The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

crn-1  -  Protein CRN-1

Caenorhabditis elegans

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of endonuclease


High impact information on endonuclease


Biological context of endonuclease

  • DNA prepared from four isolates of Trichinella was compared by genomic DNA cross-hybridisation, by electrophoresis following restriction endonuclease digestion and by hybridisation studies using a cloned repetitive DNA sequence from T. spiralis [9].
  • When genomic DNA from the free-living nematode Panagrellus silusiae is digested with the restriction endonuclease BamHI and separated by electrophoresis, a band in the 700 base pair size range is evident after ethidium bromide staining [1].
  • To confirm that transfection was not activating another endonuclease, cells were incubated with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-(O-methyl)-fluoromethylketone; this failed to inhibit chromatin condensation induced by DNase II [6].
  • It encodes a 1,024-amino-acid open reading frame (ORF) containing both apurinic-apyrimidic endonuclease and reverse-transcriptase domains [10].
  • The 3 oldest lineages show target site specificity for unique locations in the genome and encode an endonuclease with an active site similar to certain restriction enzymes [11].

Anatomical context of endonuclease


Associations of endonuclease with chemical compounds

  • Embryonic extracts derived from the nematode Caenorhabditis elegans remove uracil from DNA by the sequential action of uracil-DNA glycosylase and AP (apurinic/apyrimidinic) endonuclease [13].

Other interactions of endonuclease


Analytical, diagnostic and therapeutic context of endonuclease


  1. Genomic arrangement of repeated PS700 elements in the nematode Panagrellus silusiae. Retterath, M.A., Pasternak, J.J. Genome (1990) [Pubmed]
  2. Presence of Mycobacterium avium subspecies paratuberculosis in suspensions of ovine trichostrongylid larvae produced in faecal cultures artificially contaminated with the bacterium. Lloyd, J.B., Whittington, R.J., Fitzgibbon, C., Dobson, R. Vet. Rec. (2001) [Pubmed]
  3. Identification of two apurinic/apyrimidinic endonucleases from Caenorhabditis elegans by cross-species complementation. Shatilla, A., Leduc, A., Yang, X., Ramotar, D. DNA Repair (Amst.) (2005) [Pubmed]
  4. Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis. Hosfield, D.J., Guan, Y., Haas, B.J., Cunningham, R.P., Tainer, J.A. Cell (1999) [Pubmed]
  5. CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation. Parrish, J.Z., Yang, C., Shen, B., Xue, D. EMBO J. (2003) [Pubmed]
  6. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. Krieser, R.J., Eastman, A. J. Biol. Chem. (1998) [Pubmed]
  7. Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells. Smih, F., Rouet, P., Romanienko, P.J., Jasin, M. Nucleic Acids Res. (1995) [Pubmed]
  8. Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging. Simpson, V.J., Johnson, T.E., Hammen, R.F. Nucleic Acids Res. (1986) [Pubmed]
  9. Repetitive DNA as a tool for the identification and comparison of nematode variants: application to Trichinella isolates. Chambers, A.E., Almond, N.M., Knight, M., Simpson, A.J., Parkhouse, R.M. Mol. Biochem. Parasitol. (1986) [Pubmed]
  10. The RTE class of non-LTR retrotransposons is widely distributed in animals and is the origin of many SINEs. Malik, H.S., Eickbush, T.H. Mol. Biol. Evol. (1998) [Pubmed]
  11. NeSL-1, an ancient lineage of site-specific non-LTR retrotransposons from Caenorhabditis elegans. Malik, H.S., Eickbush, T.H. Genetics (2000) [Pubmed]
  12. Characterization of endonuclease activity from excretory/secretory products of a parasitic nematode, Trichinella spiralis. Mak, C.H., Ko, R.C. Eur. J. Biochem. (1999) [Pubmed]
  13. Embryonic extracts derived from the nematode Caenorhabditis elegans remove uracil from DNA by the sequential action of uracil-DNA glycosylase and AP (apurinic/apyrimidinic) endonuclease. Shatilla, A., Ramotar, D. Biochem. J. (2002) [Pubmed]
  14. DLAD, a novel mammalian divalent cation-independent endonuclease with homology to DNase II. Shiokawa, D., Tanuma, S. Nucleic Acids Res. (1999) [Pubmed]
  15. Detection and differentiation of filarial parasites by universal primers and polymerase chain reaction-restriction fragment length polymorphism analysis. Nuchprayoon, S., Junpee, A., Poovorawan, Y., Scott, A.L. Am. J. Trop. Med. Hyg. (2005) [Pubmed]
  16. Major sperm protein genes from Onchocerca volvulus. Scott, A.L., Dinman, J., Sussman, D.J., Yenbutr, P., Ward, S. Mol. Biochem. Parasitol. (1989) [Pubmed]
WikiGenes - Universities