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Gene Review

Rnaseh1  -  ribonuclease H1

Rattus norvegicus

Synonyms: RNase H1, Ribonuclease H1
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Disease relevance of Rnaseh1


High impact information on Rnaseh1

  • Ribonucleotide-rich regions can be degraded to generate partially single-stranded molecules by RNase H treatment in vitro or during DNA extraction from crude mitochondria [3].
  • Poly(A)- RNA and poly(A)+ RNA from which tails were removed by RNase H digestion were much more effective than poly(A)+ RNA in expressing AVP in the magnocellular hypothalamic neurons and in raising urine osmolarity [4].
  • The present study investigates the protein binding site within the 27-base HIPBS, first by using specific cleavages of HIPBS and its flanking sequences with antisense oligodeoxynucleotides and RNase H and then by using mutational analysis of the binding properties [5].
  • In addition to the previously identified DNA polymerase alpha, DNA primase, 3'-5' exonuclease, RNase H, and DNA methylase were all recovered at significant levels (20-30% of total nuclear activity) in nuclear matrix isolated from regenerating rat liver during maximal in vivo replication (22 h post-hepatectomy) [6].
  • Mild sonication resulted in a significant release of all of these activities except RNase H. A major portion of the matrix-solubilized DNA polymerase alpha, DNA primase, 3'-5' exonuclease, and DNA methylase activities cosedimented on sucrose gradients between approximately 8-12 S [6].

Biological context of Rnaseh1

  • Moreover, protein binding to this fragment was prevented with an antisense oligonucleotide directed against bases 1551-1579 and subsequent RNase H digestion [7].
  • In vivo transfection of antisense ACE ODNs into intact uninjured rat carotid artery resulted in a significant reduction of vascular ACE activity, and cotransfection of HMG-1 and RNase H showed further reduction [8].
  • A series of oligonucleotide-directed ribonuclease H mapping experiments determined that the two mRNA species differ at their 3' ends in the extent of polyadenylation [9].
  • Inhibition of c-myc was specifically achieved by using phosphorodiamidate Morpholino oligomer (PMOs), a novel, non-toxic antisense DNA chemistry for inhibition of gene expression by an RNase H-independent mechanism [10].
  • Initial experiments using oligo (dT)-directed RNase H hydrolysis indicated that the two mRNAs have poly [A+] tails of identical length [11].

Anatomical context of Rnaseh1

  • Removal of the ribonucleotides with calf thymus ribonuclease H, followed by denaturation of the mitochondrial DNA and analysis of the single-strand fragment lengths in the electron microscope, showed that the ribonucleotides were randomly located on both strands of the DNA [12].
  • Multiple forms of ribonuclease H from rat liver cytosol [13].
  • These results indicate that multiple forms of ribonuclease H exist in different regions of rat liver cells [14].

Associations of Rnaseh1 with chemical compounds


Enzymatic interactions of Rnaseh1

  • RNase H was used to hydrolyze IGF-I mRNAs at the point of annealment with the oligomers [18].

Other interactions of Rnaseh1

  • RNase H cleavage and Northern blot analysis of the 3'-ends established that rapid deadenylation occurred concomitantly with the rapid decay of the betaG-GA mRNA in cells grown in normal medium [19].
  • By contrast, RNase H analysis revealed a transient, T3-induced increase in P450R mRNA poly(A) tail, from approximately 100 to approximately 205 A [20].
  • RNase protection, primer extension, RACE PCR and ribonuclease H mapping established the complete structure of the 5' end of class 1 and class 2 IGF-I mRNAs [21].
  • Both alpha 1b-adrenergic receptor mRNAs appear to share extensive regions of homology, therefore, we used oligonucleotide-directed ribonuclease H mapping to detect sequence differences between the two transcripts [11].

Analytical, diagnostic and therapeutic context of Rnaseh1

  • We compared, in this study, the structure of mRNAs encoding SPTm and SPTp by comparison of the sizes after removal of poly(A) tail by ribonuclease H and by means of RNA blot analysis and S1 nuclease protection assay [22].
  • To determine whether these changes occurred in the 5' (alternate transcriptional start site or splicing)- or 3' (altered polyadenylation)-end of the molecules, mRNAs were cleaved using oligonucleotide-directed RNase-H digestion, and the fragments were analyzed by Northern blot, using probes specific to the 5'- and 3'-segments of each transcript [23].
  • We have used primer extension analysis, reverse transcriptase associated with polymerase chain reaction, and RNase H digestion to show that these two mRNA species have the same structural features, including 5'- and 3'-ends, and poly(A) tail length [24].
  • Two forms of RNase H were separated from rat liver nuclei by affinity chromatography using a DNA-cellulose column [17].


  1. Use of thermostable and Escherichia coli RNase H in RNA mapping studies. Porter, D., Curthoys, N.P. Anal. Biochem. (1997) [Pubmed]
  2. Ribonuclease H activity in rat mammary gland during the lactation cycle and in R3230AC mammary adenocarcinoma. Orloski, J.M., Fritz, P.J., Liu, D.K. Biochim. Biophys. Acta (1980) [Pubmed]
  3. Biased incorporation of ribonucleotides on the mitochondrial L-strand accounts for apparent strand-asymmetric DNA replication. Yang, M.Y., Bowmaker, M., Reyes, A., Vergani, L., Angeli, P., Gringeri, E., Jacobs, H.T., Holt, I.J. Cell (2002) [Pubmed]
  4. Reduction of exogenous vasopressin RNA poly(A) tail length increases its effectiveness in transiently correcting diabetes insipidus in the Brattleboro rat. Maciejewski-Lenoir, D., Jirikowski, G.F., Sanna, P.P., Bloom, F.E. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  5. Characterization of the hypoxia-inducible protein binding site within the pyrimidine-rich tract in the 3'-untranslated region of the tyrosine hydroxylase mRNA. Czyzyk-Krzeska, M.F., Beresh, J.E. J. Biol. Chem. (1996) [Pubmed]
  6. Pre-replicative association of multiple replicative enzyme activities with the nuclear matrix during rat liver regeneration. Tubo, R.A., Berezney, R. J. Biol. Chem. (1987) [Pubmed]
  7. Hypoxia stimulates binding of a cytoplasmic protein to a pyrimidine-rich sequence in the 3'-untranslated region of rat tyrosine hydroxylase mRNA. Czyzyk-Krzeska, M.F., Dominski, Z., Kole, R., Millhorn, D.E. J. Biol. Chem. (1994) [Pubmed]
  8. Antisense oligodeoxynucleotide inhibition of vascular angiotensin-converting enzyme expression attenuates neointimal formation: evidence for tissue angiotensin-converting enzyme function. Morishita, R., Gibbons, G.H., Tomita, N., Zhang, L., Kaneda, Y., Ogihara, T., Dzau, V.J. Arterioscler. Thromb. Vasc. Biol. (2000) [Pubmed]
  9. Developmentally regulated polyadenylation of two discrete messenger ribonucleic acids for müllerian inhibiting substance. Lee, M.M., Cate, R.L., Donahoe, P.K., Waneck, G.L. Endocrinology (1992) [Pubmed]
  10. Resistance to chemotherapeutic drugs overcome by c-Myc inhibition in a Lewis lung carcinoma murine model. Knapp, D.C., Mata, J.E., Reddy, M.T., Devi, G.R., Iversen, P.L. Anticancer Drugs (2003) [Pubmed]
  11. Two alpha 1b-adrenergic receptor mRNAS expressed in Sprague-Dawley rat liver have distinct 5'-regions. Deng, C.L., Cornett, L.E. J. Recept. Res. (1994) [Pubmed]
  12. Localization of the ribonucleotide sites in rat liver mitochondrial deoxyribonucleic acid. Lonsdale, D.M., Jones, I.G. Biochem. J. (1978) [Pubmed]
  13. Multiple forms of ribonuclease H from rat liver cytosol. Sawai, Y., Yanokura, M., Tsukada, K. J. Biochem. (1979) [Pubmed]
  14. Ribonuclease H from rat liver. I. Partial purification and characterization of nuclear ribonuclease H1. Tashiro, F., Ueno, Y. J. Biochem. (1978) [Pubmed]
  15. Interaction of mammalian deoxyribonuclease V, a double strand 3' to 5' and 5' to 3' exonuclease, with deoxyribonucleic acid polymerase-beta from the Novikoff hepatoma. Mosbaugh, D.W., Meyer, R.R. J. Biol. Chem. (1980) [Pubmed]
  16. Change of ribonuclease H activity from rat liver nuclei by thioacetamide: preferential increase in the activity of magnesium-dependent ribonuclease H. Tsukada, K., Sawai, Y., Saito, J., Sato, F. Biochem. Biophys. Res. Commun. (1978) [Pubmed]
  17. Nuclear location of ribonuclease H and increased level of magnesium-dependent ribonuclease H in rat liver on thioacetamide treatment. Sawai, Y., Kitahara, N., Thung, W.L., Yanokura, M., Tsukada, K. J. Biochem. (1981) [Pubmed]
  18. The size heterogeneity of rat insulin-like growth factor-I mRNAs is due primarily to differences in the length of 3'-untranslated sequence. Lund, P.K., Hoyt, E.C., Van Wyk, J.J. Mol. Endocrinol. (1989) [Pubmed]
  19. Role of deadenylation and AUF1 binding in the pH-responsive stabilization of glutaminase mRNA. Schroeder, J.M., Ibrahim, H., Taylor, L., Curthoys, N.P. Am. J. Physiol. Renal Physiol. (2006) [Pubmed]
  20. Post-transcriptional regulation of hepatic NADPH-cytochrome P450 reductase by thyroid hormone: independent effects on poly(A) tail length and mRNA stability. Liu, D., Waxman, D.J. Mol. Pharmacol. (2002) [Pubmed]
  21. Multiple transcription start sites in the rat insulin-like growth factor-I gene give rise to IGF-I mRNAs that encode different IGF-I precursors and are processed differently in vitro. Simmons, J.G., Van Wyk, J.J., Hoyt, E.C., Lund, P.K. Growth Factors (1993) [Pubmed]
  22. Generation from a single gene of two mRNAs that encode the mitochondrial and peroxisomal serine:pyruvate aminotransferase of rat liver. Oda, T., Funai, T., Ichiyama, A. J. Biol. Chem. (1990) [Pubmed]
  23. Pulsatile gonadotropin-releasing hormone modifies polyadenylation of gonadotropin subunit messenger ribonucleic acids. Weiss, J., Crowley, W.F., Jameson, J.L. Endocrinology (1992) [Pubmed]
  24. Calbindin-D9k gene expression in the uterus: study of the two messenger ribonucleic acid species and analysis of an imperfect estrogen-responsive element. L'Horset, F., Blin, C., Colnot, S., Lambert, M., Thomasset, M., Perret, C. Endocrinology (1994) [Pubmed]
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