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Gene Review

NTG2  -  bifunctional N-glycosylase/AP lyase NTG2

Saccharomyces cerevisiae S288c

Synonyms: Bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase 2, DNA glycoslyase/AP lyase 2, Endonuclease III homolog 2, Endonuclease III-like glycosylase 2, Redoxyendonuclease 2, ...
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Disease relevance of NTG2


High impact information on NTG2

  • Saccharomyces cerevisiae Endo III homologs (NTG1 and NTG2) have been shown to recognize formamidopyrimidine (Fapy) lesions that are derived from purine [2].
  • We conclude that functions of both NTG1 and NTG2 are important for removal of oxidative DNA damage in yeast [3].
  • The yeast genome project has revealed the presence of two genes in Saccharomyces cerevisiae, NTG1 and NTG2, encoding proteins with similarity to endonuclease III [3].
  • We have found that yeast cells simultaneously lacking Ntg1p, Ntg2p, and Apn1p are hyperrecombinogenic (hyper-rec) and exhibit a mutator phenotype but are not sensitive to the oxidizing agents H2O2 and menadione [4].
  • Ntg2 appears to be a nuclear enzyme, whereas Ntg1 was sorted both to the nucleus and to the mitochondria [3].

Chemical compound and disease context of NTG2


Biological context of NTG2

  • The results reported in this study demonstrate that base excision repair (BER) mediated by Ntglp and Ntg2p acts synergistically with NER to repair endogenous or induced lethal and mutagenic oxidative DNA damage in yeast [6].
  • The substrate specificity of Ntg1 p and Ntg2p, and the spectrum of lesions induced by the DNA-damaging agents used, strongly suggest that oxidized DNA bases, presumably oxidized pyrimidines, represent the major targets of this repair pathway [6].
  • Synergism between base excision repair, mediated by the DNA glycosylases Ntg1 and Ntg2, and nucleotide excision repair in the removal of oxidatively damaged DNA bases in Saccharomyces cerevisiae [6].
  • Mutagen sensitivity and enhanced mutagenesis in the rad1 ntg1 ntg2 triple mutant, relative to the other strains tested, were also observed upon exposure to oxidizing agents such as tertbutylhydroperoxide and menadione [6].
  • These biochemical studies strongly support an important biological role for Ntg1p and Ntg2p in the initial processing of abasic sites and maintenance of genomic stability [1].

Anatomical context of NTG2

  • These findings indicate that Ntg2 is, at least in part, responsible for repairing the oxidation products of 8-OHGua in eukaryotic cells [7].

Associations of NTG2 with chemical compounds


Physical interactions of NTG2

  • Using a yeast two-hybrid screen and a GST in vitro transcription and translation assay, the mismatch repair (MMR) protein Mlh1p was demonstrated to interact physically with Ntg2p [10].

Other interactions of NTG2


Analytical, diagnostic and therapeutic context of NTG2


  1. Characterization of AP lyase activities of Saccharomyces cerevisiae Ntg1p and Ntg2p: implications for biological function. Meadows, K.L., Song, B., Doetsch, P.W. Nucleic Acids Res. (2003) [Pubmed]
  2. DNA substrates containing defined oxidative base lesions and their application to study substrate specificities of base excision repair enzymes. Ide, H. Prog. Nucleic Acid Res. Mol. Biol. (2001) [Pubmed]
  3. The Saccharomyces cerevisiae homologues of endonuclease III from Escherichia coli, Ntg1 and Ntg2, are both required for efficient repair of spontaneous and induced oxidative DNA damage in yeast. Alseth, I., Eide, L., Pirovano, M., Rognes, T., Seeberg, E., Bjørås, M. Mol. Cell. Biol. (1999) [Pubmed]
  4. Overlapping specificities of base excision repair, nucleotide excision repair, recombination, and translesion synthesis pathways for DNA base damage in Saccharomyces cerevisiae. Swanson, R.L., Morey, N.J., Doetsch, P.W., Jinks-Robertson, S. Mol. Cell. Biol. (1999) [Pubmed]
  5. Chemical rearrangement and repair pathways of 1,N6-ethenoadenine. Speina, E., Kierzek, A.M., Tudek, B. Mutat. Res. (2003) [Pubmed]
  6. Synergism between base excision repair, mediated by the DNA glycosylases Ntg1 and Ntg2, and nucleotide excision repair in the removal of oxidatively damaged DNA bases in Saccharomyces cerevisiae. Gellon, L., Barbey, R., Auffret van der Kemp, P., Thomas, D., Boiteux, S. Mol. Genet. Genomics (2001) [Pubmed]
  7. Ntg2 of Saccharomyces cerevisiae repairs the oxidation products of 8-hydroxyguanine. Kim, J.E., You, H.J., Choi, J.Y., Doetsch, P.W., Kim, J.S., Chung, M.H. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  8. Substrate specificities of the ntg1 and ntg2 proteins of Saccharomyces cerevisiae for oxidized DNA bases are not identical. Sentürker, S., Auffret van der Kemp, P., You, H.J., Doetsch, P.W., Dizdaroglu, M., Boiteux, S. Nucleic Acids Res. (1998) [Pubmed]
  9. Saccharomyces cerevisiae Ntg1p and Ntg2p: broad specificity N-glycosylases for the repair of oxidative DNA damage in the nucleus and mitochondria. You, H.J., Swanson, R.L., Harrington, C., Corbett, A.H., Jinks-Robertson, S., Sentürker, S., Wallace, S.S., Boiteux, S., Dizdaroglu, M., Doetsch, P.W. Biochemistry (1999) [Pubmed]
  10. Ntg2p, a Saccharomyces cerevisiae DNA N-glycosylase/apurinic or apyrimidinic lyase involved in base excision repair of oxidative DNA damage, interacts with the DNA mismatch repair protein Mlh1p. Identification of a Mlh1p binding motif. Gellon, L., Werner, M., Boiteux, S. J. Biol. Chem. (2002) [Pubmed]
  11. Role of OGG1 and NTG2 in the repair of oxidative DNA damage and mutagenesis induced by hydrogen peroxide in Saccharomyces cerevisiae: relationships with transition metals iron and copper. Melo, R.G., Leitão, A.C., Pádula, M. Yeast (2004) [Pubmed]
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