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Gene Review

Testin  -  testin gene

Rattus norvegicus

Synonyms: CMB-23, Testin-2
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Disease relevance of Testin


High impact information on Testin


Biological context of Testin


Anatomical context of Testin

  • Testin I (M(r) 35,000) and testin II (M(r) 37,000) (testins) are two structurally and immunologically related testicular proteins that are actively synthesized and secreted by Sertoli cells [6].
  • Testin was localized at the base of the seminiferous epithelium at Sertoli-Sertoli junctions [3].
  • Testin was also found in the junctions between adjacent germinal epithelial cells that covered the surface of the ovary, at the lower or the lower lateral borders of each cell [4].
  • Other physiological studies have shown that the secretion of testin is tightly coupled to the presence of germ cells [7].
  • Rat testin is a newly identified component of the junctional complexes in various tissues whose mRNA is predominantly expressed in the testis and ovary [7].

Associations of Testin with chemical compounds

  • However, when the inter-testicular cell junctions were disrupted either by a drug treatment such as lonidamine in vivo or by a physical treatment in vitro such as exposing Sertoli-germ cell co-cultures where specialized junctions were formed to a hypotonic treatment, a drastic surge in the testin gene expression was noted [8].
  • CMB-22 has a pI of 5.8; CMB-23 has two distinctive isoelectric variants with pIs of 5.4 and 5.2, the latter variant was designated CMB-23 Isoform [1].
  • Basal testin expression remained stable during the 27-h incubation with actinomycin D but required de novo protein synthesis in vitro [5].
  • Sertolin and testin, the gene makers for cell-cell interactions were differentially modulated upon TCDD treatment [9].

Other interactions of Testin

  • CMB-22 and CMB-23 are highly concentrated in testes and their levels in this tissue increase with age [10].

Analytical, diagnostic and therapeutic context of Testin


  1. Identification of two testosterone-responsive testicular proteins in Sertoli cell-enriched culture medium whose secretion is suppressed by cells of the intact seminiferous tubule. Cheng, C.Y., Bardin, C.W. J. Biol. Chem. (1987) [Pubmed]
  2. Testin secreted by Sertoli cells is associated with the cell surface, and its expression correlates with the disruption of Sertoli-germ cell junctions but not the inter-Sertoli tight junction. Grima, J., Wong, C.C., Zhu, L.J., Zong, S.D., Cheng, C.Y. J. Biol. Chem. (1998) [Pubmed]
  3. Testins are localized to the junctional complexes of rat Sertoli and epididymal cells. Zong, S.D., Bardin, C.W., Phillips, D., Cheng, C.Y. Biol. Reprod. (1992) [Pubmed]
  4. Immunohistochemical localization of testin in the female reproductive system of the rat is consistent with its involvement in the turnover of specialized junctional complexes. Zhu, L., Moo-Young, A., Bardin, C.W., Cheng, C.Y. Biol. Reprod. (1997) [Pubmed]
  5. Testin induction: the role of cyclic 3',5'-adenosine monophosphate/protein kinase A signaling in the regulation of basal and lonidamine-induced testin expression by rat sertoli cells. Grima, J., Cheng, C.Y. Biol. Reprod. (2000) [Pubmed]
  6. Testins are structurally related to the mouse cysteine proteinase precursor but devoid of any protease/anti-protease activity. Cheng, C.Y., Morris, I., Bardin, C.W. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
  7. Rat testin is a newly identified component of the junctional complexes in various tissues whose mRNA is predominantly expressed in the testis and ovary. Grima, J., Zhu, L.J., Zong, S.D., Catterall, J.F., Bardin, C.W., Cheng, C.Y. Biol. Reprod. (1995) [Pubmed]
  8. Testin is tightly associated with testicular cell membrane upon its secretion by sertoli cells whose steady-state mRNA level in the testis correlates with the turnover and integrity of inter-testicular cell junctions. Grima, J., Zhu, L., Cheng, C.Y. J. Biol. Chem. (1997) [Pubmed]
  9. Effects of TCDD in modulating the expression of Sertoli cell secretory products and markers for cell-cell interaction. Lai, K.P., Wong, M.H., Wong, C.K. Toxicology (2005) [Pubmed]
  10. Testins are structurally related Sertoli cell proteins whose secretion is tightly coupled to the presence of germ cells. Cheng, C.Y., Grima, J., Stahler, M.S., Lockshin, R.A., Bardin, C.W. J. Biol. Chem. (1989) [Pubmed]
  11. Ability of trypsin in mimicking germ cell factors that affect Sertoli cell secretory function. Aravindan, G.R., Pineau, C.P., Bardin, C.W., Cheng, C.Y. J. Cell. Physiol. (1996) [Pubmed]
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