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Gene Review

RPS14  -  ribosomal protein S14

Homo sapiens

Synonyms: 40S ribosomal protein S14, EMTB, PRO2640, S14
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Disease relevance of RPS14


High impact information on RPS14

  • In light of these data, we propose a model for fine regulation of human RPS14 transcription that involves RPS14 intron 1 antisense RNAs as positive effectors and S14 protein as a negative effector [3].
  • As judged by its resistance to high concentrations of alpha-amanitin, cell-free transcription of alpha-250 and alpha-280 appears to involve RNA polymerase I. Tissue culture transfection and cell-free transcription experiments demonstrate that alpha-250 and alpha-280 stimulate S14 mRNA transcription, whereas free ribosomal protein S14 inhibits it [3].
  • Surprisingly, the RPS14-coding region is located between the two exons [4].
  • A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: common use of the same mitochondrial targeting signal for different proteins [4].
  • Considering that the rice mitochondrial genome lacks the sdhB gene but contains the rps14-related sequence, transfer of the sdhB gene seems to have occurred before the transfer of the rps14 gene [4].

Biological context of RPS14


Anatomical context of RPS14

  • Apparently HeLa cells either initiate transcription at multiple sites within RPS14 exon 1, or capped 5' oligonucleotides are removed from most S14 mRNAs posttranscription [5].
  • Data obtained distinguish null alleles of RPS14 which encode proteins that are not incorporated into pre-ribosomal subunit particles from null alleles whose products are compatible with normal ribosome assembly processes but result in functionally inactive cytoplasmic 40 S ribosomal subunits [7].
  • Stably transformed clones were selected from cultures of emetine-resistant CHO cells (Emr-2) after transfection with pSV2Neo-human RPS14 constructs [8].
  • Transfer of RPS14 and RPL5 from the mitochondrion to the nucleus in grasses [9].

Associations of RPS14 with chemical compounds


Other interactions of RPS14

  • Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr [14].
  • To better understand the strategies developed by higher plants to transfer organellar genes during evolution, we investigated the fate of the mitochondrial RPL5-RPS14 locus in grasses [9].
  • Phylogenetic analysis of the deduced amino acid sequences demonstrated that each of the T. ruralis proteins is most similar to ribosomal proteins from higher plants even though RPS14 and RPL23 show high divergence from their other plant counterparts [15].
  • In maize, the functional gene encoding mitochondrial ribosomal protein S14 (rps14) has been translocated to the nucleus where it became integrated between both exons of a gene encoding the iron-sulfur subunit of succinate dehydrogenase (sdh2) [16].
  • These findings clearly demonstrated that Rps14, Rps16 and Rpl23 transcripts are retained within the polysomal fractions of desiccated gametophytes [15].

Analytical, diagnostic and therapeutic context of RPS14

  • Nonetheless, individual intron splice donor, splice acceptor, and upstream flanking motifs have been conserved within mammalian S14 homologues as well as within RPS14 gene fragments PCR amplified from other vertebrate genera (birds and bony fish) [17].
  • Sequence analysis of independently derived cDNA clones covering the entire rps14 coding region shows two nucleotides edited from the genomic DNA to the mRNA derived sequences by C to U modifications [18].


  1. Interactions of yeast ribosomal protein rpS14 with RNA. Antúnez de Mayolo, P., Woolford, J.L. J. Mol. Biol. (2003) [Pubmed]
  2. Possible new genetic marker for human lymphoid leukemias as detected by ribosomal protein S14 cDNA. Tasheva, E.S., Ariyasu, T., Matsuo, Y., Drexler, H.G., Minowada, J. Leukemia (1993) [Pubmed]
  3. Regulation of human RPS14 transcription by intronic antisense RNAs and ribosomal protein S14. Tasheva, E.S., Roufa, D.J. Genes Dev. (1995) [Pubmed]
  4. A single nuclear transcript encoding mitochondrial RPS14 and SDHB of rice is processed by alternative splicing: common use of the same mitochondrial targeting signal for different proteins. Kubo, N., Harada, K., Hirai, A., Kadowaki, K. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  5. Primary structure of human ribosomal protein S14 and the gene that encodes it. Rhoads, D.D., Dixit, A., Roufa, D.J. Mol. Cell. Biol. (1986) [Pubmed]
  6. Fine-structure map of the human ribosomal protein gene RPS14. Diaz, J.J., Roufa, D.J. Mol. Cell. Biol. (1992) [Pubmed]
  7. Functional analysis of human RPS14 null alleles. Martin-Nieto, J., Roufa, D.J. J. Cell. Sci. (1997) [Pubmed]
  8. A cloned human ribosomal protein gene functions in rodent cells. Rhoads, D.D., Roufa, D.J. Mol. Cell. Biol. (1987) [Pubmed]
  9. Transfer of RPS14 and RPL5 from the mitochondrion to the nucleus in grasses. Sandoval, P., León, G., Gómez, I., Carmona, R., Figueroa, P., Holuigue, L., Araya, A., Jordana, X. Gene (2004) [Pubmed]
  10. Active mammalian replication origins are associated with a high-density cluster of mCpG dinucleotides. Rein, T., Zorbas, H., DePamphilis, M.L. Mol. Cell. Biol. (1997) [Pubmed]
  11. Deoxycytidine methylation and the origin of spontaneous transition mutations in mammalian cells. Tasheva, E.S., Roufa, D.J. Somat. Cell Mol. Genet. (1993) [Pubmed]
  12. Densely methylated DNA islands in mammalian chromosomal replication origins. Tasheva, E.S., Roufa, D.J. Mol. Cell. Biol. (1994) [Pubmed]
  13. Transfer of rps14 from the mitochondrion to the nucleus in maize implied integration within a gene encoding the iron-sulphur subunit of succinate dehydrogenase and expression by alternative splicing. Figueroa, P., Gómez, I., Holuigue, L., Araya, A., Jordana, X. Plant J. (1999) [Pubmed]
  14. Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5. Dana, S., Wasmuth, J.J. Mol. Cell. Biol. (1982) [Pubmed]
  15. The translational apparatus of Tortula ruralis: polysomal retention of transcripts encoding the ribosomal proteins RPS14, RPS16 and RPL23 in desiccated and rehydrated gametophytes. Wood, A.J., Joel Duff, R., Oliver, M.J. J. Exp. Bot. (2000) [Pubmed]
  16. The nuclear-encoded SDH2-RPS14 precursor is proteolytically processed between SDH2 and RPS14 to generate maize mitochondrial RPS14. Figueroa, P., Holuigue, L., Araya, A., Jordana, X. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  17. Molecular evolution of the mammalian ribosomal protein gene, RPS14. Rhoads, D.D., Roufa, D.J. Mol. Biol. Evol. (1991) [Pubmed]
  18. Ribosomal protein S14 transcripts are edited in Oenothera mitochondria. Schuster, W., Unseld, M., Wissinger, B., Brennicke, A. Nucleic Acids Res. (1990) [Pubmed]
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