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MeSH Review


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Disease relevance of Immunodiffusion


High impact information on Immunodiffusion


Chemical compound and disease context of Immunodiffusion


Biological context of Immunodiffusion

  • This result was confirmed by an immunodiffusion test that demonstrated the absence of production of the C4 protein in mice of haplotype H-2aw18 [16].
  • The CA II protein, which is normally expressed in most tissues, could not be detected by immunodiffusion analysis in any tissues of the CA II-deficient mice, suggesting a nonsense or a missense mutation at the Car-2 locus [17].
  • The immunodiffusion and hemagglutination inhibition tests also failed to demonstrate that the capsular material (polyribose phosphate) was in ribosomal preparations [18].
  • EGF stimulated phosphorylation of keratin proteins (Mr: 65,000, 60,000, 56,000, and 51,000) identified by the Ouchterlony immunodiffusion analysis, a low Mr protein (16,000 dalton) of the urea-SDS-mercaptoethanol soluble fraction, and a 30,000 dalton Tris-HCl soluble protein [19].
  • The results suggest that a single binding site is present on HSA: the apparent size of the HSA-protein G complex (230 kDa) corresponded to two or three HSA molecules bound to one protein G molecule, and Ouchterlony immunodiffusion did not yield any precipitate between protein G and HSA [20].

Anatomical context of Immunodiffusion


Associations of Immunodiffusion with chemical compounds

  • Radial double immunodiffusion experiments using antiserum to C1q, C1r, and C1s on HFf-treated serum demonstrated the dissociation of the C1 trimolecular complex, with concomitant reduction of C1r antigenicity that is indicative of C1 activation [26].
  • C3b inactivator (C3bINA) has been measured in biologic fluids by radial immunodiffusion using a monospecific antiserum prepared in rabbits, and by a hemolytic assay which measures the reduction in the capacity of EAC43 cells bearing limited C3b sites to form C3B, the alternative pathway C3 convertase [1].
  • With the exception of a moderately reduced homolytic C4, all other C components, measured homolytically and by radial immunodiffusion, were present in normal amounts [27].
  • Histone H5 was demonstrated in all samples of these cells and was characterized by electrophoresis in polyacrylamide gel, extraction with perchloric acid, amino acid analysis, and immunodiffusion in agarose gel [28].
  • Upper and lower phases and serial fractions were analyzed for vitamin D3 (extraction, HPLC), cholesterol (enzyme assay), and human DBP (hDBP) (radial immunodiffusion) [29].

Gene context of Immunodiffusion

  • C-reactive protein (CRP) was assayed by radial immunodiffusion [30].
  • Double immunodiffusion precipitation revealed that ALDH1 and ALDH2 were immunologically analogous but not identical, and that CRM and ALDH2 were immunologically indistinguishable [31].
  • Physical association of apoA-I, LBP, and FHRP in these particles was further confirmed using double immunodiffusion, and association of LBP and FHRP in plasma was confirmed by coimmunoprecipitation [32].
  • Hex A, the major form in adults, and Hex B, the predominant form in larvae, showed complete immunological identity by double immunodiffusion and enzyme immunoinactivation studies [33].
  • Antiserum to the liver HO-1 did not recognize the testicular HO-2 when examined by double immunodiffusion or by Western immunoblotting [34].

Analytical, diagnostic and therapeutic context of Immunodiffusion


  1. C3b inactivator in the rheumatic diseases. Measurement by radial immunodiffusion and by inhibition of formation of properdin pathway C3 convertase. Whaley, K., Schur, P.H., Ruddy, S. J. Clin. Invest. (1976) [Pubmed]
  2. Shared interspecies antigenic reactivities among hamster and feline oncoviruses. Jerabek, L.B., Gilbert, J.H., Sanders, F.K., Hardy, W.D. J. Natl. Cancer Inst. (1981) [Pubmed]
  3. Intestinal clearance of alpha 1-antitrypsin. A sensitive method for the detection of protein-losing enteropathy. Florent, C., L'Hirondel, C., Desmazures, C., Aymes, C., Bernier, J.J. Gastroenterology (1981) [Pubmed]
  4. Autoantibodies and the spectrum of Sjögren's syndrome. Martinez-Lavin, M., Vaughan, J.H., Tan, E.M. Ann. Intern. Med. (1979) [Pubmed]
  5. Immunodiagnosis of aspergillosis. Kurup, V.P., Kumar, A. Clin. Microbiol. Rev. (1991) [Pubmed]
  6. X-linked cutis laxa: defective cross-link formation in collagen due to decreased lysyl oxidase activity. Byers, P.H., Siegel, R.C., Holbrook, K.A., Narayanan, A.S., Bornstein, P., Hall, J.G. N. Engl. J. Med. (1980) [Pubmed]
  7. Hypertriglyceridemia associated with deficiency of apolipoprotein C-II. Breckenridge, W.C., Little, J.A., Steiner, G., Chow, A., Poapst, M. N. Engl. J. Med. (1978) [Pubmed]
  8. Glutamine-stimulated modification and degradation of glutamine synthetase in hepatoma tissue culture cells. Arad, G., Freikopf, A., Kulka, R.G. Cell (1976) [Pubmed]
  9. Antibody to spermine: a natural biological constituent. Bartos, D., Bartos, F., Campbell, R.A., Grettie, D.P., Smejtek, P. Science (1980) [Pubmed]
  10. Influenza-associated myoglobinuric renal failure. Cunningham, E., Kohli, R., Venuto, R.C. JAMA (1979) [Pubmed]
  11. Cull rates of dairy cattle with antibodies to bovine leukemia virus. Thurmond, M.C., Maden, C.B., Carter, R.L. Cancer Res. (1985) [Pubmed]
  12. Elevation of the placental glutathione S-transferase form (GST-pi) in tumor tissues and the levels in sera of patients with cancer. Tsuchida, S., Sekine, Y., Shineha, R., Nishihira, T., Sato, K. Cancer Res. (1989) [Pubmed]
  13. Increased plasma IgA, sIgA, and C3- and IgA-containing immune complexes with renal glomerular deposits in diabetic rats. Miller, L.L., Izzo, M.J., Wemett, D., Panner, B.J., Schenk, E.A. Diabetes (1988) [Pubmed]
  14. Serologic monitoring of disease and treatment in a patient with pulmonary aspergilloma. Tomee, J.F., van der Werf, T.S., Latge, J.P., Koeter, G.H., Dubois, A.E., Kauffman, H.F. Am. J. Respir. Crit. Care Med. (1995) [Pubmed]
  15. Lipopolysaccharide-derived serotype polysaccharides from Neisseria meningitidis group B. Apicella, M.A. J. Infect. Dis. (1979) [Pubmed]
  16. Lethal deletion of the complement component C4 and steroid 21-hydroxylase genes in the mouse H-2 class III region, caused by meiotic recombination. Shiroishi, T., Sagai, T., Natsuume-Sakai, S., Moriwaki, K. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  17. N-ethyl-N-nitrosourea-induced null mutation at the mouse Car-2 locus: an animal model for human carbonic anhydrase II deficiency syndrome. Lewis, S.E., Erickson, R.P., Barnett, L.B., Venta, P.J., Tashian, R.E. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  18. Characterization of the immunoprotective antigen of ribosomal preparations from Haemophilus influenzae. Tewari, R.P., Lynn, M., Birnbaum, A.J., Solotorovsky, M. Infect. Immun. (1978) [Pubmed]
  19. Epidermal growth factor stimulates phosphorylation of pig epidermal keratin protein. Aoyagi, T., Umeda, K., Kato, N., Fukaya, T., Kobayashi, H., Koizumi, H., Miura, Y. J. Invest. Dermatol. (1983) [Pubmed]
  20. Localization of the binding site for streptococcal protein G on human serum albumin. Identification of a 5.5-kilodalton protein G binding albumin fragment. Falkenberg, C., Björck, L., Akerström, B. Biochemistry (1992) [Pubmed]
  21. Rapid diagnosis of thyroid disease using carbonic-anhydrase immunoassay. Auton, J.A., Barragry, J.M., Carter, N.D., Morris, D.V., Cohen, R.D. Lancet (1976) [Pubmed]
  22. Human embryonic prealbumin as a marker for tumours and fibroblasts. Tatarinov, Y.S., Kalashnikov, V.V., Vasiliev, M.Y., Voloshuk, S.G., Kraevsky, N.A., Vorsanova, S.G. Lancet (1978) [Pubmed]
  23. Immunocytochemical localization of the lens main intrinsic polypeptide (MIP26) in communicating junctions. Bok, D., Dockstader, J., Horwitz, J. J. Cell Biol. (1982) [Pubmed]
  24. Rat intestinal brush border membrane peptidases. I. Solubilization, purification, and physicochemical properties of two different forms of the enzyme. Kim, Y.S., Brophy, E.J. J. Biol. Chem. (1976) [Pubmed]
  25. Human granulocyte-specific nuclear antigen(s). II. Detection of antigens in human proliferative syndromes. Briggs, R.C., Forbes, J.T., Hnilica, L.S., Montiel, M.M., Thor, D.E. J. Immunol. (1980) [Pubmed]
  26. Activation of the classical pathway of complement by Hageman factor fragment. Ghebrehiwet, B., Silverberg, M., Kaplan, A.P. J. Exp. Med. (1981) [Pubmed]
  27. Complete absence of the third component of complement in man. Ballow, M., Shira, J.E., Harden, L., Yang, S.Y., Day, N.K. J. Clin. Invest. (1975) [Pubmed]
  28. Histone H5 in the immature blood cells of chickens with leukosis induced by avian leukosis virus strain E26. Sotirov, N. J. Natl. Cancer Inst. (1981) [Pubmed]
  29. Human plasma transport of vitamin D after its endogenous synthesis. Haddad, J.G., Matsuoka, L.Y., Hollis, B.W., Hu, Y.Z., Wortsman, J. J. Clin. Invest. (1993) [Pubmed]
  30. Serum cytokine profiles in experimental human malaria. Relationship to protection and disease course after challenge. Harpaz, R., Edelman, R., Wasserman, S.S., Levine, M.M., Davis, J.R., Sztein, M.B. J. Clin. Invest. (1992) [Pubmed]
  31. Isolation and characterization of aldehyde dehydrogenase isozymes from usual and atypical human livers. Ikawa, M., Impraim, C.C., Wang, G., Yoshida, A. J. Biol. Chem. (1983) [Pubmed]
  32. Plasma lipopolysaccharide-binding protein is found associated with a particle containing apolipoprotein A-I, phospholipid, and factor H-related proteins. Park, C.T., Wright, S.D. J. Biol. Chem. (1996) [Pubmed]
  33. Multiple forms of Drosophila hexokinase. Purification, biochemical and immunological characterization. Moser, D., Johnson, L., Lee, C.Y. J. Biol. Chem. (1980) [Pubmed]
  34. Purification and characterization of the major constitutive form of testicular heme oxygenase. The noninducible isoform. Trakshel, G.M., Kutty, R.K., Maines, M.D. J. Biol. Chem. (1986) [Pubmed]
  35. Production of specific antisera and monoclonal antibodies to choline acetyltransferase: characterization and use for identification of cholinergic neurons. Eckenstein, F., Thoenen, H. EMBO J. (1982) [Pubmed]
  36. Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells. Starger, J.M., Brown, W.E., Goldman, A.E., Goldman, R.D. J. Cell Biol. (1978) [Pubmed]
  37. Involucrin synthesis is correlated with cell size in human epidermal cultures. Watt, F.M., Green, H. J. Cell Biol. (1981) [Pubmed]
  38. Serology of coccidioidomycosis. Pappagianis, D., Zimmer, B.L. Clin. Microbiol. Rev. (1990) [Pubmed]
  39. Identification, purification, and characterization of a non-heme lactoperoxidase in bovine milk. Dumontet, C., Rousset, B. J. Biol. Chem. (1983) [Pubmed]
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