Disease relevance of ANAPC10

• BACKGROUND: We previously reported differential expression of the growth suppressor, deleted in oral cancer-1 (DOC-1), in microsatellite-unstable (MSI+) versus microsatellite-stable colorectal cancer (CRC) cell lines [1].

High impact information on ANAPC10

• We further provide biochemical evidence that the C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains multiple tetratrico peptide repeats [2].
• Unexpectedly, this structure is highly similar to ligand-binding domains of several bacterial and eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet unidentified ligand [2].
• APC activation by CDH1 depends on a conserved C-terminal motif that is also found in CDC20 and APC10 [3].
• We have further identified an APC10 homology region, which we propose to call the DOC domain, in several protein sequences that also contain either cullin or HECT domains [4].
• Here, we show that Doc1/Apc10 is a subunit of the yeast APC throughout the cell cycle [4].

Biological context of ANAPC10

• Identification of human APC10/Doc1 as a subunit of anaphase promoting complex [5].
• Further, it was found that APC10/Doc1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in midbody in telophase and cytokinesis [5].
• CONCLUSION: Doxazosin induces the apoptosis of cardiomyocytes via the ER pathway, with increased production of C/EBP beta, GADD153 and DOC-1 [6].
• However, the serine and threonine phosphorylation of the EGFR of APC-10 cells was consistently 2-3-fold lower than that seen in PC-10 cells [7].
• The APC-10 cells exhibited a unique response to EGF, and no concentration of EGF tested could produce cell death [7].

Anatomical context of ANAPC10

• Further subtraction of normal endothelium, bone marrow, white blood cell and other normal tissue transcripts resulted in just three gene transcripts, ANAPC10, PLXDC1(TEM7), and CYP27B1, that are highly specific to GBM-ECs [8].
• METHODS: SW48 is an MSI+ CRC cell line that does not constitutively express DOC-1 [1].

Associations of ANAPC10 with chemical compounds

• From the total of 50 subjects, a new relationship was developed: Log10 (APC10) = 0.68 log10 (HPC20 X SS) (r = 0.82) from which 46 of 50 (92%) of allergen PC20 values fall within 2 doubling concentrations of the regression line (and all within 3) [9].

Other interactions of ANAPC10

• In addition, the protein levels of HEF1 are subjected to the regulation of overexpressed APC10 and CDH1 [10].
• Significantly, EGF stimulated autophosphorylation of the EGFR of APC-10 was 8-10-fold lower than that of PC-10 [7].

Analytical, diagnostic and therapeutic context of ANAPC10

• Complementary DNA microarray was performed to compare SW48(CMV-DOC-1) with SW48, which lacks DOC-1 [1].
• RESULTS: Successful transfection of DOC-1 was confirmed by real-time polymerase chain reaction and by Western blot [1].

References