Disease relevance of Fdps

• Delayed type hypersensitivity (DTH) is reported as percentage of hind-limb foot pad swelling (%FPS) [1].

High impact information on Fdps

• Protein in vitro translation studies indicated that the extended 5' untranslated region may play a role in regulating the translation of the FPP synthetase polypeptide in rat testis [2].
• Testis-specific transcription initiation sites of rat farnesyl pyrophosphate synthetase mRNA [2].
• The level of CR39 mRNA was increased seven- to ninefold over normal levels by dietary cholestyramine and mevinolin and decreased about fourfold compared with normal levels by cholesterol feeding or administration of mevalonate [3].
• As deduced from the nucleotide sequence of a full-length CR39 cDNA, the rat CR39 polypeptide contained 344 amino acids and had a molecular weight of 39,615 [3].
• The sequence of amino acid residues 173 through 203 in the rat CR39 polypeptide showed that 17 out of 30 matched an active-site peptide of avian liver prenyltransferase [3].

Biological context of Fdps

• Comparison of the hexaprenyl pyrophosphate synthetase amino acid sequence with amino acid sequences from the related enzyme farnesyl pyrophosphate synthetase show the presence of three highly conserved domains [4].
• Testis-specific transcripts of rat farnesyl pyrophosphate synthetase are developmentally regulated and localized to haploid germ cells [5].
• The loci identified in this report may correspond to unique prenyltransferase genes related to FPS or to pseudogenes [6].
• A rat farnesyl pyrophosphate (FPP) synthetase cDNA was used to study the expression of FPP synthetase mRNA levels during spermatogenesis in the rat [5].

Anatomical context of Fdps

• In situ hybridization analysis of adult rat testis sections with an FPP synthetase antisense probe showed that FPP synthetase mRNA levels are greatly enriched in the haploid round spermatidis at stages 7 to 8 of the seminiferous epithelium [5].

Associations of Fdps with chemical compounds

• In testis, an FPP synthetase-related RNA was detected that was larger than the liver FPP synthetase mRNA and was present at very high levels comparable with liver FPP synthetase RNA levels obtained from rats fed diets supplemented with cholestyramine and mevinolin [2].
• In contrast, farnesyl pyrophosphate synthetase mRNA levels and enzyme activity were decreased to similar degrees in response to dietary cholesterol [7].
• The hydrophobicity profiles of the hexaprenyl pyrophosphate synthetase sequence and the farnesyl pyrophosphate synthetase sequence from rat appear similar [4].
• Addition of mevalonolactone to the diet did not further decrease farnesyl pyrophosphate synthetase activity [7].
• Within two of the domains is an aspartate-rich motif found invariantly in the amino acid sequences of farnesyl pyrophosphate synthetase from three species and the hexaprenyl pyrophosphate synthetase amino acid sequence reported here [4].

Other interactions of Fdps

• Dietary cholesterol and mevalonolactone had no effect on mRNA levels for "cellular nucleic acid-binding protein" which has been demonstrated to bind the sterol regulatory elements in the HMG-CoA reductase and farnesyl pyrophosphate synthetase promoters [7].
• RNA blot analysis showed an increase in the level of FPP synthetase transcripts during postnatal development of rat testes [5].

Analytical, diagnostic and therapeutic context of Fdps

• Sequence analysis of testis cDNA clones, together with primer extension and S1 nuclease experiments, indicated that testis FPP synthetase transcripts contain an extended 5' untranslated region [2].
• Differential hybridization and molecular cloning have been used to isolate CR39, a cDNA which hybridizes to a 1.2-kilobase (kb) mRNA in rat liver [3].
• Hypophysectomy resulted in nearly undetectable levels of testicular farnesyl pyrophosphate synthetase mRNA [8].

References