Disease relevance of Phosphinidene

• CONCLUSIONS: Risk of early neurological deterioration and of 3-month death was severely increased after PH2, indicating that large hematoma is the only type of hemorrhagic transformation that may alter the clinical course of ischemic stroke [1].
• CCl4 (100 microliters kg-1)-induced histopathological alterations in CD-pretreated rats were significantly decreased in rats 2 days post-PH (PH2) as compared to SH rats or rats 7 days post-PH (PH7), indicating that amplification of CCl4 toxicity is significantly reduced when there is a greater regenerative activity [2].
• The primary hyperoxalurias (PH1 and PH2) are rare defects of oxalate overproduction [3].
• We conclude that all children with nephrolithiasis secondary to hyperoxaluria should have urinary glycerate measured, as PH2 may be more prevalent than currently estimated [3].
• Stool specimens were examined for Oxalobacter formigenes in HyOx not related to PH type 1 or 2 (PH1, PH2) and in controls [4].

High impact information on Phosphinidene

• Hydrogen consumption varied directly with PH2, and methanogenic feces consumed H2 far more rapidly than did nonmethanogenic feces [5].
• In contrast, the FYVE-finger and PH2 domains do not appear to direct the localization of Fgd1 or the activation of Cdc42 [6].
• By transient transfections in beta and non-beta cells, we show that mainly PH1 and PH2 preferentially confer beta-cell-specific activation on a heterologous promoter [7].
• We obtained here a monoclonal antibody named PH2 that inhibited macrophage phagocytosis of late apoptotic or necrotic cells, but not of early apoptotic cells [8].
• These results suggest that the putative PH2 antigen is a novel phagocytosis marker that translocates to the cell surface at late stages of apoptosis, resulting in maximal recognition and engulfment by macrophages [8].

Biological context of Phosphinidene

• This study examines whether the course of adrenarche [rise of serum dehydroepiandrosterone sulfate (DHEAS)] and pubarche (Tanner stage PH2) is independent from ovarian function [9].
• The Ras-GRF1 exchange factor molecule contains in addition to the catalytic domain two pleckstrin homology (PH1 and PH2), one IQ and one Dbl homology (DH) domains [10].
• Reactivity of Pd(0) complexes with the phosphino ylide [Ph2PCH2PPh2=C(H)C(O)Me]. Molecular structure of [Pd(PPh2CHPPh2C(H)C(O)Me)2] [11].
• Initial experiments established that peak regeneration occurs at 2 days post-PH (PH2) and liver regeneration phases out by 7 days post-PH (PH7) [12].
• Formation of a bowl-shaped, pentacyclic phosphonium cage by methylation of a nucleophilic phosphinidene [13].

Anatomical context of Phosphinidene

• Feces incubated at high and intermediate PH2 had a net H2 production of only 1/900 and 1/64 of absolute production [5].
• Moreover, PH2 bound to apoptotic cells at late stages more efficiently than to those at early stages, and it did not bind to normal cells unless their plasma membrane was permeabilized [8].
• Although DH and PH1 show exchange activity, FAB, in addition to DH and PH1, is required for the formation of microspikes, whereas FYVE and PH2, in addition to DH and PH1, are required for the formation of lamellipodia [14].
• In this paper we outline diagnostic criteria for identification of PH2 in two patients, one with maintained renal function and one with ESRF on CPD, based on the use of a novel HPLC assay of L-glycerate in different body fluids [15].
• Between PH2 and PH112 hair-cell numbers increase by a further 36%, to 36,650/macula [16].

Associations of Phosphinidene with other chemical compounds

• Such behavior was also shown by recombinant AH1, PH2, and PH4 proteins after expression in Pichia pastoris [17].
• We previously isolated a monoclonal antibody named PH2 that inhibits phosphatidylserine-mediated phagocytosis of apoptotic cells by macrophages [18].
• The bridging phosphinidene complexes [Mn2(CO)8(micro-PNiPr2)] and [Co2(CO)4(micro-dppm)(micro-PNR2)](NR2=NiPr2, TMP) react with heterocumulenes RN3, CH2N2 and Ph2C=N=N to form complexes with micro-eta1,eta2-aminophosphaimine, micro-eta1,eta2-aminophosphaalkene and micro-eta1,eta2-aminophosphadiphenylmethylazaimine ligands, respectively [19].
• A trend towards early mean age of attainment of B2 and B3 was found among girls of European-American-Israeli ethnic origin and towards early mean age of attainment of PH2 and PH3 among girls of Near Eastern origin [20].

Gene context of Phosphinidene

• CONCLUSION: These results suggest that frabin recognizes a specific actin structure(s) through FAB and a specific membrane structure(s) through FAB and the region containing DH, PH1, FYVE and PH2 [14].
• The other covers PHS1 and PH2 that localizes to filopodia, filopodial puncta and the sites of intercellular adhesion in a Cdc42-dependent manner [21].
• The GRHPR genes from nineteen unrelated patients with PH2 were analysed for mutations using a combination of PCR-SSCP and sequence analysis of genomic and cDNA [22].
• While genetic analysis of PH2 is still at a relatively early stage, the AGXT gene defective in the Type 1 disorder is well characterized, and a number of mutations have been identified [23].
• On the basis of a comprehensive survey of possible structures, energetics, and simulated STM images, three prominent STM features are assigned to structures containing surface bound PH2, PH, and P, respectively [24].

Analytical, diagnostic and therapeutic context of Phosphinidene

• Magnetic circular dichroism and absorption spectra of phosphinidene in noble-gas matrices [25].
• MATERIAL AND METHODS: Type III partial denture casting investments (Wirovest, Wiroplus, Biosint, PH2) were tested [26].
• Genomic DNA studies have identified those genetic defects of PH1 and PH2 that allow a precise early diagnosis [27].
• However, there is little doubt of the value of genetic methods (mutation and linkage analysis) for diagnosing PH1 (and eventually PH2) in other family members and for prenatal diagnosis and carrier testing [23].

References