Disease relevance of L-aspartate

• Aslfm, the D-Aspartate Ligase Responsible for the Addition of D-Aspartic Acid onto the Peptidoglycan Precursor of Enterococcus faecium [1].
• In this report, we present high-resolution crystal structures of the complexes of Erwinia chrysanthemi L-asparaginase (ErA) with the products of such reactions that also can serve as substrates, namely L-glutamic acid (L-Glu), D-aspartic acid (D-Asp), and succinic acid (Suc) [2].
• Aggravation by morphine and D-aspartic acid of pyelonephritis induced by i.v. inoculation of Staphylococcus aureus in rats [3].
• Structure of the O-polysaccharide of Proteus mirabilis O38 containing 2-acetamidoethyl phosphate and N-linked D-aspartic acid [4].
• Stimulation of growth of Mycobacterium lepraemurium in cell-free liquid medium by DL-aspartic acid [5].

Psychiatry related information on L-aspartate

• Intensification and attenuation of morphine dependence by D-aspartic acid and PLG [6].
• In this report we present evidence for the presence of free D-aspartic acid (D-Asp) and D-alanine (D-Ala) in the white and gray matter of normal human brains and brains of individuals with Alzheimer's disease [7].

High impact information on L-aspartate

• We report here the activity of 3'-azido-3'-deoxythymidine, 2',3'-dideoxyinosine, 2',3'-dideoxycytidine, and two hydroxamates, D-aspartic acid beta-hydroxamate and hydroxycarbamate (hydroxyurea), alone and in various combinations, in an in vitro model based on resting lymphocytes [8].
• Our results indicate that the use of aspartame to sweeten neutral pH foods and beverages that are then heated at elevated temperature could generate D-aspartic acid and D-phenylalanine [9].
• This is demonstrated by treatment of proteolytically derived aspartic acid beta-[3H]methyl ester with L- and D-amino-acid oxidases and by the resolution of diastereomeric L-leucyl dipeptides containing either L- or D-aspartic acid beta-methyl ester by ion-exchange chromatography [10].
• d-Aspartic acid (d-Asp), an endogenous amino acid present in vertebrates and invertebrates, plays an important role in the neuroendocrine system, as well as in the development of the nervous system [11].
• D-Aspartic acid was used as a specific substrate to evaluate the effects of dipolar amino acids on the high affinity anionic amino acid transport system X-AG in rabbit jejunal brush-border membrane vesicles [12].

Chemical compound and disease context of L-aspartate

• Antagonistic effect of L-aspartic acid on decrease in body weight, and food and fluid intake, and naloxone reversible rectal temperature depression caused by D-aspartic acid [13].
• L-Cysteine hydrochloride exhibited absolute toxicity against both the test pathogens while DL-aspartic acid was found active against Microsporum gypseum only [14].
• Evaluation of D-amino acid levels in rat by gas chromatography-selected ion monitoring mass spectrometry: no evidence for subacute toxicity of orally fed D-proline and D-aspartic acid [15].

Biological context of L-aspartate

• We have measured the rate of accumulation of amino acid residues in human erythrocyte membrane and cytosolic proteins which give D-aspartic acid upon acid hydrolysis [16].
• Substrate translocation kinetics of excitatory amino acid carrier 1 probed with laser-pulse photolysis of a new photolabile precursor of D-aspartic acid [17].
• Inhibition of aspartoacylase by glycyl-L-aspartate and amino derivatives of D-aspartic acid suggests that the carbon backbone of the substrate is primarily involved in its interaction with the active site and that a blocked amino group is essential for the catalytic activity of aspartoacylase [18].
• Secretion of D-aspartic acid by the rat testis and its role in endocrinology of the testis and spermatogenesis [19].
• These results indicate that the D-aspartic acid contents of the molars of SAMR1/Iw and SAMP2/Iw increase with age in a different fashion and suggest that the fashion was determined by the body temperature, but not by the senescence-accelerated age [20].

Anatomical context of L-aspartate

• Age-dependent accumulation of protein residues which can be hydrolyzed to D-aspartic acid in human erythrocytes [16].
• 2-Chloroadenosine failed to suppress the evoked release of glutamic acid, measured in these same superfusates, unless the mossy fiber synaptosomes were pretreated with D-aspartic acid to deplete the cytosolic, Ca2+-independent, pool of this acidic amino acid [21].
• The brain efflux index method has been used to clarify the mechanism of efflux transport of acidic amino acids such as L-aspartic acid (L-Asp), L-glutamic acid (L-Glu), and D-aspartic acid (D-Asp) across the blood-brain barrier (BBB) [22].
• Free D-aspartic acid and NMDA are present in the mammalian central nervous system and endocrine glands at significant concentrations, but their physiological role is still matter of debate [23].
• The accumulation with age of D-aspartic acid in primary and secondary human dentin was determined [24].

Associations of L-aspartate with other chemical compounds

• At pH 6, increasing L-phenylalanine concentrations caused a saturable activation of 0.05 mM D-aspartic acid uptake (Ka = 2.4 mM), and a saturating concentration of effector increased the Vmax of transport (2.6-fold) without any significant effect on the Km [12].
• It was shown by optical-rotatory-dispersion spectrum and optical-resolution h.p.l.c. analysis that this compound consisted of equimolar amounts of D-aspartic acid and glycine [25].
• The crystallization of DL-aspartic acid beneath nylon 6 spread films has been studied for 150% supersaturated systems using neutron reflectivity and external reflection FTIR [26].
• d-Aspartic acid and nitric oxide as regulators of androgen production in boar testis [27].
• Involvement of D-aspartic acid in the synthesis of testosterone in rat testes [28].

Gene context of L-aspartate

• D-aspartic acid in purified myelin and myelin basic protein [29].
• The lens enzyme appears to be similar to that found in human erythrocytes, which specifically recognizes and modifies D-aspartic acid residues in aged proteins in a postulated degradative or racemization-repair pathway (McFadden, P.N., and Clarke, S. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 2460-2464) [30].
• Endogenous testicular D-aspartic acid regulates gonadal aromatase activity in boar [31].
• In a previous report (Hamase, K. et al., Biochim Biophys Acta 1134: 214-222 (1997)), we showed that the rat pineal gland contains D-leucine (D-Leu) as well as D-aspartic acid (D-Asp) [32].
• Vasopressin release by D-aspartic acid, morphine and prolyl-leucyl-glycinamide (PLG) in DI Brattleboro rats [33].

Analytical, diagnostic and therapeutic context of L-aspartate

• Initial rates of Na(+)-dependent L-glutamic and D-aspartic acid uptake were determined at various substrate concentrations using a fast sampling, rapid filtration apparatus, and the resulting data were analyzed by nonlinear computer fitting to various transport models [34].
• Continuous on-line derivatization and selective separation of D-aspartic acid by a capillary electrophoresis system with a continuous sample introduction interface [35].
• External reflection FTIR studies revealed very weak bands attributable to DL-aspartic acid in surface regions devoid of visible crystals, in agreement with the neutron reflectivity studies [26].
• Specific interaction was detected between tRNA and its cognate L-amino acid by circular dichroism (CD) and fluorescence spectroscopy; fluorescence spectral change with saturation was observed when tRNAAsp was titrated only with L-aspartic acid, but not with D-aspartic acid, L- and D-glutamic acids [36].
• Morphine and D-aspartic acid, when combined at their nonanalgesic doses, led to significant analgesia [37].

References