High impact information on ERG20

• Identification of ERG20 reveals that yeast have a limited pool of dimethylallyl pyrophosphate [1].
• Thus, Erg20p appears to affect nonsense suppression by competing with Mod5p for substrate [1].
• Arabidopsis FPS2 was able to rescue the lethal phenotype of an ERG20-disrupted yeast strain [2].
• Disruption of the FPP synthetase gene from a diploid yeast strain, followed by dissection and analysis of tetrads, demonstrates that the gene is an essential, single copy number gene in yeast [3].
• Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae [3].

Biological context of ERG20

• When cloned into the yeast shuttle vector YRp17, the 4.5-kilobase EcoRI fragment directed a 2-3-fold over-expression of FPP synthetase activity in transformed yeast cells [3].
• The gene for FPP synthetase resides on chromosome XI as judged from Southern blots of separated yeast chromosomes [3].

Anatomical context of ERG20

• ERG20 expression was also impaired in pkc1Delta lacking a component of the cell wall integrity signaling pathway [4].

Associations of ERG20 with chemical compounds

• Dolichol formation is examined in three Saccharomyces cerevisiae strains with mutations in the ERG20 gene encoding farnesyl diphosphate synthase (mevalonic acid pathway) and/or the ERG9 gene encoding squalene synthase (sterol synthesis pathway) differing in the amount and chain length of the polyisoprenoids synthesized [5].
• The isolation of the functional ERG20 gene allowed us to show that farnesyl diphosphate synthetase could be a rate limiting enzyme in ergosterol biosynthesis in yeast [6].
• The substrate for Mod5p, dimethylallyl pyrophosphate, is also a substrate for Erg20p that catalyzes an essential step in sterol biosynthesis [1].
• The role of ERG20 gene (encoding yeast farnesyl diphosphate synthase) mutation in long dolichol formation. Molecular modeling of FPP synthase [7].
• The main feature of the mutants blocked in FPP synthetase is their ability to excrete prenyl alcohols, such as geraniol and farnesol [6].

Other interactions of ERG20

• Northern blot analysis indicated a regulatory role of Rsp5p in ERG20 transcription [4].

Analytical, diagnostic and therapeutic context of ERG20

• Southern blot analysis revealed a small FPS gene family in G. biloba [8].

References