Disease relevance of TPI1

• Chicken triosephosphate isomerase complements an Escherichia coli deficiency [1].
• Screening a chicken genomic library with cDNA coding for triosephosphate isomerase led to the isolation of phage containing the entire gene, which we used to map the transcriptional start [1].
• Its deduced amino acid sequence revealed 34% identity with the thermophilic bacteria Bacillus stearothermophilus TIM [2].
• The TIM gene from psychrophilic bacteria Moraxella sp. TA137 was cloned and its nucleotide sequence determined [2].
• For example, the deamidated isoforms of triosephosphate isomerase accumulate in: (a) old erythrocytes, (b) fibroblasts from old donors, (c) fibroblasts aged in vitro, (d) premature-aging syndromes and (e) old cells in the eye lens [3].

High impact information on TPI1

• The triosephosphate isomerase gene from maize: introns antedate the plant-animal divergence [4].
• Structure of chicken muscle triose phosphate isomerase determined crystallographically at 2.5 angstrom resolution using amino acid sequence data [5].
• Simulations of the folding pathways of two large alpha/beta proteins, the alpha subunit of tryptophan synthase and triose phosphate isomerase, are reported using the knight's walk lattice model of globular proteins and Monte Carlo dynamics [6].
• We present the sequence of full-length chicken triosephosphate isomerase (D-glyceraldehyde 3-phosphate ketol-isomerase, EC 5.3.1.1) mRNA based on the analysis of cDNA and genomic clones [1].
• We have replaced the glutamic acid-165 at the active site of chicken triosephosphate isomerase with an aspartic acid residue using site-directed mutagenesis [7].

Anatomical context of TPI1

• A hybrid protein that comprises the beta-lactamase signal peptide fused precisely to chicken muscle triosephosphate isomerase is not secreted into the periplasm of Escherichia coli [8].
• These clusters may be involved in the mechanism of import of this triosephosphate isomerase into the glycosome [9].

Associations of TPI1 with chemical compounds

• Glycine is never observed in native TIM hinge sequences [10].
• The N-terminal hinge was mutated to P166/V167G/W168G (PGG), and the C-terminal hinge was mutated to K174G/T175G/A176G (GGG) in chicken TIM [10].
• The crystal structure of recombinant chicken triosephosphate isomerase (TIM, E.C. 5.3.1.1) complexed with the intermediate analogue phosphoglycolohydroxamate (PGH) has been solved by the method of molecular replacement and refined to an R-factor of 18.5% at 1.8-A resolution [11].
• Studies of the histidine residues of triose phosphate isomerase by proton magnetic resonance and x-ray crystallography [12].
• The infrared spectrum of dihydroxyacetone phosphate bound to triosephosphate isomerase has been measured [13].

Other interactions of TPI1

• These included aldolase, ribulose-1,5-bisphosphate carboxylase/oxygenase, ribonuclease A, carbonic anhydrase and triosephosphate isomerase [14].

Analytical, diagnostic and therapeutic context of TPI1

• Genetic engineering in the Precambrian: structure of the chicken triosephosphate isomerase gene [15].
• Rapid isolation of triosephosphate isomerase utilizing high-performance liquid chromatography [16].

References