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Gene Review

mAChR-A  -  muscarinic Acetylcholine Receptor, A-type

Drosophila melanogaster

Synonyms: Acr60C, AcrC, CG4356, DM1, Dm-mAChR-A, ...
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Disease relevance of mAcR-60C

  • Pretreatment of S2-DM1-TRPL cells with 10 microM atropine abolished Gd(3+)-insensitive Ca2+ influx triggered by carbamylcholine, but the response was not blocked by prior incubation with pertussis toxin [1].
  • To test this hypothesis, Ca2+ signalling was examined in Fura-2-loaded Sf9 cells infected with baculovirus containing cDNA for the M5 muscarinic receptor alone (M5 cells) or in cells co-infected with both M5 and Trpl-containing baculoviruses (M5-Trpl cells) [2].

High impact information on mAcR-60C


Biological context of mAcR-60C

  • The muscarinic receptor maps to the tip of the right arm of the second chromosome of the Drosophila genome [7].
  • The staining of a discrete group of neurosecretory cells in the pars intercerebralis of the brain indicates a possible new role for this mAChR in the regulation of neurosecretion [8].
  • Other evidence for muscarinic receptor subtypes which is examined includes: heterogeneous responses to classical muscarinic ligands and evidence for coupling of invertebrate muscarinic receptors to several different classes of second messenger systems [9].
  • The nematode mAChR, consisted of 585 amino acids, displays a high degree of amino acid sequence homology to other invertebrate and vertebrate mAChRs [10].
  • Comparison of the cDNA sequence with the corresponding genomic sequence reveals that the C. elegans mAChR gene contains ten introns, eight of them in the coding region [10].

Anatomical context of mAcR-60C

  • We have stably co-expressed Drosophila TRPL with a Drosophila muscarinic acetylcholine receptor (DM1) in a Drosophila cell line (S2 cells) [1].
  • Pharmacological profiles of the C. elegans mAChR expressed in Chinese hamster ovary (CHO) cells were shown to be similar to those of mammalian counterparts, indicating that ligand binding domains of the receptor have been conserved during evolution [10].
  • Muscarinic receptors in brain membranes from honey bees, houseflies, and the American cockroach were identified by their specific binding of the non-selective muscarinic receptor antagonist [3H]quinuclidinyl benzilate ([3H]QNB) and the displacement of this binding by agonists as well as subtype-selective antagonists, using filtration assays [11].

Associations of mAcR-60C with chemical compounds


Regulatory relationships of mAcR-60C

  • A cloned Drosophila muscarinic acetylcholine receptor (mAChR) has been stably expressed in a Drosophila cell line (S2) under the control of an inducible Drosophila metallothionein promoter [16].

Other interactions of mAcR-60C


  1. Thapsigargin and receptor-mediated activation of Drosophila TRPL channels stably expressed in a Drosophila S2 cell line. Yagodin, S., Hardie, R.C., Lansdell, S.J., Millar, N.S., Mason, W.T., Sattelle, D.B. Cell Calcium (1998) [Pubmed]
  2. Receptor-mediated activation of recombinant Trpl expressed in Sf9 insect cells. Hu, Y., Schilling, W.P. Biochem. J. (1995) [Pubmed]
  3. Characterization and functional expression in mammalian cells of genomic and cDNA clones encoding a Drosophila muscarinic acetylcholine receptor. Shapiro, R.A., Wakimoto, B.T., Subers, E.M., Nathanson, N.M. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  4. Drosophila nervous system muscarinic acetylcholine receptor: transient functional expression and localization by immunocytochemistry. Blake, A.D., Anthony, N.M., Chen, H.H., Harrison, J.B., Nathanson, N.M., Sattelle, D.B. Mol. Pharmacol. (1993) [Pubmed]
  5. Adaptive evolution of G-protein coupled receptor genes. Yokoyama, S., Isenberg, K.E., Wright, A.F. Mol. Biol. Evol. (1989) [Pubmed]
  6. Gene silencing of selected calcium-signalling molecules in a Drosophila cell line using double-stranded RNA interference. Raymond-Delpech, V., Towers, P.R., Sattelle, D.B. Cell Calcium (2004) [Pubmed]
  7. Cloning, sequence analysis and chromosome localization of a Drosophila muscarinic acetylcholine receptor. Onai, T., FitzGerald, M.G., Arakawa, S., Gocayne, J.D., Urquhart, D.A., Hall, L.M., Fraser, C.M., McCombie, W.R., Venter, J.C. FEBS Lett. (1989) [Pubmed]
  8. Localization in the nervous system of Drosophila melanogaster of a C-terminus anti-peptide antibody to a cloned Drosophila muscarinic acetylcholine receptor. Harrison, J.B., Chen, H.H., Blake, A.D., Huskisson, N.S., Barker, P., Sattelle, D.B. J. Neuroendocrinol. (1995) [Pubmed]
  9. Muscarinic acetylcholine receptors in invertebrates: comparisons with homologous receptors from vertebrates. Hannan, F., Hall, L.M. EXS. (1993) [Pubmed]
  10. Cloning and functional characterization of a Caenorhabditis elegans muscarinic acetylcholine receptor. Hwang, J.M., Chang, D.J., Kim, U.S., Lee, Y.S., Park, Y.S., Kaang, B.K., Cho, N.J. Recept. Channels (1999) [Pubmed]
  11. Pharmacologic characterization of muscarinic receptors of insect brains. Abdallah, E.A., Eldefrawi, M.E., Eldefrawi, A.T. Arch. Insect Biochem. Physiol. (1991) [Pubmed]
  12. Muscarinic receptor in Drosophila melanogaster demonstrated by binding of [3H]quinuclidinyl benzilate. Dudai, Y., Ben-Barak, J. FEBS Lett. (1977) [Pubmed]
  13. Functional characterization of thapsigargin and agonist-insensitive acidic Ca2+ stores in Drosophila melanogaster S2 cell lines. Yagodin, S., Pivovarova, N.B., Andrews, S.B., Sattelle, D.B. Cell Calcium (1999) [Pubmed]
  14. Characterization of muscarinic binding sites in the central nervous system of larval Manduca sexta. Qazi, S., Proulx, D., Trimmer, B.A. Insect Biochem. Mol. Biol. (1996) [Pubmed]
  15. Spatiotemporal calcium signaling in a Drosophila melanogaster cell line stably expressing a Drosophila muscarinic acetylcholine receptor. Cordova, D., Delpech, V.R., Sattelle, D.B., Rauh, J.J. Invert. Neurosci. (2003) [Pubmed]
  16. Functional expression of a cloned Drosophila muscarinic acetylcholine receptor in a stable Drosophila cell line. Millar, N.S., Baylis, H.A., Reaper, C., Bunting, R., Mason, W.T., Sattelle, D.B. J. Exp. Biol. (1995) [Pubmed]
  17. Activation of heterologously expressed Drosophila TRPL channels: Ca2+ is not required and InsP3 is not sufficient. Hardie, R.C., Raghu, P. Cell Calcium (1998) [Pubmed]
  18. Temporal and spatial expression patterns of two G-protein coupled receptors in Drosophila melanogaster. Hannan, F., Hall, L.M. Invert. Neurosci. (1996) [Pubmed]
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