Disease relevance of IGKV1-39

• Among 916 Pseudomonas aeruginosa strains isolated from children in a Warsaw hospital during the period 1985-1988 the most frequently encountered serotypes were O6, O11, O12, and O16 [1].
• There was a greater than 10(5) difference in LD50 in newborn rats between K1-positive and K1-negative pairs of E. coli serotypes O18 and O7 and a greater than 1 log difference between isogenic pairs of serotype O12; however, the K5 isogenic pairs had a similar LD50 [2].
• The limits of typing methods for the investigation of outbreaks of nosocomial infection with multi-resistant P. aeruginosa O12 are emphasized [3].
• Eleven of the 45, O12 isolates were resistant to all phages, and 31 were sensitive only to phage 68 [4].

High impact information on IGKV1-39

• CHEB, TRE, and TRO LC genes were found to be highly homologous to the same germline gene O2/O12 [5].
• However, no such difference was observed between a highly virulent S. enterica serovar Enteritidis strain, 7314 (somatic antigen, O1, O9, O12; flagellum antigen phase 1, g,m; flagellum antigen phase 2 [1,7]), and its SefA-deficient isogenic mutant [6].
• The pattern A isolates were mostly of serotype O12 and were highly resistant to imipenem (MICs, 32 to >256 microg/ml), with this resistance decreased eightfold or more in the presence of EDTA [7].
• Of the 18 strains of V. cholerae isolated during this period, 15 belonged to the non-O1, non-O139 serogroups (4 belonged to O144, 3 belonged to O11, 1 each belonged to O6, O8, O12, O19, O39, and O58, and 2 strains could not be typed), 2 belonged to the O139 serogroup, and 1 belonged to the O1 serogroup [8].
• All strains were characterized by antibiotic resistance pattern analysis, beta-lactamase content analysis, plasmid profiling, ribotyping with EcoRI, and arbitrarily primed (AP)-PCR with primers O8 and O12 [9].

Biological context of IGKV1-39

• Most O12 isolates had a penicillinase-producing phenotype and were resistant to aminoglycosides and ciprofloxacin, but susceptible to fosfomycin [10].
• No significant difference in virulence was shown between serogroup O10 and O12 isolates [11].
• To systematically investigate the relationship between the amino acid sequence and V(H)/V(L) interaction strength, we here used a set of anti-bovine serum albumin antibodies having a single human framework for V(H) (V3-23/DP-47 and JH4b) and Vk (O12/O2/DPK9 and Jk1), but with different V(H)/V(L) interaction strengths [12].
• Genotypic homogeneity of nosocomial Pseudomonas aeruginosa O12 strains demonstrated by analysis of protein profiles, DNA fingerprints and rRNA gene restriction patterns [13].

Anatomical context of IGKV1-39

• Among a group of O10 and O12 isolates selected for virulence studies, none produced enterotoxin whereas the majority produced a cytotoxin, as assessed in Y1 and HeLa cells [11].
• In summary, these data show the high frequency utilization of the germline O2/O12 gene and a high rate of mutation with an evidence of antigen selection in most of the Ig kappa genes expressed in the RA synovium [14].

Associations of IGKV1-39 with chemical compounds

• Structure II ([AlLH2(OH)(H2O)]2+) has the metal coordinated to the O11 and O12 groups and the O3 group protonated and is the global minimum on the potential energy surface for the interaction [15].
• Resistance to gentamicin, tobramycin, amikacin, imipenem and ciprofloxacin was more frequent among strains belonging to serotype O12 [16].
• The mutational frequency of serotype O12 was similar to that of other serotypes and thus could not explain the susceptibility to fosfomycin [17].
• The IR and Raman spectra of polycrystalline anhydrous orotic acid and its N1, N3, and O12 trideuterated isotopomer are recorded in the 4000-40 cm(-1) spectral interval as part of a series of vibrational analyses of nucleosides, nucleotides, and related compounds carried out in our laboratory [18].
• However, in this study ribotyping with EcoRI and PvuII distinguished seven clones among 24 ticarcillin resistant serotype O12 isolates, although one ribotype predominated (67%) [17].

Other interactions of IGKV1-39

• Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21 (12.5%), O8 (8.7%), O6/O7 (5.8%), O4 (3.8%), O18 (2.9%), and O9 (2.9%) [19].

Analytical, diagnostic and therapeutic context of IGKV1-39

• Strains were typed by competitive enzyme-linked immunosorbent assay with rabbit antisera specific for serogroups O1 to O12 and monoclonal antibodies (MAbs) specific for serogroups O1, O2ab, O2ac, and the genus-specific core antigen [20].
• Among the eight C. diversus strains, strains Cd5 to Cd12, six isolates (isolates Cd6 to Cd11) were identical by all markers; one strain (strain Cd5) differed by two markers (antibiotype and AP-PCR pattern with primer O8), and the remaining strain (strain Cd12) differed by two other markers (ribotype and AP-PCR pattern with primer O12) [9].
• During 2003, when the environmental study was undertaken, serotype O12 isolates with bla(VIM) were recovered from sinks and stethoscopes in the most-affected units, although not from the hands of staff; the problem declined once these reservoirs were disinfected and hygienic precautions were reinforced [7].
• Pseudomonas aeruginosa serotype O12 outbreak studied by arbitrary primer PCR [21].
• The serotyping and antibiotic susceptibility patterns suggested a few endemic strains of serotypes O13, O2/3, O12/14, and nontypable strains [22].

References