Psychiatry related information on Jhe

• It is also known that both the insulin pathway and JH play a role in the control of sexual dimorphism in locomotor activity [1].

High impact information on Jhe

• In fluorescence-based binding assays, USP protein binds JH III and JH III acid with specificity, adopting for each ligand a different final conformational state [2].
• Identification and characterization of a juvenile hormone (JH) response region in the JH esterase gene from the spruce budworm, Choristoneura fumiferana [3].
• The lowered levels of rRNA ultimately would limit the concentration of ribosomes needed to produce large quantities of a protein (in these cases, juvenile hormone esterase) needed for normal development [4].
• Localization of a Drosophila melanogaster homolog of the putative juvenile hormone esterase binding protein of Manduca sexta [5].
• The function of P29 in mitochondria and the possible interaction with JHE are discussed [5].

Biological context of Jhe

• It was shown that attainment of a definite JHE activity level in females of lines 101 and 147 agrees well with the onset of oviposition of fertilized eggs [6].
• Binding in the active site of JHE is promoted by structural features found in JHIII and JHB3 including the epoxide groups in their natural orientations, methyl (rather than ethyl) side-chains, and the 2E, 3 double bond that is conjugated with the ester group [7].
• Competition of JHIII hydrolysis by five juvenile hormones and twenty-four JH analogues showed JHE is highly selective for JHIII and JHIII bisepoxide (JHP3), and both may be in vivo substrates [7].
• Identification of a juvenile hormone esterase gene by matching its peptide mass fingerprint with a sequence from the Drosophila genome project [8].
• This predicted JHE sequence includes features that are conserved among all active members of the serine carboxylesterase multigene family as well as features peculiar to JHEs from other species [8].

Anatomical context of Jhe

• Similarly to the InR, HMGCR is expressed in the corpus allatum (ca), which is the gland where JH biosynthesis occurs [1].

Associations of Jhe with chemical compounds

• The same DR12 element conferred enhanced transcriptional responsiveness of a transfected juvenile hormone esterase core promoter to treatment of transfected cells with JH III, but not to treatment with retinoic acid or T3 [9].
• JHE also hydrolyses the artificial substrate alpha-naphthyl acetate with a KM of 120 micro M and a Vmax of at least 70 mumol/min/mg [7].
• Tyrosine decarboxylase activity in males and females of the LA line was sharply reduced as compared with those of the HA and control Canton-S lines; JH-esterase and JH-epoxide hydrolase activities were decreased in females, and not in males, of the LA line [10].
• The metabolism of juvenile hormone by JH-esterase and JH-epoxide hydrolase, and octopamine by tyrosine decarboxylase were studied under normal and stress conditions in flies of two related lines of D. melanogaster [10].
• In insects, which can not synthesize cholesterol de novo, the HMGCR is implicated in the biosynthesis of juvenile hormone (JH) [1].

Regulatory relationships of Jhe

• JH III treatment of Saccharomyces cerevisiae expressing a LexA-USP fusion protein stabilizes an oligomeric association containing this protein, as detected by formation of a protein-DNA complex, and induces USP-dependent transcription in a reporter assay [2].

Other interactions of Jhe

• Differences in the course of changes in the JHE activity level between adults of lines 101 and 147 of D. virilis were found, and also in the JHEH activity level between adults of lines Canton S and 921283 of D. melanogaster [6].
• EcRB1 is normally induced with a form of USP that is missing its DNA-binding domain (DBD), although potentiation by juvenile hormone (JH) III is reduced [11].
• At metamorphosis in the absence of JH, a new isoform of EcR and some new ecdysteroid-induced regulatory factors appear in response to low ecdysteroid [12].

Analytical, diagnostic and therapeutic context of Jhe

• Juvenile hormone esterase (JHE) from the prepupal stage of Drosophila melanogaster was purified about 429-fold to near homogeneity by selective precipitations, isoelectric focussing, anion exchange and gel filtration chromatography [7].
• Also we show that this JHE can be purified by an alternative method using anion exchange chromotography followed by trifluoromethylketone affinity chromatography [8].
• Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to study developmental expression and hormonal regulation of the juvenile hormone esterase gene (DmJhe) in the fruit fly, Drosophila melanogaster [13].
• JHE bands from dried silver-stained SDS-PAGE gels of that material were digested with trypsin [8].
• The content of JH-esterase was assayed by radial immunodiffusion in Drosophila virilis pupae under normal conditions and under the effects of extreme factors [14].

References