Disease relevance of Rickettsia prowazekii

• We tested sera of nine patients by microimmunofluorescence for antibodies to Rickettsia prowazekii and Rickettsia typhi [1].
• In several respects, therefore, the IFN-gamma-mediated inhibition of scrub typhus rickettsiae in cultured fibroblasts was similar to that reported for Rickettsia prowazekii [2].
• A novel molecular assay was performed at the Centers for Disease Control and Prevention, and Rickettsia prowazekii, the agent of epidemic typhus, was found, rather than R. typhi [3].
• The Rickettsia prowazekii citrate synthase (gltA) gene, previously cloned in Escherichia coli, was localized to a 2.0-kilobase chromosomal fragment [4].
• Phylogenetic analysis of the rompB genes of Rickettsia felis and Rickettsia prowazekii European-human and North American flying-squirrel strains [5].

High impact information on Rickettsia prowazekii

• Cloned mouse interferon-gamma inhibits the growth of Rickettsia prowazekii in cultured mouse fibroblasts [6].
• Obligate intracellular bacteria, such as Rickettsia prowazekii, and higher-plant plastids possess another type of adenylate transporter, which exchanges bacterial or plastidic ADP for ATP from the eukaryotic (host cell) cytoplasm [7].
• We used this new methodology for the determination of membrane topology of the Rickettsia prowazekii ATP/ADP translocase (Tlc) [8].
• Membrane topology of the Rickettsia prowazekii ATP/ADP translocase revealed by novel dual pho-lac reporters [8].
• Cysteine-scanning mutagenesis and thiol modification of the Rickettsia prowazekii ATP/ADP translocase: characterization of TMs IV-VII and IX-XII and their accessibility to the aqueous translocation pathway [9].

Chemical compound and disease context of Rickettsia prowazekii

• Cytokine sensitivity and methylation of lysine in Rickettsia prowazekii EVir and interferon-resistant R. prowazekii strains [10].
• Some characteristics of heavy and light bands of Rickettsia prowazekii on Renografin gradients [11].
• Intact Rickettsia prowazekii was radiolabeled with the glucose oxidase-lactoperoxidase method of iodination [12].
• Purified host-cell-free Rickettsia prowazekii synthesized protein from leucine, lysine, or proline as radioactive precursor [13].
• Cysteine-scanning mutagenesis and thiol modification of the Rickettsia prowazekii ATP/ADP translocase: evidence that TM VIII faces an aqueous channel [14].

Biological context of Rickettsia prowazekii

• Roles of the Fc receptor and respiratory burst in killing of Rickettsia prowazekii by macrophagelike cell lines [15].
• Moreover, the deduced amino acid sequence of the ORF10 product demonstrated a highly hydrophobic profile and showed putative membrane topology similarity to Rickettsia prowazekii ATP/ADP translocase [16].
• Deamination of deoxycytidine nucleotides by the obligate intracytoplasmic bacterium Rickettsia prowazekii [17].
• A member of the typhus group should fulfill at least two of the following four criteria: pairwise nucleotide sequence homologies with rrs, gltA, ompB, and gene D of either Rickettsia typhi or Rickettsia prowazekii of >/=99.4, >/=96.6, >/=92.4, and >/=91.6%, respectively [18].
• Rickettsia prowazekii and ATP/ADP translocase. Analysis of gene fusions encoding beta-galactosidase-ATP/ADP translocase fusion proteins [19].

Anatomical context of Rickettsia prowazekii

• The growth requirement of Rickettsia prowazekii for the amino acids serine and glycine was assessed in both wild-type cell lines and a mutant cell line [20].
• Confocal microscopy of rhodamine phalloidin-stained infected Vero cells revealed the typhus group rickettsiae, Rickettsia prowazekii and Rickettsia typhi, to have no actin tails and short (approximately 1- to 3-micrometer) straight or hooked actin tails, respectively [21].
• In order to identify Rickettsia prowazekii in lice, we developed a panel of 29 representative monoclonal antibodies selected from 187 positive hybridomas made by fusing splenocytes of immunized mice with SP2/0-Ag14 myeloma cells [22].
• Treatment of Rickettsia prowazekii (strain Breinl) grown in chick embryo (CE) yolk sacs with DNase resulted in preparation of host tissue DNA-free rickettsial suspensions suitable for isolation of rickettsial DNA [23].

Gene context of Rickettsia prowazekii

• Macrophage-like RAW264.7 cells are killed by the combination of gamma interferon (IFN-gamma) treatment and infection with Rickettsia prowazekii [24].
• Role of the nitric oxide synthase pathway in inhibition of growth of interferon-sensitive and interferon-resistant Rickettsia prowazekii strains in L929 cells treated with tumor necrosis factor alpha and gamma interferon [25].
• Modified Rickettsia prowazekii strains have been derived from the avirulent Madrid E strain by passage in the lungs of white mice (strain EVir) or by selection for resistance to gamma interferon (IFN-gamma) (strains 427-19 and 87-17) or alpha/beta interferon (IFN-alpha/beta) (strains 83-2P, 60P, 103-2P, and 110-1P) [10].
• The gene (dnaG) coding for DNA primase in the obligate intracellular parasitic bacterium, Rickettsia prowazekii, has been isolated and characterized [26].
• The transcripts of the citrate synthase-encoding gene (gltA) in Rickettsia prowazekii (Rp), an obligate intracellular parasitic bacterium, were analyzed by RNase protection (RP), primer extension (PE) and in vitro transcription assays [27].

Analytical, diagnostic and therapeutic context of Rickettsia prowazekii

• Characterization of the Madrid E strain of Rickettsia prowazekii purified by renografin density gradient centrifugation [28].
• In an in vitro chicken embryo cell culture system, strains of Rickettsia prowazekii, R. mooseri, and R. rickettsii, but not of R. tsutsugamushi, required an atmosphere enriched in CO2 for intracellular growth [29].
• Sequence analysis of the Rickettsia prowazekii gyrA gene [30].
• Five strains of Rickettsia prowazekii different in origin, biological and genetic properties were compared in protein and LPS patterns by the polyacrylamide gel electrophoresis and in antigenic properties by immunoblotting with specific sera of infected white mice [31].

References