The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

dnaG  -  DNA primase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3056, JW3038, dnaP, parB, sdgA
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of dnaG


High impact information on dnaG

  • The rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli [6].
  • Here we present the nucleotide sequence of this parB region spanning 328 bp between 46% and 49% on the plasmid genome. parB is a noncoding region with extensive internal symmetry [7].
  • However, our genetic data indicate that transcription from the gene L-containing operon into parB is required [7].
  • Yeast DNA polymerase--DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity [8].
  • Nucleotide sequence analysis revealed that parB cDNA has homology to glutathione S-transferase (GST; RX:glutathione R-transferase, EC from several sources including plant and animal cells [9].

Chemical compound and disease context of dnaG


Biological context of dnaG


Anatomical context of dnaG

  • The number of flagella per unit cell length decreased sharply after shifting the culture temperature from 30 degrees to 40 degrees C in the following Ts mutants: ftsC108, ftsD1033, ftsE1181, ftsF1141, ftsG29, ftsZ84, parA110, dnaB42, nrdB, and dnaG [15].
  • We have isolated an auxin-regulated cDNA, parB, from the early stage of cultured tobacco mesophyll protoplasts [9].
  • Arabinosylnucleoside 5'-triphosphate inhibits DNA primase of calf thymus [16].

Associations of dnaG with chemical compounds

  • The region coding for the dnaG primase has been identified by NH2-terminal and tryptic peptide amino acid analysis of the dnaG protein [11].
  • Disruption of either sdgA or sdgC blocked salicylate degradation; constructs lacking sdgD accumulated gentisate [17].
  • As GST, and its substrate glutathione, are shown to be related to cell proliferation as well as detoxification of xenobiotics in plant and animal cells, the role of parB is discussed in relation to the induction of proliferative activity in differentiated and nondividing mesophyll protoplasts of tobacco [9].
  • In glycerol gradients, the DNA primase sedimented at a rate corresponding to a molecular mass of about 120 kDa [18].
  • By deletion of the parB locus and using pBluescript KS(+) as an intermediate, pXG40 (2.8 kb), containing unique restriction sites for BamHI, EcoRI, SacI, and KpnI at the ends of the kanamycin resistance gene, was generated [10].

Other interactions of dnaG


Analytical, diagnostic and therapeutic context of dnaG


  1. Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12. Lupski, J.R., Smiley, B.L., Godson, G.N. Mol. Gen. Genet. (1983) [Pubmed]
  2. Host genes involved in the replication of single-stranded DNA phage phiK. Taketo, A. Mol. Gen. Genet. (1976) [Pubmed]
  3. Characterization of the gene coding for the Rickettsia prowazekii DNA primase analogue. Marks, G.L., Wood, D.O. Gene (1993) [Pubmed]
  4. Kid, a small protein of the parD stability system of plasmid R1, is an inhibitor of DNA replication acting at the initiation of DNA synthesis. Ruiz-Echevarría, M.J., Giménez-Gallego, G., Sabariegos-Jareño, R., Díaz-Orejas, R. J. Mol. Biol. (1995) [Pubmed]
  5. A Bacillus subtilis dnaG mutant harbours a mutation in a gene homologous to the dnaN gene of Escherichia coli. Ogasawara, N., Moriya, S., Mazza, G., Yoshikawa, H. Gene (1986) [Pubmed]
  6. The rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli. Lupski, J.R., Godson, G.N. Cell (1984) [Pubmed]
  7. Maintenance of multicopy plasmid Clo DF13 in E. coli cells: evidence for site-specific recombination at parB. Hakkaart, M.J., van den Elzen, P.J., Veltkamp, E., Nijkamp, H.J. Cell (1984) [Pubmed]
  8. Yeast DNA polymerase--DNA primase complex; cloning of PRI 1, a single essential gene related to DNA primase activity. Lucchini, G., Francesconi, S., Foiani, M., Badaracco, G., Plevani, P. EMBO J. (1987) [Pubmed]
  9. parB: an auxin-regulated gene encoding glutathione S-transferase. Takahashi, Y., Nagata, T. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  10. A rolling-circle miniplasmid of Xanthomonas campestris pv. glycines: the nucleotide sequence and its use as a cloning vector. Baldini, R.L., Tahara, S.T., Rosato, Y.B. Plasmid (1999) [Pubmed]
  11. Sequences of the Escherichia coli dnaG primase gene and regulation of its expression. Smiley, B.L., Lupski, J.R., Svec, P.S., McMacken, R., Godson, G.N. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  12. Isolation and characterization of suppressors of two Escherichia coli dnaG mutations, dnaG2903 and parB. Britton, R.A., Lupski, J.R. Genetics (1997) [Pubmed]
  13. Possible new genes as revealed by molecular analysis of a 5-kb Escherichia coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon. Nesin, M., Lupski, J.R., Svec, P., Godson, G.N. Gene (1987) [Pubmed]
  14. Promotion, termination, and anti-termination in the rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli K-12. Lupski, J.R., Ruiz, A.A., Godson, G.N. Mol. Gen. Genet. (1984) [Pubmed]
  15. A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli. Nishimura, A., Hirota, Y. Mol. Gen. Genet. (1989) [Pubmed]
  16. Arabinosylnucleoside 5'-triphosphate inhibits DNA primase of calf thymus. Yoshida, S., Suzuki, R., Masaki, S., Koiwai, O. J. Biochem. (1985) [Pubmed]
  17. Novel pathway of salicylate degradation by Streptomyces sp. strain WA46. Ishiyama, D., Vujaklija, D., Davies, J. Appl. Environ. Microbiol. (2004) [Pubmed]
  18. Purification and properties of a DNA primase from Nicotiana tabacum. Garcia-Maya, M.M., Buck, K.W. Planta (1998) [Pubmed]
  19. The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12. Burton, Z.F., Gross, C.A., Watanabe, K.K., Burgess, R.R. Cell (1983) [Pubmed]
  20. Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein. Nakayama, N., Bond, M.W., Miyajima, A., Kobori, J., Arai, K. J. Biol. Chem. (1987) [Pubmed]
  21. Host factors involved in the growth of microvirid phage alpha 3. Taketo, A. Z. Naturforsch., C, Biosci. (1978) [Pubmed]
  22. Bacillus subtilis dnaE encodes a protein homologous to DNA primase of Escherichia coli. Wang, L.F., Price, C.W., Doi, R.H. J. Biol. Chem. (1985) [Pubmed]
  23. An over-expression plasmid for Escherichia coli primase. Godson, G.N. Gene (1991) [Pubmed]
WikiGenes - Universities