Disease relevance of MINA

• We studied, here, the expression of Mina53 in colon cancer to examine its possible role in carcinogenesis [1].
• Mina53 was expressed in all pathological grades of cancer as well as in the adenoma [1].
• Tissue sections of 23 surgical cases of adenocarcinoma and 1 case of adenoma were stained immunohistochemically, and the expression of Mina53 was found to be elevated in all of the adenocarcinomas compared to adjacent nonneoplastic tissues, which showed little staining [1].
• The full-length mdig mRNA was expressed in human lung cancer tissues but was barely detectable in the adjacent normal tissues [2].
• Here we investigated whether mina53 is related to esophageal squamous cell carcinoma (ESCC), a disease with poor prognosis [3].

High impact information on MINA

• Mina53 is a novel Myc target gene that we previously demonstrated to be involved in cell proliferation [1].
• Increased expression of a Myc target gene Mina53 in human colon cancer [1].
• Staining patterns of Ki-67, a biomarker for cell proliferation, were similar to those of Mina53 in most cases, but the percentage of tumor cells stained by anti-Mina53 was higher [1].
• Although anti-Ki-67 antibody strongly stained some well-proliferating nonneoplastic cells including cells in the deeper part of the crypts and in lymphoid germinal centers, antibody to Mina53 rarely stained those cells [1].
• Deeply invading tumors as well as tumors that have invaded lymphatic vessels showed strong immunoreactivity against anti-Mina53 antibody [1].

Biological context of MINA

• A novel myc target gene, mina53, that is involved in cell proliferation [4].
• Specific inhibition of mina53 expression by an RNA interference method severely suppressed cell proliferation [4].
• E-box sites are present in a region proximal to the transcription initiation sites of the mina53 gene [4].
• These results suggest that the mdig gene may be involved in the regulation of cell growth and possibly the development of cancer [2].
• Using a differential display reverse transcription polymerase chain reaction technique and mRNAs of alveolar macrophages from both normal individuals and coal miners, we identified a novel mineral dust-induced gene named mdig, which had not been fully characterized [2].

Anatomical context of MINA

• When expression of c-myc was reduced in human promyelocytic leukemia HL60 cells by phorbol 12-myristate 13-acetate, the expression of mina53 mRNA and protein was reduced [4].
• We generated a specific monoclonal anti-human Mina53 antibody and found that colon tumor cell lines expressed Mina53 highly [1].
• In addition, a number of lung cancer cell lines constitutively express mdig [2].
• The expression of mdig mRNA was detected in alveolar macrophages from coal miners but not from normal subjects [2].
• Interestingly, protein NO52 has been identified as a constituent of free preribosomal particles but is absent from cytoplasmic ribosomes [5].

Associations of MINA with chemical compounds

• 31P NMR and UV-vis spectrometric evidence has revealed an unexpected regioselectivity in the reaction of fenitrothion, 1, an organophosphorus pesticide, with the cetyltrimethylammonium (CTA) surfactants CTAOH and CTAMINA, that incorporate the reactive counterions OH(-) and MINA(-) (the anti-pyruvaldehyde 1-oximate anion) [6].
• The reactive counterion surfactants used were hydroxide anion (HO-) as a normal nucleophile and hydroperoxide anion (HOO-) and the anion of pyruvaldehyde oxime (MINA-) as two alpha-nucleophiles [7].
• Among the compounds tested, interesting structures are those of oximes bearing a thioether substituent [RA 49 (Table 1) and RA 59 (Table 2)] chloro derivative of MINA [RA 55 (Table 2)] and dipyridyl glyoxime methiodide RA 56 (Table 1) [8].
• When formalin-fixed specimens from 52 patients with ESCC were stained immunohistochemically, it was found that Mina53 was highly expressed in 83% of specimens [3].
• Immunolocalization studies revealed that protein NO52 is highly concentrated in the granular component of nucleoli and this characteristic intranuclear distribution is significantly affected by treatment of cells with (i) RNase A, (ii) actinomycin D and (iii) serum starvation [5].

Other interactions of MINA

• When c-Myc in the c-MycER chimeric protein was activated, mina53 mRNA was increased, even in the presence of an inhibitor for protein synthesis [4].

Analytical, diagnostic and therapeutic context of MINA

• The third module, MINA is used, for the start up and control of drug delivery and to synchronize the infusion pumps [9].
• We also found that expression of Mina53 was elevated in colon tumor tissues by immunoblotting analysis [1].
• Expression of Mina53 was investigated by Western blotting in tissue sections from patients with ESCC [3].
• Analyses of immunocomplexes isolated from cellular extracts with an NO52-specific antibody by MALDI mass spectrometry further confirmed the interaction of protein NO52 with various ribosomal proteins as well as with a distinct set of non-ribosomal nucleolar proteins [5].
• To obtain some of the parameters for the equation, a cross-sectional survey was conducted in Mina Nova, a village of gold prospectors in the Amazonian region of Brazil with high rates of bates biting humans [10].

References