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Gene Review

UBE2C  -  ubiquitin-conjugating enzyme E2C

Homo sapiens

Synonyms: UBCH10, UbcH10, Ubiquitin carrier protein C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, ...
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Disease relevance of UBE2C


High impact information on UBE2C

  • HOXC10 appears to be a new prometaphase target of the anaphase-promoting complex (APC), since its degradation coincides with cyclin A destruction and is suppressed by expression of a dominant-negative form of UbcH10, an APC-associated ubiquitin-conjugating enzyme [4].
  • Thus, E2-C/UbcH10-mediated ubiquitination is involved in both cdc2 inactivation and sister chromatid separation, processes that are normally coordinated during exit from mitosis [5].
  • Dominant-negative clam E2-C and human UbcH10 proteins, created by altering the catalytic cysteine to serine, inhibit the in vitro ubiquitination and destruction of cyclin B in clam oocyte extracts [5].
  • When transfected into mammalian cells, mutant UbcH10 inhibits the destruction of both cyclin A and B, arrests cells in M phase, and inhibits the onset of anaphase, presumably by blocking the ubiquitin-dependent proteolysis of proteins responsible for sister chromatid separation [5].
  • To further investigate a possible contribution of UbcH10 to malignant transformation and tumor cell proliferation, NIH3T3 cells were transfected with the expression plasmid encoding UbcH10, and stable transfectants were subsequently established [6].

Biological context of UBE2C

  • Transfection of UBE2C small interfering RNA also caused inhibiton of cell proliferation and distortion of the cell cycle, with maximal increase of G(2) cells (155% of mock cells) at 72 hours and of S-phase cells (308% of mock cells) at 24 hours [2].
  • Transfection of dominant-negative UBE2C into Seg-1 cells decreased proliferation (P = .04) and increased mitotic arrest compared to vector controls (63.5% vs 6.8%; P < .001) [2].
  • The structure provides additional insight into UbcH10 function including possible sites of interaction with the anaphase promoting complex/cyclosome and the disposition of a putative destruction box motif in the structure [7].
  • We report here the 1.95-A crystal structure of a mutant UbcH10, in which the active site cysteine has been replaced with a serine [7].
  • Diminution of UbcH10 expression significantly inhibited both tumor and normal cell proliferation without inducing cell death [3].

Anatomical context of UBE2C

  • We examined the expression of ubiquitin-conjugating enzyme E2C (UBE2C) during progression from Barrett's metaplasia to esophageal adenocarcinoma (EA) and the effects of targeting this enzyme on EA-derived cell lines [2].
  • Intriguingly, UbcH10 was expressed at high levels in primary tumors derived from the lung, stomach, uterus, and bladder as compared with their corresponding normal tissues, suggesting that UbcH10 is involved in tumorigenesis or progression of the tumor [6].
  • However, when combined with agonists of the DR5/TRAIL receptor, siRNAs directed against the UbcH10 transcript dramatically enhanced killing of cancer cells, but not of proliferating primary human epithelial cells or fibroblasts [3].
  • UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas [8].

Associations of UBE2C with chemical compounds

  • UbcH10-overexpressing cells exhibited an increased incorporation of bromodeoxyuridine, an enhanced growth rate, an increase in saturation density, and a promotion of colony formation in soft agar medium as compared with parental NIH3T3 cells and the control transfectants [6].
  • Examination of the crystal structure reveals that the NH2-terminal extension in UbcH10 is disordered and that a conserved 3(10)-helix places a lysine residue near the active site [7].

Other interactions of UBE2C

  • To determine whether NKLAM functions as an E3 ligase, we performed coimmunoprecipitation binding assays with ubiquitin conjugates (Ubcs) UbcH7, UbcH8, and UbcH10 [9].


  1. Detection of aberrations of ubiquitin-conjugating enzyme E2C gene (UBE2C) in advanced colon cancer with liver metastases by DNA microarray and two-color FISH. Takahashi, Y., Ishii, Y., Nishida, Y., Ikarashi, M., Nagata, T., Nakamura, T., Yamamori, S., Asai, S. Cancer Genet. Cytogenet. (2006) [Pubmed]
  2. Expression and effect of inhibition of the ubiquitin-conjugating enzyme E2C on esophageal adenocarcinoma. Lin, J., Raoof, D.A., Wang, Z., Lin, M.Y., Thomas, D.G., Greenson, J.K., Giordano, T.J., Orringer, M.B., Chang, A.C., Beer, D.G., Lin, L. Neoplasia (2006) [Pubmed]
  3. Overexpression, genomic amplification and therapeutic potential of inhibiting the UbcH10 ubiquitin conjugase in human carcinomas of diverse anatomic origin. Wagner, K.W., Sapinoso, L.M., El-Rifai, W., Frierson, H.F., Butz, N., Mestan, J., Hofmann, F., Deveraux, Q.L., Hampton, G.M. Oncogene (2004) [Pubmed]
  4. Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. Gabellini, D., Colaluca, I.N., Vodermaier, H.C., Biamonti, G., Giacca, M., Falaschi, A., Riva, S., Peverali, F.A. EMBO J. (2003) [Pubmed]
  5. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Townsley, F.M., Aristarkhov, A., Beck, S., Hershko, A., Ruderman, J.V. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  6. UbcH10 is the cancer-related E2 ubiquitin-conjugating enzyme. Okamoto, Y., Ozaki, T., Miyazaki, K., Aoyama, M., Miyazaki, M., Nakagawara, A. Cancer Res. (2003) [Pubmed]
  7. Structural and functional analysis of the human mitotic-specific ubiquitin-conjugating enzyme, UbcH10. Lin, Y., Hwang, W.C., Basavappa, R. J. Biol. Chem. (2002) [Pubmed]
  8. UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas. Pallante, P., Berlingieri, M.T., Troncone, G., Kruhoffer, M., Orntoft, T.F., Viglietto, G., Caleo, A., Migliaccio, I., Decaussin-Petrucci, M., Santoro, M., Palombini, L., Fusco, A. Br. J. Cancer (2005) [Pubmed]
  9. NK lytic-associated molecule, involved in NK cytotoxic function, is an E3 ligase. Fortier, J.M., Kornbluth, J. J. Immunol. (2006) [Pubmed]
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