Disease relevance of ptsH

• The nucleotide sequence of ptsH gene from Klebsiella pneumoniae [1].
• Isolation and investigation of the Escherichia coli mutant with the deletion in the ptsH gene [2].
• Suppression of the ptsH mutation in Escherichia coli and Salmonella typhimurium by a DNA fragment from Lactobacillus casei [3].
• Analysis of a ptsH homologue from Streptomyces coelicolor A3(2) [4].
• The gene (ptsH) for the phosphocarrier protein, HPr, of the phosphoenolpyruvate:sugar phosphotransferase system from Mycoplasma capricolum was previously cloned and sequenced [5].

High impact information on ptsH

• The phosphoryl groups are donated by the phosphocarrier protein HPr [6].
• Promoter-reporter gene fusion studies identified two CRP.cAMP-dependent promoters (the previously identified P1 and another promoter, P0) upstream of ptsH [7].
• Third, growth of the ptsH mutants on fructose relieved PTS-mediated repression; after growth on fructose, but not on lactate, the mutants could grow for several generations on non-PTS sugars [8].
• Growth on other PTS sugars, besides glucose, also induces ptsH and ptsG expression, suggesting that the target of Mlc regulation is the PTS [9].
• An open reading frame overlapping the main part of ptsH in the opposite sense was shown to be expressed in vivo, using protein fusions with beta-galactosidase [10].

Chemical compound and disease context of ptsH

• A DNA fragment from Lactobacillus casei that restores growth to Escherichia coli and Salmonella typhimurium ptsH mutants on glucose and other substrates of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) has been isolated [3].
• An Escherichia coli strain, LBG1605, carrying a mutated ptsH gene (encoding HPr (His-containing protein) of the bacterial phosphotransferase system (PTS)) and complemented for PTS activity with the ptsH of Staphylococcus carnosus, exhibited reduced mannitol fermentation activity when transformed with a plasmid bearing this 3574-bp Bs fragment [11].
• Effect of ptsI and ptsH mutations on initiation of transcription of the Escherichia coli lactose operon [12].
• HPr protein, the ptsH gene product, of the phosphoenolpyruvate:sugar phosphotransferase system of Pediococcus halophilus was purified to homogeneity using heat and acid treatments, DEAE-Sepharose CL-6B and hydroxyapatite column chromatographies [13].
• Sequence and expression of the genes for HPr (ptsH) and enzyme I (ptsI) of the phosphoenolpyruvate-dependent phosphotransferase transport system from Streptococcus mutans [14].

Biological context of ptsH

• In M. capricolum, ptsH and ptsI reside on 2 distinct operons at separate loci on the chromosome (Zhu PP, Reizer J, Reizer A, Peterkofsky A, 1993, J Biol Chem 268:26531-26540) [15].
• 44 on the E. coli genome, was transferred by phage-mediated transduction to appropriate recipients that lack HPr (ptsH), or Enzyme IIman (ptsM), or neither [16].
• Experiments indicated that the reversion of the ptsH phenotype was due to a titration of the Cra protein, which allowed the constitutive expression of the fructose operon [3].
• As for E. coli, a B. subtilis ptsH null mutant is severely deficient in chemotaxis toward most PTS carbohydrates [17].
• Upstream, the 264-bp open reading frame of the ptsH gene is flanked by a putative S. carnosus promoter structure and a putative ptsI gene downstream suggesting that ptsH gene is the first gene in the PTS operon of S. carnosus [18].

Associations of ptsH with chemical compounds

• These mutations suppressed the defects in a ptsH mutant which lacks HPr, one of the enzymes of the phosphoenolpyruvate: carbohydrate phosphotransferase system [19].
• The intracellular content of cyclic AMP in the ptsIH mutant was severely diminished, while the ptsH bacteria contain the same amounts of this nucleotide as the wild-type cells [12].
• The new sampling method (essential dynamics sampling) was applied to the histidine-containing phosphocarrier protein HPr [20].

References