Disease relevance of Diacetatodioxouranium

• The preservation of the spherical shape of the virus was obtained by glutaraldehyde fixation dehydration in alcholic solutions of uranyl acetate, and critical point drying [1].
• Possible involvement of myofibroblasts in cellular recovery of uranyl acetate-induced acute renal failure in rats [2].
• Micrographs of one of the views of the large Escherichia coli subunit negatively stained with uranyl acetate were computer averaged using two techniques: the maximum-likelihood method and the correlation method [3].
• The study was performed to elucidate the progression and regression of superficial and inner glomerular alterations in uranyl acetate-induced renal failure in rabbits [4].
• We have determined the ultrastructure of 5-hydroxytryptamine3 (5-HT3) serotonin receptors purified from NG108-15 mouse neuroblastoma x rat glioma cells by electron microscopic examination of receptor particles embedded in uranyl acetate stain and metal replicas of rapidly frozen receptors [5].

Psychiatry related information on Diacetatodioxouranium

• The antigenicity of the 'prion' protein amyloid fibrils was shown to be preserved after glutaraldehyde/OsO4 fixation in uranyl acetate-stained brain tissue blocks from patients with Gerstmann-Sträussler syndrome (GSS) and from mice infected with Creutzfeldt-Jakob disease (CJD) [6].

High impact information on Diacetatodioxouranium

• Using A-filaments prepared from rat psoas muscle we have now found it possible to induce clear fraying of these filaments into subfilaments, by exposure of the preparation to very low ionic strength before contrasting with uranyl acetate [7].
• When ultrathin frozen sections of chicken cardiac muscle were osmicated, dehydrated in ethanol, embedded in ethyl cellulose, and stained with acidic uranyl acetate, filaments of 10-12 nm width were visualized in wide interfibrillar spaces [8].
• Micrographs of mouse liver gap junctions, isolated with detergents, and negatively stained with uranyl acetate, have been recorded by low-irradiation methods [9].
• For a 500-1,000-A biological tissue section fixed with OsO4 and stained with uranyl acetate, we obtained resolution (half distance, HD) values of approximately 800 +/- 120 A using Ilford L4 emulsion and 500 +/- 70 A using a Kodak NTE-type emulsion [10].
• Negative staining with uranyl acetate revealed no differences in gametic and vegetative flagellar surfaces; both had flagellar membranes, flagellar sheaths, and similar numbers and distributions of mastigonemes [11].

Chemical compound and disease context of Diacetatodioxouranium

• Mycophenolate mofetil inhibits regenerative repair in uranyl acetate-induced acute renal failure by reduced interstitial cellular response [12].
• Human eccrine sweat glands, including those expressing the gene for cystic fibrosis, were stained sequentially en bloc with uranyl acetate, lead citrate and copper sulfate, and osmium tetroxide for the ultrastructural demonstration of tubulocisternal endoplasmic reticulum (TER) [13].
• Antibody molecules bound to rotavirus particles were observed clearly only with phosphotungstic acid staining and not with uranyl acetate [14].
• Such changes, however, were not evident in specimens previously exposed to uranyl acetate during ethanol dehydration [15].
• To evaluate the developmental toxicity of uranium, 5 groups of 20 pregnant Swiss mice were given by gavage daily doses of 0, 5, 10, 25 and 50 mg/kg of uranyl acetate dihydrate on gestational days 6-15 [16].

Biological context of Diacetatodioxouranium

• We examined the serial changes in tubular damage and origin and kinetics of regenerating cells in uranyl acetate-induced acute renal failure, with a special emphasis on interstitial myofibroblasts [2].
• (b) The enhansive and suppressive effects of 8-anilino-1-naphthalenesulfonate, NiCl2, and uranyl acetate on the responses to anodal current were similar to those on the responses to chemical stimulation [17].
• Role of apoptosis in uranyl acetate-induced acute renal failure and acquired resistance to uranyl acetate [18].
• The day following the i.v. injection of uranyl acetate (2 mg/kg of body wt), renal blood flow (RBF) and clearance of creatinine (Ccr) decreased to approximately 60 and 20% of controls, respectively [19].
• Trypsin-treated chromosomes stained with uranyl acetate alone also showed clear G-bands under TEM [20].

Anatomical context of Diacetatodioxouranium

• Spread chromatin fibers and isolated chromatin fragments prepared from chicken erythrocyte nuclei were stained with dilute aqueous uranyl acetate [21].
• ImmunoGold (Janssen) labeling using chaperonin antibodies indicated that many chaperonins are associated with insoluble cellular structures and these structures appear to be filamentous in some areas, although they could not be uranyl-acetate-stained [22].
• We recently reported that transient appearance of interstitial myofibroblasts and infiltrating macrophages might play a role in cellular recovery in uranyl acetate (UA)-induced acute renal failure (ARF) [12].
• When sections from the cerebral cortex of 5 normal rats and 2 normal monkeys were stained with saturated aqueous uranyl acetate (without block staining) and examined with the electron microscope, all 7 animal brains showed abundant membrane lamellation between apposed neuronal and neuroglial processes [23].
• Actin microfilaments form a distinct laminar core within the ridge; they are seen as a sparse, disordered meshwork in cytoskeletons prepared by conventional methods using uranyl acetate negative staining [10] [24].

Associations of Diacetatodioxouranium with other chemical compounds

• In vitro experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although not as rapidly as U(VI) present as uranyl acetate [25].
• The two most informative density maps were from specimens embedded in ice and from negative staining in a 1:1 mixture of glucose and uranyl acetate [26].
• Electron microscopy was performed on thin sections stained with uranyl acetate and lead citrate [27].
• Significant cell volume changes occurred after osmium (16 per cent swelling), postosmium wash (10 per cent swelling), and uranyl acetate (25 per cent shrinkage) [28].
• Spinal cord motoneuron neuropil (cervical and lumbar enlargements) has been studied at the ultrastructural level after fixation with glutaraldehyde and staining with uranyl acetate and lead citrate [29].

Gene context of Diacetatodioxouranium

• Freeze-substitution of tissue was performed with anhydrous methanol and 0.5% uranyl acetate at -90 degrees C. Semi-thin Lowicryl sections were used for light microscopic visualization of B-50 in the ventromedial mesencephalic central gray substance [30].
• Immunochemical staining of renal sections for Hsp25 and Hsp70i revealed that these proteins were prominently expressed in tubular epithelial cells in uranyl acetate pretreated animals [31].
• We have studied here the association of purified bovine SP-A with lipid vesicle bilayers in vitro with negative staining with uranyl acetate and transmission electron microscopy [32].
• Both free and thermolysin-bound ovostatin preparations were negatively stained with uranyl acetate, a series of three pictures were taken at 10 degrees specimen tilt intervals (+10 degrees, 0 degrees, and -10 degrees), and images of the inhibitor molecules were observed in three dimensions [33].
• The three-dimensional structure of PhoE porin from Escherichia coli, negatively stained with uranyl acetate, has been determined by electron crystallographic techniques to a resolution of about 18 A. The structure shows that PhoE porin consists of trimeric stain-filled channels as the basic unit [34].

Analytical, diagnostic and therapeutic context of Diacetatodioxouranium

• The grids were then stained with uranyl acetate and the display of fibrous structures recorded by electron microscopy [35].
• The fragments so generated were fractionated by gel filtration chromatography and visualized by negative staining with uranyl acetate on fenestrated carbon films [36].
• Acute renal failure was induced in rats by intravenous injection of uranyl acetate (5 mg/kg) [2].
• The physical state of actin in nerve ending preparations and its relationship to the membranes was studied at the ultrastructural level by negative staining with uranyl acetate before and after treatment with muscle heaving meromyosin (HMM) [37].
• We developed a new procedure, which entails application of high-pressure freezing followed by freeze-substitution with acetone containing uranyl acetate, followed by low temperature embedding in HM20 [38].

References