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Gene Review

Dnajc5  -  DnaJ (Hsp40) homolog, subfamily C, member 5

Rattus norvegicus

Synonyms: CSP, Csp, Cysteine string protein, DnaJ homolog subfamily C member 5
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Disease relevance of Dnajc5


High impact information on Dnajc5


Biological context of Dnajc5


Anatomical context of Dnajc5


Associations of Dnajc5 with chemical compounds

  • A rat brain complementary DNA (cDNA) encoding CSP was isolated and sequence analysis predicts a protein with 86% identity to Torpedo CSP [10].
  • In conclusion, the close association of cysteine-string protein with synaptic vesicles tethered to synaptic ribbons in inner hair cells and its synchronized expression with the appearance and maturation of the cochlear potentials strongly suggest that this protein plays a fundamental role in sound-evoked glutamate release by inner hair cells [8].
  • In the present study, reverse transcription-polymerase chain reaction analysis confirmed the induction of CSP at mRNA levels in rat frontal cortex after chronic treatment with two different classes of antidepressants, imipramine or sertraline [11].
  • Changes in mRNA levels for heat-shock/stress proteins (Hsp) and a secretory vesicle associated cysteine-string protein (Csp1) after amphetamine (AMPH) exposure [12].
  • Here we have examined the effects of huntingtin fragments (exon 1) with (huntingtin(exon1/exp)) and without (huntingtin(exon1/nonexp)) expanded polyglutamine (polyQ) tracts on the CSP chaperone system [4].

Physical interactions of Dnajc5

  • Binding assays in vitro using recombinant proteins confirmed a direct interaction between the two proteins and demonstrated that the PKA-phosphorylated form of CSP binds synaptotagmin with approximately an order of magnitude lower affinity than the non-phosphorylated form [6].

Other interactions of Dnajc5


Analytical, diagnostic and therapeutic context of Dnajc5

  • Western blotting revealed that the proportion of phosphorylated CSP (P-CSP) varies between distinct brain regions and also exhibits developmental regulation, with P-CSP highest early in development [5].
  • Here we have examined the self-association of CSP to form oligomers that are stable upon SDS-PAGE [9].
  • RT-PCR products for Csp1 were produced in total RNA obtained from brain but not from cultured astrocytes, suggesting that the Csp1 mRNA measured by RT-PCR is of neuronal origin [12].
  • To study this issue, we analyzed the effects of chronic lithium administration (21 days) on csp mRNA levels in rat brain using in situ hybridization [16].
  • In this context, we observed recently that lithium at therapeutically relevant concentrations enhanced expression of cysteine string protein (csp) at the level of both mRNA and protein, in cell culture and in rat brain [16].


  1. Confluent BALB/c 3T3 cells monolayer provides selective and efficient growth of neoplastic cells. Kido, Y., Mitsudomi, T., Kuwano, H., Yano, K., Matsuoka, H., Sugimachi, K. Cancer Lett. (1990) [Pubmed]
  2. Cysteine string protein immunoreactivity in the nervous system and adrenal gland of rat. Kohan, S.A., Pescatori, M., Brecha, N.C., Mastrogiacomo, A., Umbach, J.A., Gundersen, C.B. J. Neurosci. (1995) [Pubmed]
  3. Characterization of the G alpha(s) regulator cysteine string protein. Natochin, M., Campbell, T.N., Barren, B., Miller, L.C., Hameed, S., Artemyev, N.O., Braun, J.E. J. Biol. Chem. (2005) [Pubmed]
  4. Cysteine string protein (CSP) inhibition of N-type calcium channels is blocked by mutant huntingtin. Miller, L.C., Swayne, L.A., Chen, L., Feng, Z.P., Wacker, J.L., Muchowski, P.J., Zamponi, G.W., Braun, J.E. J. Biol. Chem. (2003) [Pubmed]
  5. Phosphorylation of cysteine string protein in the brain: developmental, regional and synaptic specificity. Evans, G.J., Morgan, A. Eur. J. Neurosci. (2005) [Pubmed]
  6. Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I. Evans, G.J., Morgan, A. Biochem. J. (2002) [Pubmed]
  7. Interaction of cysteine string proteins with the alpha1A subunit of the P/Q-type calcium channel. Leveque, C., Pupier, S., Marqueze, B., Geslin, L., Kataoka, M., Takahashi, M., De Waard, M., Seagar, M. J. Biol. Chem. (1998) [Pubmed]
  8. Cysteine-string protein in inner hair cells of the organ of Corti: synaptic expression and upregulation at the onset of hearing. Eybalin, M., Renard, N., Aure, F., Safieddine, S. Eur. J. Neurosci. (2002) [Pubmed]
  9. Oligomerization characteristics of cysteine string protein. Swayne, L.A., Blattler, C., Kay, J.G., Braun, J.E. Biochem. Biophys. Res. Commun. (2003) [Pubmed]
  10. Cysteine string protein, a DnaJ family member, is present on diverse secretory vesicles. Braun, J.E., Scheller, R.H. Neuropharmacology (1995) [Pubmed]
  11. Induction of cysteine string protein after chronic antidepressant treatment in rat frontal cortex. Yamada, M., Yamada, M., Yamazaki, S., Takahashi, K., Nara, K., Ozawa, H., Yamada, S., Kiuchi, Y., Oguchi, K., Kamijima, K., Higuchi, T., Momose, K. Neurosci. Lett. (2001) [Pubmed]
  12. Changes in mRNA levels for heat-shock/stress proteins (Hsp) and a secretory vesicle associated cysteine-string protein (Csp1) after amphetamine (AMPH) exposure. Bowyer, J.F., Davies, D.L. Ann. N. Y. Acad. Sci. (1999) [Pubmed]
  13. Interaction of SNARE complexes with P/Q-type calcium channels in rat cerebellar synaptosomes. Martin-Moutot, N., Charvin, N., Leveque, C., Sato, K., Nishiki, T., Kozaki, S., Takahashi, M., Seagar, M. J. Biol. Chem. (1996) [Pubmed]
  14. Molecular determinants of cysteine string protein modulation of N-type calcium channels. Miller, L.C., Swayne, L.A., Kay, J.G., Feng, Z.P., Jarvis, S.E., Zamponi, G.W., Braun, J.E. J. Cell. Sci. (2003) [Pubmed]
  15. Munc-18-1 and cysteine string protein (csp) in pinealocytes of the gerbil pineal gland. Redecker, P., Pabst, H., Grube, D. Cell Tissue Res. (1998) [Pubmed]
  16. Dietary lithium induces regional increases of mRNA encoding cysteine string protein in rat brain. Cordeiro, M.L., Gundersen, C.B., Umbach, J.A. J. Neurosci. Res. (2003) [Pubmed]
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