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Gene Review

rnhA  -  ribonuclease H

Escherichia coli O157:H7 str. Sakai

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Disease relevance of ECs0210


High impact information on ECs0210


Chemical compound and disease context of ECs0210


Biological context of ECs0210


Anatomical context of ECs0210

  • 30S ribosomal subunits, 70S ribosomes or polysomes from E. coli were subjected to mild ultraviolet irradiation, and the 3'-terminal region of the 16S RNA was excised by 'addressed cleavage' using ribonuclease H in the presence of suitable complementary oligodeoxynucleotides [18].
  • We have previously shown that the overproduction of RNase HI, an enzyme that degrades the RNA portion of an R-loop, can partially compensate for the growth defects because of the absence of topoisomerase I. In this article, we have studied the effects of gyrase reactivation on the physiology of actively growing topA null cells [19].
  • Effect of ribonuclease H from chick embryo on the covalent-linked poly(A)--poly(dA) complementary to poly(dT) template [20].

Associations of ECs0210 with chemical compounds

  • This priming reaction of primase is verified by a number of biochemical methods, including inhibition by modified 3'-phosphate of oligonucleotides and deoxyribonuclease I and ribonuclease H cleavages [21].
  • Identification of single Mn(2+) binding sites required for activation of the mutant proteins of E.coli RNase HI at Glu48 and/or Asp134 by X-ray crystallography [22].
  • 1H NMR studies of deuterated ribonuclease HI selectively labeled with protonated amino acids [12].
  • A Gly residue inserted between alpha-helices B and C appears to relieve unfavorable interactions in the transition state and alternate conformer(s) and represents an important adaptation to adjust conformational changes within RNase H for activity at high temperatures [23].

Other interactions of ECs0210


Analytical, diagnostic and therapeutic context of ECs0210


  1. The kinetic folding intermediate of ribonuclease H resembles the acid molten globule and partially unfolded molecules detected under native conditions. Raschke, T.M., Marqusee, S. Nat. Struct. Biol. (1997) [Pubmed]
  2. Activation/attenuation model for RNase H. A one-metal mechanism with second-metal inhibition. Keck, J.L., Goedken, E.R., Marqusee, S. J. Biol. Chem. (1998) [Pubmed]
  3. On the relationship between protein stability and folding kinetics: a comparative study of the N-terminal domains of RNase HI, E. coli and Bacillus stearothermophilus L9. Sato, S., Xiang, S., Raleigh, D.P. J. Mol. Biol. (2001) [Pubmed]
  4. Expression of a murine leukemia virus Gag-Escherichia coli RNase HI fusion polyprotein significantly inhibits virus spread. VanBrocklin, M., Ferris, A.L., Hughes, S.H., Federspiel, M.J. J. Virol. (1997) [Pubmed]
  5. Stabilization of ribonuclease HI from Thermus thermophilus HB8 by the spontaneous formation of an intramolecular disulfide bond. Hirano, N., Haruki, M., Morikawa, M., Kanaya, S. Biochemistry (1998) [Pubmed]
  6. Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII. Frank, P., Braunshofer-Reiter, C., Wintersberger, U., Grimm, R., Büsen, W. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  7. The rnh gene is essential for growth of Escherichia coli. Kanaya, S., Crouch, R.J. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  8. Co-crystal of Escherichia coli RNase HI with Mn2+ ions reveals two divalent metals bound in the active site. Goedken, E.R., Marqusee, S. J. Biol. Chem. (2001) [Pubmed]
  9. Role of histidine 124 in the catalytic function of ribonuclease HI from Escherichia coli. Oda, Y., Yoshida, M., Kanaya, S. J. Biol. Chem. (1993) [Pubmed]
  10. Importance of the positive charge cluster in Escherichia coli ribonuclease HI for the effective binding of the substrate. Kanaya, S., Katsuda-Nakai, C., Ikehara, M. J. Biol. Chem. (1991) [Pubmed]
  11. Thermostabilization of Escherichia coli ribonuclease HI by replacing left-handed helical Lys95 with Gly or Asn. Kimura, S., Kanaya, S., Nakamura, H. J. Biol. Chem. (1992) [Pubmed]
  12. 1H NMR studies of deuterated ribonuclease HI selectively labeled with protonated amino acids. Oda, Y., Nakamura, H., Yamazaki, T., Nagayama, K., Yoshida, M., Kanaya, S., Ikehara, M. J. Biomol. NMR (1992) [Pubmed]
  13. Thermal stability of Escherichia coli ribonuclease HI and its active site mutants in the presence and absence of the Mg2+ ion. Proposal of a novel catalytic role for Glu48. Kanaya, S., Oobatake, M., Liu, Y. J. Biol. Chem. (1996) [Pubmed]
  14. Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8. Kanaya, S., Itaya, M. J. Biol. Chem. (1992) [Pubmed]
  15. Conformational stabilities of Escherichia coli RNase HI variants with a series of amino acid substitutions at a cavity within the hydrophobic core. Akasako, A., Haruki, M., Oobatake, M., Kanaya, S. J. Biol. Chem. (1997) [Pubmed]
  16. The isolated RNase H domain of murine leukemia virus reverse transcriptase. Retention of activity with concomitant loss of specificity. Zhan, X., Crouch, R.J. J. Biol. Chem. (1997) [Pubmed]
  17. Kinetic and stoichiometric analysis for the binding of Escherichia coli ribonuclease HI to RNA-DNA hybrids using surface plasmon resonance. Haruki, M., Noguchi, E., Kanaya, S., Crouch, R.J. J. Biol. Chem. (1997) [Pubmed]
  18. The topography of the 3'-terminal region of Escherichia coli 16S ribosomal RNA; an intra-RNA cross-linking study. Döring, T., Greuer, B., Brimacombe, R. Nucleic Acids Res. (1992) [Pubmed]
  19. RNase HI overproduction is required for efficient full-length RNA synthesis in the absence of topoisomerase I in Escherichia coli. Baaklini, I., Hraiky, C., Rallu, F., Tse-Dinh, Y.C., Drolet, M. Mol. Microbiol. (2004) [Pubmed]
  20. Effect of ribonuclease H from chick embryo on the covalent-linked poly(A)--poly(dA) complementary to poly(dT) template. Sawai, Y., Kitahara, N., Tsukada, K. FEBS Lett. (1982) [Pubmed]
  21. Synthesis of polyribonucleotide chains from the 3'-hydroxyl terminus of oligodeoxynucleotides by Escherichia coli primase. Sun, W., Godson, G.N. J. Biol. Chem. (1998) [Pubmed]
  22. Identification of single Mn(2+) binding sites required for activation of the mutant proteins of E.coli RNase HI at Glu48 and/or Asp134 by X-ray crystallography. Tsunaka, Y., Takano, K., Matsumura, H., Yamagata, Y., Kanaya, S. J. Mol. Biol. (2005) [Pubmed]
  23. An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H. Butterwick, J.A., Palmer, A.G. Protein Sci. (2006) [Pubmed]
  24. The putative substrate recognition loop of Escherichia coli ribonuclease H is not essential for activity. Keck, J.L., Marqusee, S. J. Biol. Chem. (1996) [Pubmed]
  25. Amyloidogenecity and pitrilysin sensitivity of a lysine-free derivative of amyloid beta-peptide cleaved from a recombinant fusion protein. Cornista, J.C., Koga, Y., Takano, K., Kanaya, S. J. Biotechnol. (2006) [Pubmed]
  26. Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier transform infrared and NMR spectroscopy. Yamasaki, K., Taniguchi, Y., Takeda, N., Nakano, K., Yamasaki, T., Kanaya, S., Oobatake, M. Biochemistry (1998) [Pubmed]
  27. Equilibrium unfolding of Escherichia coli ribonuclease H: characterization of a partially folded state. Dabora, J.M., Marqusee, S. Protein Sci. (1994) [Pubmed]
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