Disease relevance of Mtap1a

• In adults, microtubule-associated protein-1a and microtubule-associated protein-1b are present in all sciatic nerve axons and in motor and dorsal root ganglion neurons [1].
• Furthermore, transection of ganglion cell axons, a procedure that causes retrograde degeneration of many of the axotomized ganglion cells, led to a decrease in the number of anti-MAP 1A immunoreactive cells in retinal wholemounts [2].
• In vivo growth suppression of rat C6 glioma transplanted in rat brain using antisense oligonucleotide for microtubule-associated protein 1A messenger ribonucleic acid [3].

High impact information on Mtap1a

• There was no particular MT which bound either MAP1A or MAP2 alone [4].
• To learn which of the MAPs were present in the microtubule bundles in the axon, sections of axons from IDPN-treated rats were examined by immunofluorescence microscopy using antibodies to MAP 1A, MAP 1B, MAP 2, and tubulin [5].
• We believe that MAP 1A and MAP 1B are major components of the microtubule-associated fibrillar matrix in the axon [5].
• To determine the distribution of MAP 1A in nervous system tissues and cells, we examined tissue sections from rat brain and spinal cord, as well as primary cultures of newborn rat brain by immunofluorescence microscopy [6].
• These results indicate that in contrast to MAP 2, which is specifically enriched in dendrites and perikarya of neurons, MAP 1A is widely distributed in the nervous system [6].

Biological context of Mtap1a

• During embryogenesis microtubule-associated protein-1b and tau are present in all neurons and axons, microtubule-associated protein-2 is present in neurons but not in axons, and there is no microtubule-associated protein-1a [1].
• The up-regulation of microtubule associated protein 1A (MAP1A) in one of these was confirmed by real-time polymerase chain reaction [7].
• Whereas MAP-1B, MAP-2, and beta-tubulin are phosphorylated in the brain of 5-day-old rat pups, when most neurons of the CNS are extending processes, MAP-1A phosphorylation is observed only after neuronal maturation takes place [8].
• The molecular structure of MAP1A was further investigated by the rotary shadowing technique [9].
• This study strongly suggests the important role of MAP 1A in cell proliferation and its suppressionmay serve as a novel antitumour therapy for gliomas [3].

Anatomical context of Mtap1a

• Colocalization of microtubule-associated protein 1A and microtubule-associated protein 2 on neuronal microtubules in situ revealed with double-label immunoelectron microscopy [4].
• These studies identify a novel interaction between the C-terminal tail of the BKCa channel and the light chain of MAP1A, which enables channel association with and modulation by the cytoskeleton [10].
• Microtubule associated protein (MAP1A) mRNA was up-regulated by hypergravity in the rat inner ear [7].
• We have searched for a microtubule-binding domain by expressing different protein regions of MAP1A in cultured cell lines using cDNA constructs [11].
• During further development, the intensity of the MAP1a staining in the spinal columns gradually decreased [12].

Associations of Mtap1a with chemical compounds

• We show here that one effect of estrogen is to promote an increase in tau, but not in tubulin, microtubule-associated protein 1a (MAP-1a), or MAP-2 protein levels [13].
• The phosphorylation of MAP-1A, MAP-1B, and beta-tubulin may be due mainly to casein kinase II or a related enzyme, whereas MAP-2 appears to be modified by other enzymes such as the cyclic AMP-dependent protein kinase (protein kinase A) and the calcium/phospholipid-dependent protein kinase (protein kinase C) [8].
• Microtubule-associated protein (MAP) 1 consisting of MAP 1A and 1B was purified from rat brain by the poly-L-aspartic acid (PLAA) method [14].
• The mean diameters of the tumours from rats treated with antisense, sense and scramble phosphorothioate oligodeoxynucleotide for MAP 1A mRNA were 2.33, 4.625 and 5.25 mm [3].

Other interactions of Mtap1a

• We found that MAP 1 can be resolved by SDS PAGE into three electrophoretic bands, which we have designated MAP 1A, MAP 1B, and MAP 1C in order of increasing electrophoretic mobility [6].
• The identified cluster includes neuritin, attractin, microtubule-associated protein 1a, and myelin oligodendrocyte protein genes [15].

Analytical, diagnostic and therapeutic context of Mtap1a

• Following 6 h and 3, 7 and 14 days post-implantation survival, the brains were removed and paraffin sections were processed for hematoxylin-eosin staining, as well as immunohistochemistry for microtubule-associated protein (MAP-1A) and glial fibrillary acidic protein [16].
• After 7 days from transplantation, antisense, sense or scramble phosphorothioate oligodeoxynucleotide for MAP 1A mRNA was gradually delivered into the established tumours through amini-osmotic pump [3].

References