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Gene Review

pilT  -  transglycosylase

Escherichia coli

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Disease relevance of pilT


High impact information on pilT


Chemical compound and disease context of pilT


Biological context of pilT

  • Restoration of the normal phenotypes is also possible by introduction of the pilT gene encoded by the unrelated IncI plasmid R64 [13].
  • Penicillin binding protein (PBP) 1b of Escherichia coli has both transglycosylase and transpeptidase activities, which are attractive targets for the discovery of new antibacterial agents [14].
  • A simple assay for detection of compounds that bind to the active site in the transglycosylation domain of the essential bifunctional transglycosylase and transpeptidase penicillin-binding proteins (PBPs) is reported [15].
  • With similar amino acid sequences and modular designs, M. leprae PBP1 is related to Escherichia coli PBP1a and PBP1b, bienzymatic proteins with transglycosylase and transpeptidase activities [16].
  • (X = N-acetylglucosaminyl-1,6-anhydro-N-acetylmuramyl-L-alanyl-D-gamma-glutamyl-meso-diaminopimelic acid; X' = X-D-alanine.) The kinetics of the activity of the membrane-bound enzyme were found to be identical to that of the soluble enzyme, indicating that both molecular species of transglycosylase function as exoenzymes in vitro [17].

Associations of pilT with chemical compounds


Other interactions of pilT

  • The pilK, pilR, and pilT products were produced with a C-terminal His tag and then detected by anti-His tag antibody [21].

Analytical, diagnostic and therapeutic context of pilT


  1. Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women. Baranowski, W., Dirheimer, G., Jakowicki, J.A., Keith, G. Cancer Res. (1994) [Pubmed]
  2. Role of the Gp16 lytic transglycosylase motif in bacteriophage T7 virions at the initiation of infection. Moak, M., Molineux, I.J. Mol. Microbiol. (2000) [Pubmed]
  3. Specificity of induction of glycopeptide resistance genes in Enterococcus faecalis. Baptista, M., Depardieu, F., Courvalin, P., Arthur, M. Antimicrob. Agents Chemother. (1996) [Pubmed]
  4. Inhibition of membrane-bound lytic transglycosylase B by NAG-thiazoline. Reid, C.W., Blackburn, N.T., Legaree, B.A., Auzanneau, F.I., Clarke, A.J. FEBS Lett. (2004) [Pubmed]
  5. Staphylococcus aureus and Micrococcus luteus peptidoglycan transglycosylases that are not penicillin-binding proteins. Park, W., Matsuhashi, M. J. Bacteriol. (1984) [Pubmed]
  6. The lethal lambda S gene encodes its own inhibitor. Bläsi, U., Chang, C.Y., Zagotta, M.T., Nam, K.B., Young, R. EMBO J. (1990) [Pubmed]
  7. Cloning and characterization of PBP 1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli. Schiffer, G., Höltje, J.V. J. Biol. Chem. (1999) [Pubmed]
  8. Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein. Ishino, F., Park, W., Tomioka, S., Tamaki, S., Takase, I., Kunugita, K., Matsuzawa, H., Asoh, S., Ohta, T., Spratt, B.G. J. Biol. Chem. (1986) [Pubmed]
  9. Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase. Nakagawa, J., Tamaki, S., Tomioka, S., Matsuhashi, M. J. Biol. Chem. (1984) [Pubmed]
  10. Screen for Inhibitors of the Coupled Transglycosylase-Transpeptidase of Peptidoglycan Biosynthesis in Escherichia coli. Ramachandran, V., Chandrakala, B., Kumar, V.P., Usha, V., Solapure, S.M., de Sousa, S.M. Antimicrob. Agents Chemother. (2006) [Pubmed]
  11. Changes in amount of hypo-modified tRNA having guanine in place of queuine during erythroid differentiation of murine erythroleukemia cells. Shindo-Okada, N., Terada, M., Nishimura, S. Eur. J. Biochem. (1981) [Pubmed]
  12. Analysis of the different molecular forms of penicillin-binding protein 1B in Escherichia coli ponB mutants lysogenized with specialized transducing lambda (ponB+) bacteriophages. Rojo, F., Ayala, J.A., De Pedro, M.A., Vázquez, D. Eur. J. Biochem. (1984) [Pubmed]
  13. Gene 19 of plasmid R1 is required for both efficient conjugative DNA transfer and bacteriophage R17 infection. Bayer, M., Eferl, R., Zellnig, G., Teferle, K., Dijkstra, A., Koraimann, G., Högenauer, G. J. Bacteriol. (1995) [Pubmed]
  14. High-throughput screen for inhibitors of transglycosylase and/or transpeptidase activities of Escherichia coli penicillin binding protein 1b. Chandrakala, B., Shandil, R.K., Mehra, U., Ravishankar, S., Kaur, P., Usha, V., Joe, B., deSousa, S.M. Antimicrob. Agents Chemother. (2004) [Pubmed]
  15. A simple screen for murein transglycosylase inhibitors. Vollmer, W., Höltje, J.V. Antimicrob. Agents Chemother. (2000) [Pubmed]
  16. Identification and overexpression in Escherichia coli of a Mycobacterium leprae gene, pon1, encoding a high-molecular-mass class A penicillin-binding protein, PBP1. Basu, J., Mahapatra, S., Kundu, M., Mukhopadhyay, S., Nguyen-Distèche, M., Dubois, P., Joris, B., Van Beeumen, J., Cole, S.T., Chakrabarti, P., Ghuysen, J.M. J. Bacteriol. (1996) [Pubmed]
  17. Exoenzymatic activity of transglycosylase isolated from Escherichia coli. Beachey, E.H., Keck, W., de Pedro, M.A., Schwarz, U. Eur. J. Biochem. (1981) [Pubmed]
  18. The structure and function of Escherichia coli penicillin-binding protein 3. Nguyen-Distèche, M., Fraipont, C., Buddelmeijer, N., Nanninga, N. Cell. Mol. Life Sci. (1998) [Pubmed]
  19. Mutations in the Escherichia coli fnr and tgt genes: control of molybdate reductase activity and the cytochrome d complex by fnr. Frey, B., Jänel, G., Michelsen, U., Kersten, H. J. Bacteriol. (1989) [Pubmed]
  20. Microplate Assay for Inhibitors of the Transpeptidase Activity of PBP1b of Escherichia coli. Jha, R.K., de Sousa, S.M. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening (2006) [Pubmed]
  21. Genes required for plasmid R64 thin-pilus biogenesis: identification and localization of products of the pilK, pilM, pilO, pilP, pilR, and pilT genes. Sakai, D., Komano, T. J. Bacteriol. (2002) [Pubmed]
  22. Escherichia coli murein transglycosylase. Purification by affinity chromatography and interaction with polynucleotides. Kusser, W., Schwarz, U. Eur. J. Biochem. (1980) [Pubmed]
  23. Comparison of two hydrolytic murein transglycosylases of Escherichia coli. Keck, W., Wientjes, F.B., Schwarz, U. Eur. J. Biochem. (1985) [Pubmed]
  24. A surface plasmon resonance analysis of the interaction between the antibiotic moenomycin A and penicillin-binding protein 1b. Stembera, K., Vogel, S., Buchynskyy, A., Ayala, J.A., Welzel, P. Chembiochem (2002) [Pubmed]
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