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Gene Review

BPyV_gp1  -  agnoprotein

Bovine polyomavirus

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Disease relevance of BPyVgp1


High impact information on BPyVgp1


Biological context of BPyVgp1

  • Previous work showed that in CV1 cells infected with dl1066 and dl1140, levels of viral DNA replication and of late capsid protein accumulation were only slightly reduced and the failure to accumulate agnoprotein was not likely to be the major factor responsible for the mutants' growth defect [7].
  • Of six independently isolated pseudorevertants, all had a missense mutation in a serine codon, near the beginning of the agnogene, that would cause replacement of serine at position 7, 11, or 17 in the agnoprotein by a hydrophobic amino acid [8].
  • The agnoprotein reading frame, depending on how the mRNA precursor is spliced, is either not contained in the mRNA or is terminated near the VP2 AUG. Under these conditions, the ability of the agnoprotein AUG to block downstream CAT AUG utilization was found to be minimal in our assay [9].
  • In this study, we investigated whether DNA elements located downstream of the JCV late promoter, encompassing the late leader peptide region, which encodes agnoprotein, play regulatory roles in the JCV lytic cycle [10].
  • Here we demonstrate that in the absence of other viral proteins, expression of Agnoprotein can inhibit cell growth by deregulating cell progression through the cell cycle stages [11].

Anatomical context of BPyVgp1


Associations of BPyVgp1 with chemical compounds

  • Here we report that agnoprotein expression sensitized cells to the cytotoxic effects of the DNA-damaging agent cisplatin [16].
  • Moreover, conservative substitutions at PKC phosphorylation sites (Ser7, Ser11, and Thr21 to Asp) resulted in a viable virus, which further demonstrate the importance of these sites on agnoprotein function [17].
  • Agnoprotein contains several potential phosphorylation sites, including Ser7, Ser11, and Thr21, which are potential targets for the serine/threonine-specific protein kinase C (PKC) [17].
  • A leucine zipper-like motif present in agnoprotein 1a is considered to be involved in DNA binding [18].

Physical interactions of BPyVgp1

  • Our results suggest that the agnoprotein interacts in a specific way with VP1 during the late stages of viral development [8].

Analytical, diagnostic and therapeutic context of BPyVgp1


  1. Simian virus 40 agnoprotein facilitates perinuclear-nuclear localization of VP1, the major capsid protein. Carswell, S., Alwine, J.C. J. Virol. (1986) [Pubmed]
  2. Identification of three new JC virus proteins generated by alternative splicing of the early viral mRNA. Trowbridge, P.W., Frisque, R.J. J. Neurovirol. (1995) [Pubmed]
  3. Simian virus 40 host range/helper function mutations cause multiple defects in viral late gene expression. Stacy, T., Chamberlain, M., Cole, C.N. J. Virol. (1989) [Pubmed]
  4. Expression of human neurotropic polyomavirus JCV late gene product agnoprotein in human medulloblastoma. Del Valle, L., Gordon, J., Enam, S., Delbue, S., Croul, S., Abraham, S., Radhakrishnan, S., Assimakopoulou, M., Katsetos, C.D., Khalili, K. J. Natl. Cancer Inst. (2002) [Pubmed]
  5. Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation. Suzuki, T., Okada, Y., Semba, S., Orba, Y., Yamanouchi, S., Endo, S., Tanaka, S., Fujita, T., Kuroda, S., Nagashima, K., Sawa, H. J. Biol. Chem. (2005) [Pubmed]
  6. Attenuation of late simian virus 40 mRNA synthesis is enhanced by the agnoprotein and is temporally regulated in isolated nuclear systems. Hay, N., Aloni, Y. Mol. Cell. Biol. (1985) [Pubmed]
  7. Simian virus 40 large T antigen host range domain functions in virion assembly. Spence, S.L., Pipas, J.M. J. Virol. (1994) [Pubmed]
  8. Suppression of a VP1 mutant of simian virus 40 by missense mutations in serine codons of the viral agnogene. Margolskee, R.F., Nathans, D. J. Virol. (1983) [Pubmed]
  9. Translational control of synthesis of simian virus 40 late proteins from polycistronic 19S late mRNA. Dabrowski, C., Alwine, J.C. J. Virol. (1988) [Pubmed]
  10. Human polyomavirus JCV late leader peptide region contains important regulatory elements. Akan, I., Sariyer, I.K., Biffi, R., Palermo, V., Woolridge, S., White, M.K., Amini, S., Khalili, K., Safak, M. Virology (2006) [Pubmed]
  11. Evidence for dysregulation of cell cycle by human polyomavirus, JCV, late auxiliary protein. Darbinyan, A., Darbinian, N., Safak, M., Radhakrishnan, S., Giordano, A., Khalili, K. Oncogene (2002) [Pubmed]
  12. Expression of JC virus agnoprotein in progressive multifocal leukoencephalopathy brain. Okada, Y., Sawa, H., Endo, S., Orba, Y., Umemura, T., Nishihara, H., Stan, A.C., Tanaka, S., Takahashi, H., Nagashima, K. Acta Neuropathol. (2002) [Pubmed]
  13. Dissociation of heterochromatin protein 1 from lamin B receptor induced by human polyomavirus agnoprotein: role in nuclear egress of viral particles. Okada, Y., Suzuki, T., Sunden, Y., Orba, Y., Kose, S., Imamoto, N., Takahashi, H., Tanaka, S., Hall, W.W., Nagashima, K., Sawa, H. EMBO Rep. (2005) [Pubmed]
  14. Expression of the Rous sarcoma virus env gene from a simian virus 40 late-region replacement vector: effects of upstream initiation codons. Perez, L., Wills, J.W., Hunter, E. J. Virol. (1987) [Pubmed]
  15. Subcellular localization of the simian virus 40 agnoprotein. Nomura, S., Khoury, G., Jay, G. J. Virol. (1983) [Pubmed]
  16. Role of JC virus agnoprotein in DNA repair. Darbinyan, A., Siddiqui, K.M., Slonina, D., Darbinian, N., Amini, S., White, M.K., Khalili, K. J. Virol. (2004) [Pubmed]
  17. Phosphorylation mutants of JC virus agnoprotein are unable to sustain the viral infection cycle. Sariyer, I.K., Akan, I., Palermo, V., Gordon, J., Khalili, K., Safak, M. J. Virol. (2006) [Pubmed]
  18. Avian polyomavirus agnoprotein 1a is incorporated into the virus particle as a fourth structural protein, VP4. Johne, R., Müller, H. J. Gen. Virol. (2001) [Pubmed]
  19. Primary central nervous system lymphoma expressing the human neurotropic polyomavirus, JC virus, genome. Del Valle, L., Enam, S., Lara, C., Miklossy, J., Khalili, K., Gordon, J. J. Virol. (2004) [Pubmed]
  20. Construction and characterization of CV-1P cell lines which constitutively express the simian virus 40 agnoprotein: alteration of plaquing phenotype of viral agnogene mutants. Carswell, S., Resnick, J., Alwine, J.C. J. Virol. (1986) [Pubmed]
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