Disease relevance of pcna

• The mutant lens and retina also displayed increased cell proliferation based on proliferating cell nuclear antigen immunolabeling, suggesting that the lens opacity was due to unregulated cell proliferation and undifferentiated cell accumulation within the mutant lens [1].

High impact information on pcna

• This study reveals that the early larval stage in Xenopus (Stage 48) displays patterns of proliferation (PCNA), as well as of neurogenic (Delta-1) and proneural (Ngnr-1; NeuroD) gene expression that are qualitatively almost identical to those seen in the 3-day postembryonic zebrafish or the 12.5/13.5-day embryonic mouse [2].
• High levels of cell proliferation within the inner nuclear layer (INL) were observed within the first 3 days of constant light, as assessed by immunodetection of proliferating cell nuclear antigen and BrdU labeling [3].
• In addition, opsin immunohistochemistry and TUNEL were used to compare photoreceptor cell death in these different retinal areas, while PCNA immunolabeling quantified the cell proliferation that precedes the photoreceptor regeneration [4].
• In chordin morphants, PCNA is significantly upregulated in the expanded ICM [5].
• PCNA was also expressed in spermatogonial stem cells and intestine crypts, consistent with its role in cell proliferation and DNA synthesis [5].

Biological context of pcna

• Evaluation of cell proliferation was performed in proliferating cell nuclear antigen (PCNA)-labeled tissue sections as well as in single-cell suspensions analyzed by flow cytometry [6].
• We investigated the expression of proliferative cell nuclear antigen (PCNA) in zebrafish to delineate the proliferative hematopoietic component during adult and embryonic hematopoiesis [5].
• Specifically, the ef1-a:EGFP transgene colabels with PCNA in the M??ller glia, a specialized cell that is the source of neuronal progenitors during zebrafish retinal regeneration [7].
• Furthermore, PCNA was found to be present in nuclei of oogonia and oocytes in meiotic prophase [8].
• Triple uki/dre/lep mutants show neither an additive increase in PCNA expression nor enhanced embryonic phenotypes, suggesting that other negative regulators, possibly Ptc1, prevent further activation of the Hh signaling pathway [9].

Anatomical context of pcna

• Immunostaining for PCNA and enhanced green fluorescence protein (eGFP) was performed in wild-type and fli1-eGFP (endothelial marker) and gata1-eGFP (erythroid cell marker) transgenic fish [5].
• In embryos, PCNA is expressed in the brain, spinal cord and intermediate cell mass (ICM) at 24 h-post fertilization [5].
• PCNA was expressed in hematopoietic progenitor cells but not in mature neutrophils, eosinophils or erythroid cells [5].
• In degenerating oocytes, over 60% of the former follicle cells were positive for PCNA [8].
• PCNA, a nuclear protein intimately involved in DNA synthesis, has been localized immunohistochemically in ovaries of zebrafish (Brachydanio rerio, Ham.-Buch.). Positively stained cells were detected in the follicle epithelium of all stages of growing oocytes and degenerating oocytes [8].

Other interactions of pcna

• Additionally, double-immunolabel preparations for visualizing TH cells and cells containing the proliferating cell nuclear antigen (PCNA cells) were carried out allowing for a prosomeric interpretation of early forebrain TH cell clusters [10].
• This fact as well as comparisons with PCNA- and Hu-immunostains indicate that a great proportion of ngn1-positive cells are mitotic, but some appear to extend into the postmitotic gray matter where the nrd-domains lie [11].

References