Disease relevance of beta-Aspartylglycine

• The effect on the colonisation resistance was measured by the concentration of resistant bacteria (either endogenous enterococci or an exogenous strain of Enterobacter cloacae) as well as by the relative caecal weight and the appearance of beta-aspartylglycine in the caecal contents [1].
• Identification of beta-aspartylglycine in uremic serum and its toxicity [2].
• The impact on the indigenous (mostly anaerobic) flora was measured in four ways: (i) beta-aspartylglycine content; (ii) volatile fatty acid pattern; (iii) yeast overgrowth and (iv) Streptococcus faecalis decrease [3].
• The mice given more than 1,0 g/kg body weight of beta-aspartylglycine showed behavioral alterations: low response to the stimuli and low activity, and some mice died by the injection of 4.0 g/kg body weight of the peptide [2].
• The toxicity of beta-aspartylglycine in mice with acute renal failure induced by uranyl acetate was investigated [2].

High impact information on beta-Aspartylglycine

• Confirmation of D-aspartic acid in the novel dipeptide beta-aspartylglycine isolated from tissue extract of Aplysia kurodai [4].
• Synthesis of the novel dipeptide beta-aspartylglycine by Aplysia ganglia [5].
• In contrast with native lysozyme, the limited hydrolysis of this abnormal tryptic peptide of Ile-98-Arg-112 in 6 N HCl at 110 degrees C gave a considerable amount of beta-aspartylglycine [6].
• Approximately 20% of the tissue radioactivity was recovered in the peptides beta-aspartylglycine and glutathione after incubation with [14C]glycine [5].
• Resistance of the gastrointestinal tract against colonization by microorganisms from the environment (so-called colonization resistance) remained unimpaired, as measured by the absence of beta-aspartylglycine in the stool [7].

Anatomical context of beta-Aspartylglycine

• Relative caecal weights, the ratio of secondary to primary bile acids, the presence of filamentous segmented bacteria in the small intestine and faecal beta-aspartylglycine were normal 5 days after direct contact and 15 days after indirect contact [8].
• Bodyweight and the following intestinal parameters were determined for the 3 groups: colonization resistance to E. coli, relative caecal weight, villus:crypt ratio (ileum), beta-aspartylglycine (faeces), volatile fatty acids (caecum), and bile acids (faeces) [9].

Associations of beta-Aspartylglycine with other chemical compounds

• The biochemical parameters studied were degradation of mucin, inactivation of tryptic activity, conversion of cholesterol to coprostanol and of bilirubin to urobilinogen, degradation of beta-aspartylglycine, and formation of short-chain fatty acids [10].
• The effect of enteral nutrition (EN) on the following seven intestinal microflora-associated characteristics (MACs) was studied: formation of urobilinogen, coprostanol and deoxycholic acid, degradation of mucin and beta-aspartylglycine, faecal tryptic activity, and production of short-chain fatty acids (SCFAs) [11].
• Additionally, four parameters (production of SCFA. conversion of cholesterol to coprostanol, inactivation of trypsin, degradation of beta-aspartylglycine) were investigated in faecal samples from germ-free minipigs [12].

Gene context of beta-Aspartylglycine

• Mucin and beta-aspartylglycine were totally degraded, as occurs similarly in other animal species [13].
• The MACs studied were degradation of mucin, conversion of cholesterol to coprostanol and bilirubin to urobilinogens, inactivation of tryptic activity, degradation of beta-aspartylglycine and excretion of short-chain fatty acids [13].

Analytical, diagnostic and therapeutic context of beta-Aspartylglycine

• This method was used to determine beta-aspartylpeptidase activity in faecal supernatants of healthy human volunteers and antibiotic-treated patients with beta-aspartylglycine as substrate [14].
• This study describes and compares four methods--(1) dansylation with thin-layer chromatography, (2) ion-exchange chromatography, (3) thin-layer electrophoresis, (4) high-voltage paper electrophoresis--to determine the concentration of beta-aspartylglycine in fecal supernatants of leukemic patients treated with antimicrobial agents [15].
• This substance was isolated from hemodialysate by the methods of ion-exchange chromatography, gel-filtration and paper electrophoresis, and identified as beta-aspartylglycine by amino acid analysis, N-terminal amino acid determination and comparison with authentic sample synthesized in this laboratory [2].
• The results indicate that monitoring the normalization (association) process can be accomplished in several ways, but the level of colonization-resistance is most easily measured by high-voltage paper electrophoresis of faecal supernatants to determine the concentration of beta-aspartylglycine [16].

References