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Gene Review

ccnb1  -  cyclin B1

Xenopus laevis

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High impact information on ccnb1


Biological context of ccnb1


Anatomical context of ccnb1

  • Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation [5].
  • This kinase activity rises more smoothly than that of the cyclin B-cdc2 complexes and reaches a peak earlier in the cell cycle; indeed, cyclin A is destroyed before nuclear envelope breakdown [6].
  • In spite of similar enzymatic properties of cdk5/p26 and p34cdc2/cyclin B kinase, cdk5/p26 did not display M-phase promoting activity when assayed with a cell-free system of Xenopus egg extract [7].
  • However, the cyclin A-dependent kinase does not induce a dramatic shortening of centrosome-nucleated microtubules whereas the cyclin B-dependent kinase does, as previously reported [8].
  • We have partially characterized this inhibitory pathway as one involving a reversible binding inhibitor of p34cdc2/cyclin B that is tightly associated with cell membranes [9].

Associations of ccnb1 with chemical compounds

  • When the blastomeres were treated with nocodazole or latrunculin A at the 8th cell cycle, the amount of cortical cdc2 decreased, but that of cyclin B did not change [3].
  • The 26S, but not the 20S proteasome, digested recombinant 49-kD cyclin B at lysine 57 (K57), producing a 42-kD truncated form [10].
  • The results of this study indicate that the destruction of cyclin B is initiated by the ATP-dependent and ubiquitin-independent proteolytic activity of 26S proteasome through the first cutting in the NH2 terminus of cyclin (at K57 in the case of goldfish cyclin B) [10].
  • However, when all of the Ser phosphorylation sites in the CRS are mutated to Ala to abolish phosphorylation, the mutant cyclin B1Ala is inactivated; activity can be enhanced by mutation of these residues to Glu to mimic phosphoserine, suggesting that phosphorylation of cyclin B1 is required for its biological activity [11].
  • Several of the cdc2 mutants were found to affect the kinetics of cyclin B1 and/or mos-induced GVBD upon coinjection, although none affected the rate of progesterone-induced maturation [12].

Physical interactions of ccnb1

  • The 42-kD cyclin was also produced by the digestion of native cyclin B forming a complex with cdc2, a catalytic subunit of MPF, and a fragment transiently appeared during cyclin degradation when eggs were released from metaphase II arrest by egg activation [10].

Other interactions of ccnb1

  • Prior ablation of Cdc20, addition of methyl-ubiquitin, or addition of nondestructible Delta90 cyclin B rescues the MI-MII transition in Emi1-inhibited oocytes [13].
  • However, p34cdc2/cyclin B kinase was distinguished from cdk5/p26 by its binding to p13suc1 protein and by its reactivity to anti-p34cdc2 antibodies [7].
  • However, polyadenylation of cyclin B1 and histone B4 mRNA was still observed [14].

Analytical, diagnostic and therapeutic context of ccnb1


  1. Translational control of the embryonic cell cycle. Groisman, I., Jung, M.Y., Sarkissian, M., Cao, Q., Richter, J.D. Cell (2002) [Pubmed]
  2. CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division. Groisman, I., Huang, Y.S., Mendez, R., Cao, Q., Theurkauf, W., Richter, J.D. Cell (2000) [Pubmed]
  3. The cytoskeleton-dependent localization of cdc2/cyclin B in blastomere cortex during Xenopus embryonic cell cycle. Nakamura, N., Tokumoto, T., Ueno, S., Iwao, Y. Mol. Reprod. Dev. (2005) [Pubmed]
  4. Phosphorylation of the cyclin b1 cytoplasmic retention sequence by mitogen-activated protein kinase and Plx. Walsh, S., Margolis, S.S., Kornbluth, S. Mol. Cancer Res. (2003) [Pubmed]
  5. Cyclin B in Xenopus oocytes: implications for the mechanism of pre-MPF activation. Gautier, J., Maller, J.L. EMBO J. (1991) [Pubmed]
  6. The A- and B-type cyclin associated cdc2 kinases in Xenopus turn on and off at different times in the cell cycle. Minshull, J., Golsteyn, R., Hill, C.S., Hunt, T. EMBO J. (1990) [Pubmed]
  7. Porcine brain neurofilament-H tail domain kinase: its identification as cdk5/p26 complex and comparison with cdc2/cyclin B kinase. Hisanaga, S., Uchiyama, M., Hosoi, T., Yamada, K., Honma, N., Ishiguro, K., Uchida, T., Dahl, D., Ohsumi, K., Kishimoto, T. Cell Motil. Cytoskeleton (1995) [Pubmed]
  8. Control of microtubule dynamics and length by cyclin A- and cyclin B-dependent kinases in Xenopus egg extracts. Verde, F., Dogterom, M., Stelzer, E., Karsenti, E., Leibler, S. J. Cell Biol. (1992) [Pubmed]
  9. An inhibitor of p34cdc2/cyclin B that regulates the G2/M transition in Xenopus extracts. Lee, T.H., Kirschner, M.W. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  10. Initiation of cyclin B degradation by the 26S proteasome upon egg activation. Tokumoto, T., Yamashita, M., Tokumoto, M., Katsu, Y., Horiguchi, R., Kajiura, H., Nagahama, Y. J. Cell Biol. (1997) [Pubmed]
  11. Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation. Li, J., Meyer, A.N., Donoghue, D.J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  12. Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites. Pickham, K.M., Meyer, A.N., Li, J., Donoghue, D.J. Mol. Cell. Biol. (1992) [Pubmed]
  13. Emi1 class of proteins regulate entry into meiosis and the meiosis I to meiosis II transition in Xenopus oocytes. Tung, J.J., Jackson, P.K. Cell Cycle (2005) [Pubmed]
  14. The Mos pathway regulates cytoplasmic polyadenylation in Xenopus oocytes. de Moor, C.H., Richter, J.D. Mol. Cell. Biol. (1997) [Pubmed]
  15. Okadaic acid mimics a nuclear component required for cyclin B-cdc2 kinase microinjection to drive starfish oocytes into M phase. Picard, A., Labbé, J.C., Barakat, H., Cavadore, J.C., Dorée, M. J. Cell Biol. (1991) [Pubmed]
  16. Phosphorylation of Xenopus cyclins B1 and B2 is not required for cell cycle transitions. Izumi, T., Maller, J.L. Mol. Cell. Biol. (1991) [Pubmed]
  17. In vivo regulation of cytostatic activity in Xenopus metaphase II-arrested oocytes. Thibier, C., De Smedt, V., Poulhe, R., Huchon, D., Jessus, C., Ozon, R. Dev. Biol. (1997) [Pubmed]
  18. A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus. Pérez-Mongiovi, D., Chang, P., Houliston, E. J. Cell. Sci. (1998) [Pubmed]
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