Disease relevance of DNAJC5

• These results help to clarify previous experiments in which CSP, a purified LETS protein, partially restored a fibroblastic phenotype to cells transformed by tumor viruses [1].
• Several studies, including AIDS Clinical Trials Group (ACTG) 116B/117, the Veterans Administration Study VA CSP 298 and, more recently, ACTG 175, have indicated that viral load monitoring is a powerful predictor of disease progression and clinical outcome [2].
• Interstitial pneumonitis was more frequent among patients given ATG/CSP/MP [3].
• METHODS: In this prospective, controlled study, 34 of the 72 consecutive patients with symptomatic uterovaginal prolapse were treated with colposacropexy with uterus conservation (hysterocolposacropexy, HSP) and the other 38 with hysterectomy followed by sacropexy (CSP) [4].
• Recurrent low-grade cystoceles developed in 1/38 (2.6%) in the CSP group and in 5/34 (14.7%) in the HSP group (p=NS), recurrent low-grade rectocele developed in 6/38 (15.8%) and in 3/34 (8.8%) patients respectively (p=NS) [4].

Psychiatry related information on DNAJC5

• HIV+ subjects with CSP were more likely to complain of sensory changes and performed more poorly on specific neuropsychological tests when compared with HIV+ subjects without CSP [5].

High impact information on DNAJC5

• When a transformed cell line with poor adhesiveness (MC5-5) was treated with CSP (a major surface glycoprotein of normal cells) which increases adhesiveness to substratum, the cells formed extensive microfilament bundles without any decrease in growth [6].
• Either antibody reacts with the synthetic peptide, cross-links the CS protein on the membrane of the parasite giving the CSP reaction, and neutralizes the infectivity of sporozoites [7].
• These specialized co-chaperones include auxilin, cysteine string protein, Tom70, UNC-45 and homologs of Bag-1 [8].
• The major cell surface glycoprotein of chick embryo fibroblasts, cellular fibronectin (formerly known as CSP or LETS protein), was purified and used to produce monospecific antisera [9].
• Our results are consistent with a role for CSP in cell adhesion and morphology but not in growth control [10].

Biological context of DNAJC5

• Mutational analysis of cysteine-string protein function in insulin exocytosis [11].
• The synaptic vesicle-associated cysteine-string protein (CSP) is important for synaptic transmission [12].
• PKA phosphorylation of CSP reduced its binding to syntaxin by 10-fold but had little effect on its interaction with HSC70 or G-protein subunits [13].
• Cysteine string protein monitors late steps in cystic fibrosis transmembrane conductance regulator biogenesis [14].
• Csp knockdown by RNA interference produced a 5-fold increase in mature CFTR and augmented cAMP-stimulated CFTR currents [14].

Anatomical context of DNAJC5

• Furthermore, studies here suggest that both GAD(65) and GAD(67) are associated with synaptic vesicles along with HSC70 and CSP [15].
• Cysteine string protein (CSP), a secretory vesicle membrane protein and a member of the dnaJ family of co-chaperones, may assist in performing this function [16].
• In contrast, endogenous CSP was phosphorylated in synaptosomes, PC12 cells, and chromaffin cells [13].
• Cysteine-string protein isoform beta (Cspbeta) is targeted to the trans-Golgi network as a non-palmitoylated CSP in clonal beta-cells [17].
• On qualitative analysis, we found a high incidence of midline developmental abnormalities (cavum septum pellucidum, or CSP) [18].

Associations of DNAJC5 with chemical compounds

• Our results show that the guanine nucleotide exchange activity of full-length CSP is, in turn, regulated by Hsc70-SGT [19].
• In addition, we provide an update on the mammalian isoforms of cysteine string protein following the recent discovery of two novel cysteine string proteins [16].
• It is also notable that only 2 (5%) of 39 patients who received the combination of methotrexate and cyclosporine (MTX/CSP) for the prevention of GVHD developed grade II acute GVHD, and none developed grades III to IV acute GVHD [20].
• In vitro 32P-phosphorylation studies revealed that Akt phosphorylates CSP on serine 10 [21].
• Antipeptide antibodies against a Torpedo cysteine-string protein [22].

Physical interactions of DNAJC5

• Cysteine string protein interacts with and modulates the maturation of the cystic fibrosis transmembrane conductance regulator [23].

Other interactions of DNAJC5

• Fourth, HSC70 and CSP were co-purified with GAD by specific anti-GAD immunoaffinity columns [15].
• Nevertheless, Csp H43Q still increased immature CFTR [14].
• Like Hdj-2, Csp reduced the aggregation of NBD1 in vitro in the absence of Hsp70 [14].
• Protein kinase B/Akt is a novel cysteine string protein kinase that regulates exocytosis release kinetics and quantal size [21].
• Regulation of airway mucin secretion by MARCKS protein involves the chaperones heat shock protein 70 and cysteine string protein [24].

Analytical, diagnostic and therapeutic context of DNAJC5

• Both cysteine string protein (CSP) and soluble NSF attachment protein-alpha (alpha-SNAP) were phosphorylated by PKA in vitro, but immunoprecipitation of cellular alpha-SNAP failed to detect (32)P incorporation [13].
• CSP #221 is a randomized multiinstitutional clinical trial to assess the efficacy of 10 d of perioperative total parenteral nutrition (TPN) in reducing morbidity and mortality in malnourished patients undergoing intraperitoneal and/or intrathoracic operations [25].
• Given that further progress in instrumental design and engineering of dedicated, highly enantioselective CSPAs can be achieved, CPC may offer a viable alternative to CSP-based HPLC for preparative-scale enantiomer separation [26].
• However, both a yeast-derived Ty virus-like particle expressing the optimal nine-amino acid epitope SYIPSAEKI from the CS protein (CSP-VLP) and a lipid-tailed peptide of this same sequence induced high levels of the major histocompatibility complex (MHC) class I-restricted CTL with one and three subcutaneous immunizations, respectively [27].
• Electrical stimulation of both the dorsum and the palm evoked a CSP (latency 65 +/- 5 ms), although the reflex threshold was significantly lower after stimulation of the palm [28].

References