK) gene [2].
  • A putative HNF4alpha-response element in the upstream enhancer of glutamine synthetase (GS), an exclusively pericentral enzyme, was protected against DNase-I and interacted with a protein that is recognized by HNF4alpha-specific antiserum [3].
  • Electrophoretic mobility shift assays and DNase-I protection assays showed that a glucocorticoid response element (GRE) 15-mer located at -1212 bound the glucocorticoid receptor DNA-binding domain protein in a concentration-dependent manner [4].
  • Bandshifting and DNase-I footprinting experiments using this region of the RII beta promoter detected several related specific DNA-protein complexes formed using crude and fractionated nuclear extracts from rat ovary, brain, adrenal gland, and liver [5].
  • To study the mechanism of this tissue-specific and hormone-dependent regulation we have used DNase-I footprinting and band-shift assays to locate binding sites for nuclear proteins isolated from different tissues [6].
 

Chemical compound and disease context of Dnase1

 

Biological context of Dnase1

  • Dnase1 preferentially hydrolysed 'naked' plasmid DNA, whereas Dnase1l3 cleaved nuclear DNA with high activity [8].
  • DNase-I-footprinting analyses indicate that HES-2 binds to all E box sequences (CANNTG) we tested as well as to the N-box sequences (CACNAG) [9].
  • Other well-known sites of apoptotic cell death, including brain of ischemic rat, mammary gland of post-lactating rat and rat intestine as well as Dnase-treated rat brain slices, were used as positive controls [10].
  • The proximal hypersensitive site-1, which is close to the transcription start site in the liver, does coincide with a sequence (designated IRSL) that is 80% identical to the phosphoenolpyruvate carboxykinase IRS and with a DNase-I footprint that has been identified overlapping this sequence [11].
  • The pattern of DNase-I hypersensitivity is similar for the rat endogenous gene and for the complete rat transgene; the liver-specific HSS-1 and HSS-2 are present and the intensity of the sites is correlated to the number of integrated copies [7].
 

Anatomical context of Dnase1

 

Other interactions of Dnase1

  • Previous studies have demonstrated that GH rapidly stimulates IGF-I expression in vivo, and our laboratory has identified a GH-regulated alteration in chromatin configuration, manifested as a hormonally induced deoxyribonuclease-I (DNase-I)-hypersensitive site in the second IGF-I intron [13].
  • When hybridization experiments were conducted, it was found that the DNAs derived from both levels of DNase-I-digested nucleoli were incapable of forming hybrids with the labelled nucleolar RNA synthesized by the engaged RNA polymerase I from the untreated nucleoli [14].

References

  1. Modulation of glucocorticoid induction of stably transfected tyrosine aminotransferase gene constructs involves elements up-stream of the glucocorticoid-responsive element. Szapary, D., Oshima, H., Simons, S.S. Endocrinology (1992) [Pubmed]
  2. Interaction of a liver-specific factor with an enhancer 4.8 kilobases upstream of the phosphoenolpyruvate carboxykinase gene. Ip, Y.T., Poon, D., Stone, D., Granner, D.K., Chalkley, R. Mol. Cell. Biol. (1990) [Pubmed]
  3. Hepatic HNF4alpha deficiency induces periportal expression of glutamine synthetase and other pericentral enzymes. Stanulović, V.S., Kyrmizi, I., Kruithof-de Julio, M., Hoogenkamp, M., Vermeulen, J.L., Ruijter, J.M., Talianidis, I., Hakvoort, T.B., Lamers, W.H. Hepatology (2007) [Pubmed]
  4. Mechanism of glucocorticoid induction of the rat plasminogen activator inhibitor-1 gene in HTC rat hepatoma cells: identification of cis-acting regulatory elements. Bruzdzinski, C.J., Johnson, M.R., Goble, C.A., Winograd, S.S., Gelehrter, T.D. Mol. Endocrinol. (1993) [Pubmed]
  5. Identification and characterization of the GC-rich and cyclic adenosine 3',5'-monophosphate (cAMP)-inducible promoter of the type II beta cAMP-dependent protein kinase regulatory subunit gene. Kurten, R.C., Levy, L.O., Shey, J., Durica, J.M., Richards, J.S. Mol. Endocrinol. (1992) [Pubmed]
  6. Tissue-specific differences in the binding of nuclear proteins to a CCAAT motif in the promoter of the androgen-regulated C3 gene. Zhang, Y.L., Parker, M.G., Bakker, O. Mol. Endocrinol. (1990) [Pubmed]
  7. DNase-I hypersensitivity analysis of the L-type pyruvate kinase gene in rats and transgenic mice. Boquet, D., Vaulont, S., Tremp, G., Ripoche, M.A., Daegelen, D., Jami, J., Kahn, A., Raymondjean, M. Eur. J. Biochem. (1992) [Pubmed]
  8. Comparative characterization of rat deoxyribonuclease 1 (Dnase1) and murine deoxyribonuclease 1-like 3 (Dnase1l3). Napirei, M., Wulf, S., Eulitz, D., Mannherz, H.G., Kloeckl, T. Biochem. J. (2005) [Pubmed]
  9. Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split. Ishibashi, M., Sasai, Y., Nakanishi, S., Kageyama, R. Eur. J. Biochem. (1993) [Pubmed]
  10. Lipopolysaccharide-induced fever is dissociated from apoptotic cell death in the rat brain. Mouihate, A., Pittman, Q.J. Brain Res. (1998) [Pubmed]
  11. Induction of the glucokinase gene by insulin in cultured neonatal rat hepatocytes. Relationship with DNase-I hypersensitive sites and functional analysis of a putative insulin-response element. Parsa, R., Decaux, J.F., Bossard, P., Robey, B.R., Magnuson, M.A., Granner, D.K., Girard, J. Eur. J. Biochem. (1996) [Pubmed]
  12. Purification and immunohistochemical study of actin in mitochondrial matrix. Etoh, S., Matsui, H., Tokuda, M., Itano, T., Nakamura, M., Hatase, O. Biochem. Int. (1990) [Pubmed]
  13. Characterization of deoxyribonucleic acid-protein interactions at a growth hormone-inducible nuclease hypersensitive site in the rat insulin-like growth factor-I gene. Thomas, M.J., Kikuchi, K., Bichell, D.P., Rotwein, P. Endocrinology (1995) [Pubmed]
  14. Evidence for the transcription os physiologically inactive rat-liver nucleolar chromatin by Escherichia coli RNA polymerase. Yu, F.L., Barrett, A. Biosci. Rep. (1982) [Pubmed]
 
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