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Gene Review

PLCG1  -  phospholipase C, gamma 1

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Disease relevance of PLCG1

  • Pertussis toxin-insensitive, Gq alpha subunit-mediated IP production via coexpressed M1 muscarinic acetylcholine receptor (M1 AChR) was attenuated only by the PLC gamma PH domain peptide, suggesting that the inhibitory effect of most of the PH domain peptides was G beta gamma subunit-specific [1].

High impact information on PLCG1


Biological context of PLCG1


Anatomical context of PLCG1


Associations of PLCG1 with chemical compounds


Regulatory relationships of PLCG1


Other interactions of PLCG1

  • Here we report the cloning of a bovine brain complementary DNA encoding an enzyme PLC-148 that is characterized by calcium-dependent and phosphatidylinositol-specific phospholipase C activity when expressed in mammalian cells [5].
  • The accompanying manuscript (Kim J.W., Sim, S.S., Kim, U-H., Nishibe, S., Wahl, M. I., Carpenter, G., and Rhe, S. G. (1990) J. Biol. Chem. 265, 3940-3943) identifies these same 2 residues plus 2 additional tyrosine phosphorylation sites through large-scale in vitro phosphorylation of purified bovine brain PLC-gamma by the EGF receptor [17].
  • Tyrosine-phosphorylated Nck, PLC-gamma, and two GAP-associated proteins, p190 and p62, were in GAP immunoprecipitates of VEGF-stimulated BAEC, and tyrosine-phosphorylated NcK was in phosphatidylinositol 3-kinase immunoprecipitates [15].
  • More than 90% of PLC-II was found in the cytosolic fractions, whereas the PLC-I-like molecules were equally distributed between cytosolic and particulate fractions [13].
  • PLC-III exhibited an apparent Mr of 85,000 in NaDodSO4/PAGE, which is considerably smaller than the Mr of 150,000 for PLC-I and 145,000 for PLC-II [18].

Analytical, diagnostic and therapeutic context of PLCG1

  • Western blot analysis confirmed that FACs isolated using RGD-beads were enriched for pp60c-src, pp125FAK, phospholipase C-gamma, and the Na+/H+ antiporter when compared with intact CSK or basal cell surface preparations that retained lipid bilayer [19].
  • Since gel filtration of PLC activity in wild type extracts gave Mr values similar to that of previously characterized PLCs (140,000-200,000), immunoblots with antibodies to bovine brain isoenzymes were used to show that the PLC activities obtained by anion exchange chromatography were PLC-delta and PLC-gamma [8].
  • Spherical monolithic microspheres, with a honeycomb-like internal architecture, composed of PLCG 50:50 and PLCG 75:25 and containing a range of vancomycin loadings, have been fabricated using a W/O emulsification with solvent evaporation technique [20].
  • A second isozyme, PLC-IV, was immunologically distinct from PLC-beta and PLC-gamma and was purified to a stage where three protein bands (Mr 66,000, 61,000 and 54,000) on SDS-PAGE correlated with enzyme activity [21].


  1. Effect of cellular expression of pleckstrin homology domains on Gi-coupled receptor signaling. Luttrell, L.M., Hawes, B.E., Touhara, K., van Biesen, T., Koch, W.J., Lefkowitz, R.J. J. Biol. Chem. (1995) [Pubmed]
  2. Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-gamma 1 complexed with a high affinity binding peptide. Pascal, S.M., Singer, A.U., Gish, G., Yamazaki, T., Shoelson, S.E., Pawson, T., Kay, L.E., Forman-Kay, J.D. Cell (1994) [Pubmed]
  3. Structure of an SH2 domain of the p85 alpha subunit of phosphatidylinositol-3-OH kinase. Booker, G.W., Breeze, A.L., Downing, A.K., Panayotou, G., Gout, I., Waterfield, M.D., Campbell, I.D. Nature (1992) [Pubmed]
  4. Activation of the beta 1 isozyme of phospholipase C by alpha subunits of the Gq class of G proteins. Taylor, S.J., Chae, H.Z., Rhee, S.G., Exton, J.H. Nature (1991) [Pubmed]
  5. Sequence similarity of phospholipase C with the non-catalytic region of src. Stahl, M.L., Ferenz, C.R., Kelleher, K.L., Kriz, R.W., Knopf, J.L. Nature (1988) [Pubmed]
  6. A new tyrosine phosphorylation site in PLC gamma 1: the role of tyrosine 775 in immune receptor signaling. Serrano, C.J., Graham, L., DeBell, K., Rawat, R., Veri, M.C., Bonvini, E., Rellahan, B.L., Reischl, I.G. J. Immunol. (2005) [Pubmed]
  7. The discovery of a 3-phosphomonoesterase that hydrolyzes phosphatidylinositol 3-phosphate in NIH 3T3 cells. Lips, D.L., Majerus, P.W. J. Biol. Chem. (1989) [Pubmed]
  8. Characterization of phosphatidylinositol-specific phospholipase C defects associated with thrombin-induced mitogenesis. Rath, H.M., Fee, J.A., Rhee, S.G., Silbert, D.F. J. Biol. Chem. (1990) [Pubmed]
  9. The tyrosine [correction of tryrosine] phosphorylation and cytoskeletal translocation of phospholipase C gamma 1 in bovine adrenal medullary chromaffin cells. Roberts-Thomson, E.L., Herd, L.M., Saunders, H.I., Dunkley, P.R., Bunn, S.J. Neurochem. Res. (2004) [Pubmed]
  10. Rat parotid gland acinar cell proliferation: signal transduction at the plasma membrane. Purushotham, K.R., Nakagawa, Y., Humphreys-Beher, M.G., Maeda, N., Schneyer, C.A. Crit. Rev. Oral Biol. Med. (1993) [Pubmed]
  11. Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase C gamma 1 in vascular endothelial cells. Venema, V.J., Ju, H., Sun, J., Eaton, D.C., Marrero, M.B., Venema, R.C. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  12. Regulation of phospholipase C gamma 1 activity by GTP-binding proteins in vascular smooth muscle. Hodson, E.A., Ashley, C.C., Lymn, J.S. Biochem. Soc. Trans. (1998) [Pubmed]
  13. Phospholipase C associated with particulate fractions of bovine brain. Lee, K.Y., Ryu, S.H., Suh, P.G., Choi, W.C., Rhee, S.G. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  14. Purification and characterization of two immunologically distinct phosphoinositide-specific phospholipases C from bovine brain. Ryu, S.H., Cho, K.S., Lee, K.Y., Suh, P.G., Rhee, S.G. J. Biol. Chem. (1987) [Pubmed]
  15. Vascular endothelial cell growth factor promotes tyrosine phosphorylation of mediators of signal transduction that contain SH2 domains. Association with endothelial cell proliferation. Guo, D., Jia, Q., Song, H.Y., Warren, R.S., Donner, D.B. J. Biol. Chem. (1995) [Pubmed]
  16. Lysophosphatidylcholine inhibits endothelial cell migration and proliferation via inhibition of the extracellular signal-regulated kinase pathway. Rikitake, Y., Kawashima, S., Yamashita, T., Ueyama, T., Ishido, S., Hotta, H., Hirata, K., Yokoyama, M. Arterioscler. Thromb. Vasc. Biol. (2000) [Pubmed]
  17. Identification of two epidermal growth factor-sensitive tyrosine phosphorylation sites of phospholipase C-gamma in intact HSC-1 cells. Wahl, M.I., Nishibe, S., Kim, J.W., Kim, H., Rhee, S.G., Carpenter, G. J. Biol. Chem. (1990) [Pubmed]
  18. Bovine brain cytosol contains three immunologically distinct forms of inositolphospholipid-specific phospholipase C. Ryu, S.H., Suh, P.G., Cho, K.S., Lee, K.Y., Rhee, S.G. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  19. Convergence of integrin and growth factor receptor signaling pathways within the focal adhesion complex. Plopper, G.E., McNamee, H.P., Dike, L.E., Bojanowski, K., Ingber, D.E. Mol. Biol. Cell (1995) [Pubmed]
  20. Incorporation and release of vancomycin from poly(D,L-lactide-co-glycolide) microspheres. Atkins, T.W., Peacock, S.J., Yates, D.J. Journal of microencapsulation. (1998) [Pubmed]
  21. Purification and characterization of PLC-beta m, a muscarinic cholinergic regulated phospholipase C from rabbit brain membrane. Carter, H.R., Wallace, M.A., Fain, J.N. Biochim. Biophys. Acta (1990) [Pubmed]
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