The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

aesT  -  esterase

Acinetobacter sp. ADP1

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of aesT


High impact information on aesT


Biological context of aesT

  • The original esterase-positive plasmid pRA17 carried a 2.2-kb insert from a partial MboI digest of RAG-1 DNA, which gave a single band with RAG-1 DNA following Southern hybridization [2].
  • The 27 lipase-deficient transconjugants which were isolated exhibited several different phenotypes, including gelatinase mutants, esterase mutants, and putative auxotrophs [9].
  • The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence [10].
  • These cells also produced an esterase (measured by the hydrolysis of beta-naphthyl acetate) [11].
  • The rRNA gene restriction patterns (using EcoRI and PvuII as restriction endonucleases) and the esterase electrophoretic profiles were determined on 31 strains, using as comparison strain isolates from another intensive care unit of our hospital and from two other French hospitals [4].

Anatomical context of aesT

  • The localization of hydrolytic enzymes, phosphatase, esterase, lipase and palmitoyl-CoA hydrolase was analysed in the cytosol, cytoplasmic membrane, periplasmic fraction, outer membrane and culture supernatant in dependence on the growth rate of the bacteria [12].

Associations of aesT with chemical compounds

  • Its product, SalE, was shown to have esterase activity against short-chain alkyl esters of 4-nitrophenol but was also able to hydrolyze ethyl salicylate to ethanol and salicylic acid [8].
  • Insertional inactivation of xcpR in A. calcoaceticus ADP1 by transcriptional fusion to lacZ abolishes secretion of lipase and esterase and leads to lack of growth on dodecane and slower growth on hexadecane [13].
  • Comparison with homologous proteins from both eukaryotic and prokaryotic organisms suggest that the RAG-1 esterase exhibits sequence motifs characteristic of both serine proteases and of lipases [10].
  • The role of esterase in the release of cell-bound emulsifier and the contribution of capsular polysaccharide to the emulsification activity were observed [14].
  • Each esterase was defined by its spectrum of hydrolytic activity toward five synthetic substrates and its sensitivity to di-isopropyl fluorophosphate [15].

Physical interactions of aesT

  • Deletion of estA only partially abolished cell-bound esterase activity in A. calcoaceticus, indicating that BD413 forms at least two esterases [16].

Other interactions of aesT


Analytical, diagnostic and therapeutic context of aesT


  1. An exocellular protein from the oil-degrading microbe Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan. Bach, H., Berdichevsky, Y., Gutnick, D. Appl. Environ. Microbiol. (2003) [Pubmed]
  2. Cloning and expression in Escherichia coli of an esterase-coding gene from the oil-degrading bacterium Acinetobacter calcoaceticus RAG-1. Reddy, P.G., Allon, R., Mevarech, M., Mendelovitz, S., Sato, Y., Gutnick, D.L. Gene (1989) [Pubmed]
  3. Detection of salmonellae by using Rambach agar and by a C8 esterase spot test. Freydiere, A.M., Gille, Y. J. Clin. Microbiol. (1991) [Pubmed]
  4. Biotyping, ribotyping and esterase electrophoresis as epidemiological tools for Acinetobacter baumannii. Sire, J.M., Gras-Rouzet, S., Pouëdras, P., Mesnard, R., Arvieux, C., Jenek, J., Thomas, R., Avril, J.L. Res. Microbiol. (1994) [Pubmed]
  5. Purification and characterization of benzaldehyde dehydrogenase I from Acinetobacter calcoaceticus. Chalmers, R.M., Fewson, C.A. Biochem. J. (1989) [Pubmed]
  6. Benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase II from Acinetobacter calcoaceticus. Substrate specificities and inhibition studies. MacKintosh, R.W., Fewson, C.A. Biochem. J. (1988) [Pubmed]
  7. Genes for chlorogenate and hydroxycinnamate catabolism (hca) are linked to functionally related genes in the dca-pca-qui-pob-hca chromosomal cluster of Acinetobacter sp. strain ADP1. Smith, M.A., Weaver, V.B., Young, D.M., Ornston, L.N. Appl. Environ. Microbiol. (2003) [Pubmed]
  8. sal genes determining the catabolism of salicylate esters are part of a supraoperonic cluster of catabolic genes in Acinetobacter sp. strain ADP1. Jones, R.M., Pagmantidis, V., Williams, P.A. J. Bacteriol. (2000) [Pubmed]
  9. Transposon mutagenesis in Acinetobacter calcoaceticus RAG-1. Leahy, J.G., Jones-Meehan, J.M., Pullias, E.L., Colwell, R.R. J. Bacteriol. (1993) [Pubmed]
  10. Esterase from the oil-degrading Acinetobacter lwoffii RAG-1: sequence analysis and over-expression in Escherichia coli. Alon, R.N., Gutnick, D.L. FEMS Microbiol. Lett. (1993) [Pubmed]
  11. Lipase and esterase formation by psychrophilic and mesophilic Acinetobacter species. Breuil, C., Kushner, D.J. Can. J. Microbiol. (1975) [Pubmed]
  12. Localization of hydrolytic enzymes in Acinetobacter calcoaceticus. Fischer, B.E. J. Basic Microbiol. (1986) [Pubmed]
  13. Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1. Parche, S., Geissdörfer, W., Hillen, W. J. Bacteriol. (1997) [Pubmed]
  14. Studies on bioemulsifier production by Acinetobacter strains isolated from healthy human skin. Patil, J.R., Chopade, B.A. J. Appl. Microbiol. (2001) [Pubmed]
  15. Epidemiological typing of Acinetobacter strains by esterase electrophoresis. Picard, B., Goullet, P. FEMS Microbiol. Lett. (1990) [Pubmed]
  16. Growth-phase-dependent expression of the lipolytic system of Acinetobacter calcoaceticus BD413: cloning of a gene encoding one of the esterases. Kok, R.G., Christoffels, V.M., Vosman, B., Hellingwerf, K.J. J. Gen. Microbiol. (1993) [Pubmed]
  17. A gene of Acinetobacter calcoaceticus BD413 encodes a periplasmic peptidyl-prolyl cis-trans isomerase of the cyclophilin sub-class that is not essential for growth. Kok, R.G., Christoffels, V.M., Vosman, B., Hellingwerf, K.J. Biochim. Biophys. Acta (1994) [Pubmed]
  18. Effects of low temperature, cold shock, and various carbon sources on esterase and lipase activities and exopolysaccharide production by a psychrotrophic Acinetobacter sp. Barbaro, S.E., Trevors, J.T., Inniss, W.E. Can. J. Microbiol. (2001) [Pubmed]
WikiGenes - Universities