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Gene Review

Shb  -  src homology 2 domain-containing...

Mus musculus

Synonyms: BC028832, SH2 domain-containing adapter protein B
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Disease relevance of Shb

  • Rat insulinoma RINm5F cells overexpressing Shb displayed decreased viability during culture in 0.1% serum and after exposure to a cytotoxic dose of nicotinamide [1].

High impact information on Shb


Biological context of Shb


Anatomical context of Shb


Associations of Shb with chemical compounds

  • We conclude that Shb overexpression is associated with cell degeneration under certain conditions, and that Shb could transduce apoptotic signals from tyrosine kinase receptors [6].
  • The Shb cDNA contains two methionine codons in its N-terminus and thus may code for two proteins of 67 and 56 kDa, each with one SH2 domain in its C-terminus [7].
  • In the present study we have investigated a possible role for the proline-rich SH2 domain protein Shb as a regulator of expression or activity of certain SH3 domain proteins and MAP kinase [9].
  • Transgenic mice overexpressing Shb in beta-cells exhibit an increase in the neonatal beta-cell mass, an improved glucose homeostasis, but also decreased survival in response to cytokines and streptozotocin [10].
  • In beta TC-1 cells, okadaic acid and genistein increased the Shb mRNA content, whereas tyrphostin 25 and serum were without effect [11].

Physical interactions of Shb

  • The data indicate that Shb binds directly to FAK and regulates its phosphorylation leading to enhanced cell spreading in a Src-dependent manner [5].

Regulatory relationships of Shb


Other interactions of Shb

  • Embryoid bodies (EBs) differentiating from Shb-overexpressing ES cells in vitro were stained for CD31 or VEGFR-2 to visualize the formation of vascular structures [4].
  • The addition of platelet-derived growth factor (PDGF-BB) restored the growth of the NIHSHB cells, whereas insulin-like growth factor-1 (IGF-1) failed to affect the proliferation of Shb overexpressing cells in 1% serum [6].
  • However, these effects may partly be caused by altered regulation of Rac1 and Rap1 activation in the Shb cells [12].
  • We observe increased CrkII complex formation with p130(Cas), focal adhesion kinase (FAK), and Shb in PC12-GTK cells [13].
  • The expression of GTK also correlates with a markedly increased content of FAK, phosphorylation of the adaptor protein Shb, and an association between these two proteins [13].

Analytical, diagnostic and therapeutic context of Shb

  • Western blot analysis of the same tissues using an antiserum directed against a synthetic peptide corresponding to a part of the SH2 domain of Shb, revealed reactivity with two proteins of 56 and 67 kDa [7].


  1. Transgenic mice expressing Shb adaptor protein under the control of rat insulin promoter exhibit altered viability of pancreatic islet cells. Welsh, M., Christmansson, L., Karlsson, T., Sandler, S., Welsh, N. Mol. Med. (1999) [Pubmed]
  2. Endostatin-induced tyrosine kinase signaling through the Shb adaptor protein regulates endothelial cell apoptosis. Dixelius, J., Larsson, H., Sasaki, T., Holmqvist, K., Lu, L., Engström, A., Timpl, R., Welsh, M., Claesson-Welsh, L. Blood (2000) [Pubmed]
  3. The Shb adaptor protein binds to tyrosine 766 in the FGFR-1 and regulates the Ras/MEK/MAPK pathway via FRS2 phosphorylation in endothelial cells. Cross, M.J., Lu, L., Magnusson, P., Nyqvist, D., Holmqvist, K., Welsh, M., Claesson-Welsh, L. Mol. Biol. Cell (2002) [Pubmed]
  4. Shb promotes blood vessel formation in embryoid bodies by augmenting vascular endothelial growth factor receptor-2 and platelet-derived growth factor receptor-beta signaling. Rolny, C., Lu, L., Agren, N., Nilsson, I., Roe, C., Webb, G.C., Welsh, M. Exp. Cell Res. (2005) [Pubmed]
  5. The Shb adaptor protein causes Src-dependent cell spreading and activation of focal adhesion kinase in murine brain endothelial cells. Holmqvist, K., Cross, M., Riley, D., Welsh, M. Cell. Signal. (2003) [Pubmed]
  6. Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain protein Shb. Karlsson, T., Welsh, M. Oncogene (1996) [Pubmed]
  7. Shb is a ubiquitously expressed Src homology 2 protein. Welsh, M., Mares, J., Karlsson, T., Lavergne, C., Bréant, B., Claesson-Welsh, L. Oncogene (1994) [Pubmed]
  8. Overexpression of the Shb SH2 domain-protein in insulin-producing cells leads to altered signaling through the IRS-1 and IRS-2 proteins. Welsh, N., Makeeva, N., Welsh, M. Mol. Med. (2002) [Pubmed]
  9. Modulation of Src homology 3 proteins by the proline-rich adaptor protein Shb. Karlsson, T., Welsh, M. Exp. Cell Res. (1997) [Pubmed]
  10. Role of tyrosine kinase signaling for beta-cell replication and survival. Welsh, M., Annerén, C., Lindholm, C., Kriz, V., Oberg-Welsh, C. Ups. J. Med. Sci. (2000) [Pubmed]
  11. Control of SHB gene expression by protein phosphorylation. Lavergne, C., Mares, J., Karlsson, T., Bréant, B., Welsh, M. Cell. Signal. (1996) [Pubmed]
  12. Role of the Src homology 2 domain-containing protein Shb in murine brain endothelial cell proliferation and differentiation. Lu, L., Holmqvist, K., Cross, M., Welsh, M. Cell Growth Differ. (2002) [Pubmed]
  13. GTK, a Src-related tyrosine kinase, induces nerve growth factor-independent neurite outgrowth in PC12 cells through activation of the Rap1 pathway. Relationship to Shb tyrosine phosphorylation and elevated levels of focal adhesion kinase. Annerén, C., Reedquist, K.A., Bos, J.L., Welsh, M. J. Biol. Chem. (2000) [Pubmed]
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