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Gene Review

Eef1a1  -  eukaryotic translation elongation factor 1...

Mus musculus

Synonyms: EF-1-alpha-1, EF-Tu, Eef1a, Elongation factor 1-alpha 1, Elongation factor Tu, ...
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Disease relevance of Eef1a1


High impact information on Eef1a1


Biological context of Eef1a1


Anatomical context of Eef1a1

  • In adult mammalian tissues, isoform eEF1A-1 is present in all tissues except neurons, cardiomyocytes, and myotubes, where its isoform, eEF1A-2, is the only form expressed [7].
  • Here we show that Prdx-I coimmunoprecipitates with eEF1A-2 from extracts of both cultured cells and mouse tissues expressing this protein, but it does not do so with its isoform, eEF1A-1, even though the latter is abundantly present [7].
  • Both forms of eEF1A have been characterized to function in the protein elongation step of translation, and eEF1A-1 is shown to possess additional non-canonical roles in actin binding/bundling, microtubule bundling/severing, and cellular transformation processes [7].
  • Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development [8].
  • Peptide elongation factor eEF1A-2/S1, which shares 92% homology with eEF1A-1/EF-1alpha, is exclusively expressed in brain, heart, and skeletal muscle [4].

Associations of Eef1a1 with chemical compounds

  • Several GTP binding proteins, including EF-Tu, Ypt1, rab-5, and FtsY, and adenylosuccinate synthetase have been reported to bind xanthine nucleotides when the conserved aspartate residue in the NKXD motif was changed to asparagine [9].
  • Two-dimensional gel electrophoretic (NEPHGE) analysis of proteins from mouse 3T3B and 3T3B/SV40 cells labelled with [methyl-3H]methionine in the presence of cycloheximide have revealed that the elongation factor 1 alpha (EF-1 alpha) in these cells is methylated and that the extent of methylation is higher in the SV40 transformed cell type [10].
  • Ethanolamine containing modifications of cytosolic proteins have only been described for elongation factor 1 alpha (EF-1 alpha) which contains two ethanolamine-phosphoglycerol moieties at Glu 301 and 374 (Whiteheart et al. (1989) J. Biol. Chem. 264, 14334-14341 and Dever et al. (1989) J. Biol. Chem. 264, 20518-20525) [11].

Other interactions of Eef1a1

  • The observed gradual decrease in FasL mRNA could not be attributed to a generalized degradation of cellular RNA during atresia, as evidenced by the presence of constitutive expression of elongation factor 1 alpha (EF-1 alpha) mRNA in murine ovaries and granulosa cells treated with PMSG [12].
  • The observed decreases in mRNA of Fas could not be attributed to a generalized degradation of cellular RNA during atresia, as evidenced by the presence of intact 18S and 28S ribosomal RNA as well as constitutive expression of EF-1 alpha mRNA in atretic follicles [13].
  • Furthermore, the alpha3/beta5 region corresponds to an exchange factor contact site in both EF-Tu and Ras, suggesting that the mechanisms by which seven-transmembrane receptors and exchange factors catalyze nucleotide exchange may share common elements [14].
  • Freshly isolated and thawed hepatocytes were transduced in suspension with lentiviral vectors expressing the GFP gene under the control of a ubiquitous promoter (EF1-alpha) and the apolipoprotein A-II promoter [15].
  • The mRNA for poly(A)-binding protein behaved similarly to EF-1 alpha [16].

Analytical, diagnostic and therapeutic context of Eef1a1


  1. Expression of a gene for mouse eucaryotic elongation factor Tu during murine erythroleukemic cell differentiation. Roth, W.W., Bragg, P.W., Corrias, M.V., Reddy, N.S., Dholakia, J.N., Wahba, A.J. Mol. Cell. Biol. (1987) [Pubmed]
  2. Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae. Nagata, S., Nagashima, K., Tsunetsugu-Yokota, Y., Fujimura, K., Miyazaki, M., Kaziro, Y. EMBO J. (1984) [Pubmed]
  3. The lethal mutation of the mouse wasted (wst) is a deletion that abolishes expression of a tissue-specific isoform of translation elongation factor 1alpha, encoded by the Eef1a2 gene. Chambers, D.M., Peters, J., Abbott, C.M. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  4. Peptide elongation factor eEF1A-2/S1 expression in cultured differentiated myotubes and its protective effect against caspase-3-mediated apoptosis. Ruest, L.B., Marcotte, R., Wang, E. J. Biol. Chem. (2002) [Pubmed]
  5. Tissue-specific expression in mammalian brain, heart, and muscle of S1, a member of the elongation factor-1 alpha gene family. Lee, S., Francoeur, A.M., Liu, S., Wang, E. J. Biol. Chem. (1992) [Pubmed]
  6. Isolation and characterization of the rat chromosomal gene for a polypeptide (pS1) antigenically related to statin. Ann, D.K., Moutsatsos, I.K., Nakamura, T., Lin, H.H., Mao, P.L., Lee, M.J., Chin, S., Liem, R.K., Wang, E. J. Biol. Chem. (1991) [Pubmed]
  7. Mouse translation elongation factor eEF1A-2 interacts with Prdx-I to protect cells against apoptotic death induced by oxidative stress. Chang, R., Wang, E. J. Cell. Biochem. (2007) [Pubmed]
  8. Immuno-characterization of the switch of peptide elongation factors eEF1A-1/EF-1alpha and eEF1A-2/S1 in the central nervous system during mouse development. Pan, J., Ruest, L.B., Xu, S., Wang, E. Brain Res. Dev. Brain Res. (2004) [Pubmed]
  9. Characterization of a Goalpha mutant that binds xanthine nucleotides. Yu, B., Slepak, V.Z., Simon, M.I. J. Biol. Chem. (1997) [Pubmed]
  10. Methylation of elongation factor 1 alpha in mouse 3T3B and 3T3B/SV40 cells. Coppard, N.J., Clark, B.F., Cramer, F. FEBS Lett. (1983) [Pubmed]
  11. Incorporation of [3H]ethanolamine into a single cytosolic protein in a cell free system: ethanolaminylation of EF-1 alpha in vitro. Whiteheart, S.W., Hart, G.W. Arch. Biochem. Biophys. (1994) [Pubmed]
  12. Expression of Fas ligand in murine ovary. Guo, M.W., Xu, J.P., Mori, E., Sato, E., Saito, S., Mori, T. Am. J. Reprod. Immunol. (1997) [Pubmed]
  13. Identification of Fas antigen associated with apoptotic cell death in murine ovary. Guo, M.W., Mori, E., Xu, J.P., Mori, T. Biochem. Biophys. Res. Commun. (1994) [Pubmed]
  14. A surface-exposed region of G(salpha) in which substitutions decrease receptor-mediated activation and increase receptor affinity. Grishina, G., Berlot, C.H. Mol. Pharmacol. (2000) [Pubmed]
  15. Efficient ex vivo gene transfer into non-human primate hepatocytes using HIV-1 derived lentiviral vectors. Parouchev, A., Nguyen, T.H., Dagher, I., Mainot, S., Groyer-Picard, M.T., Branger, J., Gonin, P., Di Santo, J., Franco, D., Gras, G., Weber, A. J. Hepatol. (2006) [Pubmed]
  16. Translational repression of EF-1 alpha mRNA in vitro. Slobin, L.I., Rao, M.N. Eur. J. Biochem. (1993) [Pubmed]
  17. A common multiple cloning site in a set of vectors for expression of eukaryotic genes in mammalian, insect and bacterial cells. Pallisgaard, N., Pedersen, F.S., Birkelund, S., Jørgensen, P. Gene (1994) [Pubmed]
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