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Gene Review

PSTPIP1  -  proline-serine-threonine phosphatase...

Homo sapiens

Synonyms: CD2-binding protein 1, CD2BP1, CD2BP1L, CD2BP1S, H-PIP, ...
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Disease relevance of PSTPIP1

  • PSTPIP1, mutated in the syndrome of pyogenic arthritis with pyoderma gangrenosum and acne, interacts both with pyrin and a protein tyrosine phosphatase to regulate innate and adaptive immune responses [1].
  • This CD2BP1-mediated biochemical pathway(s) may function in common inflammatory disorders with apparent etiological overlap, such as rheumatoid arthritis and inflammatory bowel disease [2].
  • HPIP levels are decreased in (V600E)B-RAF-transformed mouse melanocytes, suggesting that HP1beta-mediated suppressive mechanisms correlate with melanoma oncogenesis [3].

High impact information on PSTPIP1


Biological context of PSTPIP1

  • The Y344F PSTPIP1/CD2BP1 mutation, which blocks tyrosine phosphorylation, was associated with a marked reduction in pyrin binding in pervanadate-treated cells [8].
  • Confirmatory PCR and FISH mapping of the der(15) showed loss of chromosome material proximal to the breakpoint on chromosome 15, containing the PSTPIP1 and RCN2 genes [9].
  • A membrane-proximal proline-rich tandem repeat, involved in cytokine production, is recognized by the intracellular CD2 binding protein CD2BP2 [10].
  • Biochemical and RNA-interference studies demonstrated that HPIP influences ERalpha-dependent rapid estrogen signaling by acting as a scaffold protein and recruits Src kinase and the p85 subunit of phosphatidylinositol 3-kinase to a complex with ERalpha, which in turn stimulates AKT and MAPK [11].
  • Here we show that interleukin (IL)-6 regulates the methyltransferase promoter and resulting enzyme activity, which requires transcriptional activation by the Fli-1 transcription factor (Spyropoulos, D. D., Pharr, P. N., Lavenburg, K. R., Jackers, P., Papas, T. S., Ogawa, M., and Watson, D. K. (1998) Mol. Cell. Biol. 15, 5643-5652) [12].

Anatomical context of PSTPIP1

  • Consistent with the hypothesis that these mutations exert a dominant-negative effect on the previously reported activity of pyrin, we found increased IL-1beta production by peripheral blood leukocytes from a clinically active PAPA patient with the A230T PSTPIP1/CD2BP1 mutation and in cell lines transfected with both PAPA-associated mutants [8].
  • Recently, PSTPIP1/CD2BP1 mutations were shown to cause the syndrome of pyogenic arthritis, pyoderma gangrenosum, and acne (PAPA), a dominantly inherited autoinflammatory disorder mediated predominantly by granulocytes [8].
  • Endogenous PSTPIP1/CD2BP1 and pyrin are coexpressed in monocytes and granulocytes and can be coimmunoprecipitated from THP-1 cells [8].
  • These data suggest that the PSTPIP and WASP interaction is regulated by tyrosine phosphorylation of the PSTPIP SH3 domain, and this binding event may control aspects of the actin cytoskeleton [13].
  • We previously observed a defect in cytokinesis and an increase in the tyrosine phosphorylation of PSTPIP in PTP-PEST-deficient fibroblasts [14].

Associations of PSTPIP1 with chemical compounds


Physical interactions of PSTPIP1

  • Mutations in CD2BP1 disrupt binding to PTP PEST and are responsible for PAPA syndrome, an autoinflammatory disorder [2].

Regulatory relationships of PSTPIP1

  • Because of their high binding affinity to pyrin's B-box, PAPA-associated PSTPIP1 mutants were found to be more effective than WT PSTPIP1 in inducing pyrin activation [20].

Other interactions of PSTPIP1


Analytical, diagnostic and therapeutic context of PSTPIP1


  1. The systemic autoinflammatory diseases: inborn errors of the innate immune system. Brydges, S., Kastner, D.L. Curr. Top. Microbiol. Immunol. (2006) [Pubmed]
  2. Mutations in CD2BP1 disrupt binding to PTP PEST and are responsible for PAPA syndrome, an autoinflammatory disorder. Wise, C.A., Gillum, J.D., Seidman, C.E., Lindor, N.M., Veile, R., Bashiardes, S., Lovett, M. Hum. Mol. Genet. (2002) [Pubmed]
  3. Reduced heterochromatin protein 1-beta (HP1beta) expression is correlated with increased invasive activity in human melanoma cells. Nishimura, K., Hirokawa, Y.S., Mizutani, H., Shiraishi, T. Anticancer Res. (2006) [Pubmed]
  4. Cytoskeletal protein PSTPIP1 directs the PEST-type protein tyrosine phosphatase to the c-Abl kinase to mediate Abl dephosphorylation. Cong, F., Spencer, S., Côté, J.F., Wu, Y., Tremblay, M.L., Lasky, L.A., Goff, S.P. Mol. Cell (2000) [Pubmed]
  5. Protein tyrosine phosphatase-PEST regulates focal adhesion disassembly, migration, and cytokinesis in fibroblasts. Angers-Loustau, A., Côté, J.F., Charest, A., Dowbenko, D., Spencer, S., Lasky, L.A., Tremblay, M.L. J. Cell Biol. (1999) [Pubmed]
  6. A cdc15-like adaptor protein (CD2BP1) interacts with the CD2 cytoplasmic domain and regulates CD2-triggered adhesion. Li, J., Nishizawa, K., An, W., Hussey, R.E., Lialios, F.E., Salgia, R., Sunder-Plassmann, R., Reinherz, E.L. EMBO J. (1998) [Pubmed]
  7. PSTPIP: a tyrosine phosphorylated cleavage furrow-associated protein that is a substrate for a PEST tyrosine phosphatase. Spencer, S., Dowbenko, D., Cheng, J., Li, W., Brush, J., Utzig, S., Simanis, V., Lasky, L.A. J. Cell Biol. (1997) [Pubmed]
  8. Pyrin binds the PSTPIP1/CD2BP1 protein, defining familial Mediterranean fever and PAPA syndrome as disorders in the same pathway. Shoham, N.G., Centola, M., Mansfield, E., Hull, K.M., Wood, G., Wise, C.A., Kastner, D.L. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  9. Molecular characterisation of the t(1;15)(p22;q22) translocation in the prostate cancer cell line LNCaP. Strefford, J.C., Lane, T.M., Hill, A., LeRoux, L., Foot, N.J., Shipley, J., Oliver, R.T., Lu, Y.J., Young, B.D. Cytogenet. Genome Res. (2006) [Pubmed]
  10. The GYF domain is a novel structural fold that is involved in lymphoid signaling through proline-rich sequences. Freund, C., Dötsch, V., Nishizawa, K., Reinherz, E.L., Wagner, G. Nat. Struct. Biol. (1999) [Pubmed]
  11. An inherent role of microtubule network in the action of nuclear receptor. Manavathi, B., Acconcia, F., Rayala, S.K., Kumar, R. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
  12. Interleukin-6 regulation of the human DNA methyltransferase (HDNMT) gene in human erythroleukemia cells. Hodge, D.R., Xiao, W., Clausen, P.A., Heidecker, G., Szyf, M., Farrar, W.L. J. Biol. Chem. (2001) [Pubmed]
  13. Tyrosine phosphorylation regulates the SH3-mediated binding of the Wiskott-Aldrich syndrome protein to PSTPIP, a cytoskeletal-associated protein. Wu, Y., Spencer, S.D., Lasky, L.A. J. Biol. Chem. (1998) [Pubmed]
  14. PSTPIP is a substrate of PTP-PEST and serves as a scaffold guiding PTP-PEST toward a specific dephosphorylation of WASP. Côté, J.F., Chung, P.L., Théberge, J.F., Hallé, M., Spencer, S., Lasky, L.A., Tremblay, M.L. J. Biol. Chem. (2002) [Pubmed]
  15. Binding of the intracellular Fas ligand (FasL) domain to the adaptor protein PSTPIP results in a cytoplasmic localization of FasL. Baum, W., Kirkin, V., Fernández, S.B., Pick, R., Lettau, M., Janssen, O., Zörnig, M. J. Biol. Chem. (2005) [Pubmed]
  16. Identification of a novel polyproline recognition site in the cytoskeletal associated protein, proline serine threonine phosphatase interacting protein. Dowbenko, D., Spencer, S., Quan, C., Lasky, L.A. J. Biol. Chem. (1998) [Pubmed]
  17. A coupled proton-transfer and twisting-motion fluorescence probe for lipid bilayers. Mateo, C.R., Douhal, A. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  18. Computer simulations of cyclic and acyclic cholinergic agonists: conformational search and molecular dynamics simulations. McGroddy, K.A., Brady, J.W., Oswald, R.E. Biophys. J. (1994) [Pubmed]
  19. Construction of 3D Layer-Pillared Homoligand Coordination Polymers from a 2D Layered Precursor. Sun, J., Zhou, Y., Fang, Q., Chen, Z., Weng, L., Zhu, G., Qiu, S., Zhao, D. Inorganic chemistry. (2006) [Pubmed]
  20. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Yu, J.W., Fernandes-Alnemri, T., Datta, P., Wu, J., Juliana, C., Solorzano, L., McCormick, M., Zhang, Z., Alnemri, E.S. Mol. Cell (2007) [Pubmed]
  21. Identification of a proline-binding motif regulating CD2-triggered T lymphocyte activation. Nishizawa, K., Freund, C., Li, J., Wagner, G., Reinherz, E.L. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  22. CD2BP1 and CARD15 mutations are not associated with pyoderma gangrenosum in patients with inflammatory bowel disease. Newman, B., Cescon, D., Domenchini, A., Siminovitch, K.A. J. Invest. Dermatol. (2004) [Pubmed]
  23. Analysis of the CD2 and spliceosomal Sm B/B' polyproline-arginine motifs defined by a monoclonal antibody using a phage-displayed random peptide library. Monos, D., Heliopoulos, J., Argyris, E., Cordopatis, P., Zompra, A., Kamoun, M. J. Mol. Recognit. (2006) [Pubmed]
  24. PSTPIP 2, a second tyrosine phosphorylated, cytoskeletal-associated protein that binds a PEST-type protein-tyrosine phosphatase. Wu, Y., Dowbenko, D., Lasky, L.A. J. Biol. Chem. (1998) [Pubmed]
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