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Gene Review

gst-6  -  Protein GST-6

Caenorhabditis elegans

 
 
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Disease relevance of gst-6

  • Structure of the major cytosolic glutathione S-transferase from the parasitic nematode Onchocerca volvulus [1].
  • Glutathione S-transferase (GST) fusion proteins of individual subclones were produced in Escherichia coli, purified by affinity chromatography and evaluated in ELISA using sera from dogs with infections of E. granulosus, Taenia spp. or nematodes, and helminth-free dog sera [2].
 

High impact information on gst-6

  • Furthermore, phosphopeptide-binding studies revealed that the GST-Crk SH2 domain binds to a tyrosine-phosphorylated peptide corresponding to amino acids 770-781 of Cbl with high affinity [3].
  • A stress-responsive glutathione S-transferase confers resistance to oxidative stress in Caenorhabditis elegans [4].
  • The structural gene for glutathione S-transferase (CeGST1-1) in the fungus Cunninghamella elegans was cloned by screening a cDNA library using a degenerate oligonucleotide probe based on the N-terminal sequence of the purified protein [5].
  • A recombinant glutathione S-transferase (GST) (EC 2.5.1.18) from the parasitic nematode Ascaris suum (AsGST1) displays specific activity with a variety of model substrates and secondary products of lipid peroxidation [6].
  • The subsequently isolated full-length cDNA harbors a 753-bp open reading frame encoding a GST with 268 amino acid residues and a predicted molecular mass of 31 kDa [7].
 

Biological context of gst-6

 

Associations of gst-6 with chemical compounds

 

Analytical, diagnostic and therapeutic context of gst-6

  • Molecular cloning, expression and characterization of a novel class glutathione S-transferase from the fungus Cunninghamella elegans [5].
  • The GST fusion protein 10P1 showed a specificity of 100% in ELISA for diagnosis of E. granulosus infection in dogs despite its relatively low sensitivity [2].
  • Comparison by Western blot analysis implied that this fungal GST had no relationship with mammalian alpha-, mu-, and pi-class GSTs, although it showed a small degree of cross-reactivity with a theta-class GST [10].
  • Four subunits of the cytosolic glutathione S-transferase (GST) in Orthosia gothica fed on willow leaves and a semisynthetic bean diet were purified as separate peaks (subunits 1-4) by a two-step gradient elution from a reverse-phase HPLC column after an initial purification by glutathione-Sepharose 1-chloro-2,4-dinitro-benzene (CDNB) [12].

References

  1. Structure of the major cytosolic glutathione S-transferase from the parasitic nematode Onchocerca volvulus. Perbandt, M., Höppner, J., Betzel, C., Walter, R.D., Liebau, E. J. Biol. Chem. (2005) [Pubmed]
  2. A recombinant antigen with potential for serodiagnosis of Echinococcus granulosus infection in dogs. Gasser, R.B., Lightowlers, M.W., Rickard, M.D. Int. J. Parasitol. (1990) [Pubmed]
  3. Tyrosine-phosphorylated Cbl binds to Crk after T cell activation. Sawasdikosol, S., Chang, J.H., Pratt, J.C., Wolf, G., Shoelson, S.E., Burakoff, S.J. J. Immunol. (1996) [Pubmed]
  4. A stress-responsive glutathione S-transferase confers resistance to oxidative stress in Caenorhabditis elegans. Leiers, B., Kampkötter, A., Grevelding, C.G., Link, C.D., Johnson, T.E., Henkle-Dührsen, K. Free Radic. Biol. Med. (2003) [Pubmed]
  5. Molecular cloning, expression and characterization of a novel class glutathione S-transferase from the fungus Cunninghamella elegans. Cha, C.J., Kim, S.J., Kim, Y.H., Stingley, R., Cerniglia, C.E. Biochem. J. (2002) [Pubmed]
  6. Structural and functional analysis of a glutathione S-transferase from Ascaris suum. Liebau, E., Eckelt, V.H., Wildenburg, G., Teesdale-Spittle, P., Brophy, P.M., Walter, R.D., Henkle-Dührsen, K. Biochem. J. (1997) [Pubmed]
  7. Identification of a stress-responsive Onchocerca volvulus glutathione S-transferase (Ov-GST-3) by RT-PCR differential display. Liebau, E., Eschbach, M.L., Tawe, W., Sommer, A., Fischer, P., Walter, R.D., Henkle-Dührsen, K. Mol. Biochem. Parasitol. (2000) [Pubmed]
  8. Molecular phylogeny of glutathione-S-transferases. Snyder, M.J., Maddison, D.R. DNA Cell Biol. (1997) [Pubmed]
  9. Caenorhabditis elegans as an environmental monitor using DNA microarray analysis. Custodia, N., Won, S.J., Novillo, A., Wieland, M., Li, C., Callard, I.P. Ann. N. Y. Acad. Sci. (2001) [Pubmed]
  10. Purification and characterization of a glutathione S-transferase from the fungus Cunninghamella elegans. Cha, C.J., Coles, B.F., Cerniglia, C.E. FEMS Microbiol. Lett. (2001) [Pubmed]
  11. Proteomic identification of glutathione S-transferases from the model nematode Caenorhabditis elegans. van Rossum, A.J., Brophy, P.M., Tait, A., Barrett, J., Jefferies, J.R. Proteomics (2001) [Pubmed]
  12. The separation and identification of glutathione S-transferase subunits from Orthosia gothica. Egaas, E., Sandvik, M., Svendsen, N.O., Skaare, J.U. Insect Biochem. Mol. Biol. (1995) [Pubmed]
 
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