Disease relevance of Dnajc5

• The DNA coding for the circumsporozoite protein of Plasmodium falciparum (CSP; aa 1-412) has been placed under the control of the mycobacterial promoter derived from the gene encoding the 64-kDa antigen of Mycobacterium bovis-BCG [1].
• Inoculation of the recombinant BCG to mice did not induce humoral response to CSP nor proliferative response to CSP Th2R CD4+ T lymphocyte epitope [1].
• OBJECTIVE: The recent success of a Plasmodium falciparum malaria vaccine consisting of circumsporozoite (CS) protein (CSP) T and B cell epitopes has rekindled interest in the development of a pre-erythrocytic vaccine [2].
• METHODS: Well-characterized CSP-specific neutralizing B cell epitopes and a 'universal' T cell epitope were combined with a particulate carrier platform, the hepatitis B core antigen (HBcAg), to produce a novel pre-erythrocytic vaccine candidate [2].

High impact information on Dnajc5

• We examined the mechanism of the decrease in CSP after tunicamycin treatment [3].
• Using tunicamycin, we have investigated the role of glycosylation in the biosynthesis, processing and turnover of CSP, the major cell surface glycoprotein of chick embryo fibroblasts (CEF) [3].
• The rate of CSP biosynthesis as measured by pulse-labeling with 14C-leucine was not altered [3].
• Both procollagen and CSP secretion into culture medium were also not increased in AD6, a glycosylation-deficient, mutant mouse 3T3 cell line compared to wild-type cells [3].
• We have isolated such a major cell surface glycoprotein from chick embryo fibroblasts; this protein, CSP, is decreased after transformation [4].

Biological context of Dnajc5

• Cysteine string protein (CSP) is an insulin secretory granule-associated protein regulating beta-cell exocytosis [5].
• The purified material, when injected into mice, generated only modest antibody responses, although antisera from immunized mice reacted with control CSP antigens carrying or not the major immunodominant repeat region [6].

Anatomical context of Dnajc5

• The synaptic vesicle protein CSP alpha prevents presynaptic degeneration [7].
• We conclude that CSP is associated with insulin secretory granules and that CSP participates in the molecular regulation of insulin exocytosis by mechanisms not involving changes in the activity of voltage-gated Ca2+-channels [5].
• Punctate CSP-like immunoreactivity (CSP-LI) was demonstrated in most islets of Langerhans cells, acinar cells and nerve fibers of the rat pancreas [5].
• In addition, the interaction of Csp1 with syntaxin 4 suggests that this Csp isoform may play a role in insulin-stimulated fusion of GSVs with the plasma membrane [8].
• Immunization with either DNA encoding the PyCSP or irradiated sporozoites demonstrated that this CTL epitope is subdominant since it is not recognized in the context of whole CSP immunization [9].

Other interactions of Dnajc5

• The synaptic vesicle protein, cysteine-string protein, is associated with the plasma membrane in 3T3-L1 adipocytes and interacts with syntaxin 4 [8].

Analytical, diagnostic and therapeutic context of Dnajc5

• Tunicamycin had only a slight effect on the initial times and rates of CSP appearance on the cell surface; some apparent intracellular redistribution of CSP was detected by immunofluorescence [3].
• In situ hybridization and RT-PCR analysis demonstrated CSP mRNA expression in insulin-producing cells [5].
• One clone (B6), produced by the fusion of spleen cells from mice that had been given three immunizations, secreted an IgG antibody that did produce a CSP response on the sporozoite and neutralized the infectivity of the parasite [10].

References