Disease relevance of Serpinb6a

• Brain ischemia induced by bilateral ligation of the common carotid artery led to an increase in PI-6 mRNA expression in the whole brain, accompanied by the degeneration of hippocampal pyramidal cells [1].
• In the present study, we investigated the developmental expression of PI-6 in the mouse brain and the effect of experimental ischemia on the expression of PI-6 in the adult brain [1].

High impact information on Serpinb6a

• This abnormality was corrected by infusion of purified HCII but not ovalbumin [2].
• Targeted disruption of SPI3/Serpinb6 does not result in developmental or growth defects, leukocyte dysfunction, or susceptibility to stroke [3].
• The SPI6 gene was mapped to the same region on mouse chromosome 13 as Spi3, which encodes the murine homolog of PI-6 [4].
• Our identification of multiple sequence homologs of PI-8 and PI-9, and six new ovalbumin serpins, is consonant with the idea that the larger set of granule and other proteinases known to exist in the mouse (compared with human) is balanced by a larger array of serpins [4].
• Human proteinase inhibitor 6 (PI-6) is a recently described protein belonging to the serine proteinase inhibitor (serpin) superfamily [5].

Biological context of Serpinb6a

• These observations raise the question of the possible role(s) of the Serpinb6 family members in gonad development, gametogenesis, and/or fertilization [6].
• In mice, Spi3/Serpinb6 has a broad tissue distribution [6].
• To determine the temporal and spatial distribution of PI-6, mice were generated in which exon 2 of the PI-6 ortholog SPI3 (Serpinb6) was replaced with a green fluorescent protein (GFP) reporter gene [7].

Anatomical context of Serpinb6a

• Expression of Serpinb6 serpins in germ and somatic cells of mouse gonads [6].
• In the brain stem, two specific motor nuclei, the facial nucleus and the motor nucleus of trigeminal nerve, which are important to active feeding, were strongly positive for PI-6 mRNA [1].
• Second, Serpinb6 mRNA and the protein were strongly expressed in most somatic and visceral motoneurons among cranial nerve nuclei [8].
• First, the forebrain limbic area that expressed neuropsin mRNA contained Serpinb6 mRNA at moderate levels but not the lateral septum [8].

Regulatory relationships of Serpinb6a

• Together, it was suggested that the expression of neuropsin in the brain is regulated entirely by Serpinb6 [8].

Analytical, diagnostic and therapeutic context of Serpinb6a

• Using reverse transcriptase-polymerase chain reaction assays on RNA isolated from 15-day-old embryos and adult mice, we have shown that mouse PI-6 expression is developmentally regulated, and that, unlike human PI-6, it is absent from the placenta [5].
• Northern blot analysis showed a high level of expression of PI-6 mRNA in brain stem and diencephalon as compared with other regions in the adult brain [1].
• In the present study, we investigated the localization of Serpinb6 in the adult mouse brain using in situ hybridization histochemistry and immunohistochemistry [8].
• Two immunoglobulin M monoclonal autoantibodies, PI-6 and AP-2, were found by immunofluorescence, hormone absorption, and enzyme-linked immunosorbent assay to react with insulin and GH, respectively [9].
• The concentration of thrombin in ovalbumin solution (10 mg/mL) could be measured by means of the enzyme-linked immunosorbent assay (ELISA) double sandwich method using the MAb and polyclonal antibody [10].

References