The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

Xic  -  X chromosome inactivation center

Mus musculus

Synonyms: Cg, Xce
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Xce


High impact information on Xce

  • In mammals, dosage compensation is achieved by X inactivation and is regulated in cis by the X-inactivation centre (Xic) and Xist [3].
  • The Xic controls X-chromosome counting, choice of X to inactivate and initiation of silencing [3].
  • Tsix RNA has no conserved ORFs, is seen exclusively in the nucleus and is localized at Xic [3].
  • Xist maps within Xic (refs 7-10) and is necessary in cis for inactivation [4].
  • These results argue that the Xic is contained within 450 kb and that these sequences are sufficient for chromosome counting, choosing, and initiation of X inactivation [5].

Biological context of Xce

  • In mice a further genetic element, the X chromosome-controlling element (Xce), is also known to influence the choice of which of the two X chromosomes is inactivated [6].
  • Analysis of this region in various Xce strains has revealed a correlation between the strength of the Xce allele carried and the methylation status of this region [6].
  • Xce haplotypes show modified methylation in a region of the active X chromosome lying 3' to Xist [6].
  • We did not identify any autosomal loci with significant associations and thus show conclusively that Xce is the only major locus to influence X inactivation patterns in the crosses analyzed [7].
  • Subsequent analysis of recombinant chromosomes allowed for the establishment of a maximum 1.85-Mb candidate region for the Xce locus [7].

Anatomical context of Xce

  • In addition to the relative activity of the PGK-1 allozymes, we also measured the absolute activity of PGK-1 in oocytes obtained from three types of Xce homozygous females.(ABSTRACT TRUNCATED AT 250 WORDS)[8]
  • The work reported here was designed to elucidate whether the nonrandom inactivation of the imprinted XP in yolk-sac endoderm could be modified, or even overridden, by the effect of different Xce alleles [9].
  • Using the modified Kanda method we have therefore studied the proportion of cells at metaphase with the XP inactive in separated yolk-sac endoderm and mesoderm of mouse embryos heterozygous for a marker X chromosome (Cattanach's translocation) carrying different Xce alleles on XP and XM [9].
  • However, the influence of the Xce locus on the randomness of X-inactivation in embryos seems to operate also in ES cell lines [10].
  • Imprinted XCI, normally found in preimplantation embryos and extraembryonic tissues, was not observed in blastocysts or placentae from later stage clones, although fetuses recapitulated the Xce effect [11].

Associations of Xce with chemical compounds

  • Estrogen plays a significant role in regulating the mRNA levels of different Cgs/Sgs suggesting functional and regulatory differences in Cg/Sg proteins [12].
  • Combined in situ hybridization and immunohistochemistry using rat pituitary revealed that the glycoprotein hormone-secreting cells expressed all three Cg/Sg mRNAs in approximately equal amounts [12].
  • CCK(2) receptor antagonists containing the conformationally constrained phenylalanine derivatives, including the new amino acid Xic [13].

Other interactions of Xce

  • The recently characterized Xist sequence (X-inactive-specific transcript) is thought to be a possible candidate for Xic [6].
  • This finding also demonstrates that the timing of reactivation of maternal Pgk-1 is not influenced by the Xce locus [8].
  • Critical control sequences in the Xic include the non-coding RNAs Xist and Tsix, and long-range chromatin elements [14].
  • As Ta25H/+ females show the striped X-inactivation coat pattern, the putative X-inactivation centre, Xce, which lies close to Ta, cannot be located within the region deleted [15].
  • Cloning and characterization of a murine brain specific gene Bpx and its human homologue lying within the Xic candidate region [16].

Analytical, diagnostic and therapeutic context of Xce

  • Using the quantitative RT-PCR single nucleotide primer extension (SNuPE) assay, we found that the amount of Xist RNA in adult kidney of three mouse strains was less than approximately 2000 transcripts per cell, with only modest differences between strains carrying different Xce alleles [10].
  • Transgenesis represents a stringent test for defining the minimal region that can carry out the functions attributed to the Xic [17].
  • Xist, a gene mapping to the X-inactivation centre (Xic), is exclusively expressed from the inactive X-chromosome and is thought to be involved in the initiation of X-inactivation [18].
  • To gain some insight into the regulation and possible functions of specific Cg/Sg members, female rats were ovariectomized for different periods with and without estrogen replacement and the pituitaries were subsequently analyzed by in situ hybridization and Northern hybridization analyses [12].


  1. Genetic analysis of the mouse X inactivation center defines an 80-kb multifunction domain. Lee, J.T., Lu, N., Han, Y. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  2. Lack of evidence that the XqYq pairing tips at meiosis in the mouse show hypersensitivity to DNAse I. Separovic, E.R., Chandley, A.C. Chromosoma (1987) [Pubmed]
  3. Tsix, a gene antisense to Xist at the X-inactivation centre. Lee, J.T., Davidow, L.S., Warshawsky, D. Nat. Genet. (1999) [Pubmed]
  4. Role of the region 3' to Xist exon 6 in the counting process of X-chromosome inactivation. Clerc, P., Avner, P. Nat. Genet. (1998) [Pubmed]
  5. A 450 kb transgene displays properties of the mammalian X-inactivation center. Lee, J.T., Strauss, W.M., Dausman, J.A., Jaenisch, R. Cell (1996) [Pubmed]
  6. Xce haplotypes show modified methylation in a region of the active X chromosome lying 3' to Xist. Courtier, B., Heard, E., Avner, P. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  7. Genetic control of X chromosome inactivation in mice: definition of the Xce candidate interval. Chadwick, L.H., Pertz, L.M., Broman, K.W., Bartolomei, M.S., Willard, H.F. Genetics (2006) [Pubmed]
  8. Expression of X-linked phosphoglycerate kinase in early mouse embryos homozygous at the Xce locus. Krietsch, W.K., Fehlau, M., Renner, P., Bücher, T., Fundele, R. Differentiation (1986) [Pubmed]
  9. Interaction between the Xce locus and imprinting of the paternal X chromosome in mouse yolk-sac endoderm. Rastan, S., Cattanach, B.M. Nature (1983) [Pubmed]
  10. Quantitative RT-PCR assays show Xist RNA levels are low in mouse female adult tissue, embryos and embryoid bodies. Buzin, C.H., Mann, J.R., Singer-Sam, J. Development (1994) [Pubmed]
  11. X chromosome reactivation and regulation in cloned embryos. Nolen, L.D., Gao, S., Han, Z., Mann, M.R., Gie Chung, Y., Otte, A.P., Bartolomei, M.S., Latham, K.E. Dev. Biol. (2005) [Pubmed]
  12. Chromogranin A, chromogranin B and secretogranin II mRNAs in the pituitary and adrenal glands of various mammals. Regulation of chromogranin A, chromogranin B and secretogranin II mRNA levels by estrogen. Lloyd, R.V., Hawkins, K., Jin, L., Kulig, E., Fields, K. Lab. Invest. (1992) [Pubmed]
  13. CCK(2) receptor antagonists containing the conformationally constrained phenylalanine derivatives, including the new amino acid Xic. Gibson, S.E., Guillo, N., Jones, J.O., Buck, I.M., Kalindjian, S.B., Roberts, S., Tozer, M.J. European journal of medicinal chemistry. (2002) [Pubmed]
  14. Transient colocalization of X-inactivation centres accompanies the initiation of X inactivation. Bacher, C.P., Guggiari, M., Brors, B., Augui, S., Clerc, P., Avner, P., Eils, R., Heard, E. Nat. Cell Biol. (2006) [Pubmed]
  15. Genetic and molecular evidence of an X-chromosome deletion spanning the tabby (Ta) and testicular feminization (Tfm) loci in the mouse. Cattanach, B.M., Rasberry, C., Evans, E.P., Dandolo, L., Simmler, M.C., Avner, P. Cytogenet. Cell Genet. (1991) [Pubmed]
  16. Cloning and characterization of a murine brain specific gene Bpx and its human homologue lying within the Xic candidate region. Rougeulle, C., Avner, P. Hum. Mol. Genet. (1996) [Pubmed]
  17. Xist yeast artificial chromosome transgenes function as X-inactivation centers only in multicopy arrays and not as single copies. Heard, E., Mongelard, F., Arnaud, D., Avner, P. Mol. Cell. Biol. (1999) [Pubmed]
  18. XIST expression from the maternal X chromosome in human male preimplantation embryos at the blastocyst stage. Ray, P.F., Winston, R.M., Handyside, A.H. Hum. Mol. Genet. (1997) [Pubmed]
WikiGenes - Universities