Disease relevance of Lens, Crystalline

• In several tissues the amount of hsp25 exceeds that accumulating in NIH 3T3 fibroblasts in response to heat stress. hsp25, like alpha B crystallin, exists in a highly aggregated form in the eye lens [1].
• Its aerobic photoreactivity and adverse effects on antioxidant activity combined with its gradual accumulation over time suggests that its in vivo phototoxicity increases with age despite changes in the absorption characteristics of the crystalline lens [2].
• Protein S, a Ca(2+)-binding spore-coat protein from the soil bacterium Myxococcus xanthus exhibits a high degree of sequential and structural homology with gammaB-crystallin from the vertebrate eye lens [3].
• Heterologous expression in Escherichia coli of native and mutant forms of the major intrinsic protein of rat eye lens (MIP26) [4].
• The anterior shift of the lens-iris diaphragm caused by ciliochoroidal effusion with ciliary body edema and thickening of the crystalline lens from blunt eye trauma are involved in traumatic high myopia [5].

Psychiatry related information on Lens, Crystalline

• The method might be useful for the study of aging of proteins such as eye lens and amyloid proteins derived from Alzheimer's disease [6].

High impact information on Lens, Crystalline

• These allelic cataract mutations provide the first direct evidence that MIP plays a crucial role in the development of a transparent eye lens [7].
• The murine Elo (eye lens obsolescence) mutation confers a dominant phenotype characterized by malformation of the eye lens [8].
• The eye lens contains a structural protein (alpha-crystallin), composed of two homologous primary gene products, alpha A2 and alpha B2 [9].
• We now show that protein S has a striking homology with the beta- and gamma-crystallins of the vertebrate eye lens which are beta-sheet proteins with internally duplicated structures [10].
• The three-dimensional structure of the eye lens protein, bovine gamma-crystallin II, has been determined at 2.6 A resolution [11].

Chemical compound and disease context of Lens, Crystalline

• Comparison of the amino acid sequence of the R. prowazekii PepA with the characterized leucine aminopeptidases from E. coli, Arabidopsis thaliana, and bovine eye lens revealed that 39.8, 34.9, and 34.0% of the residues were identical, respectively [12].
• These sulfonylureas are more potent osmotic agents than their predecessors and may give rise to crystalline lens changes and refractive error shifts in the absence of blood glucose variation [13].

Biological context of Lens, Crystalline

• The molecular structure and stability of the eye lens: x-ray analysis of gamma-crystallin II [11].
• The vimentin promoter drives expression of the MDR3 transgene in mesenchymal tissues and in the eye lens [14].
• The amino acid sequence of bovine eye lens leucine aminopeptidase has been determined [15].
• Oxidative stress targeted gamma-cystathionase in the eye lens upon aging, since the enzyme contained more carbonyl groups in old lenses than in young ones. gamma-Cystathionase mRNA was also markedly reduced in old lenses, thus contributing to the age-associated deficiency in gamma-cystathionase [16].
• A novel ENU-induced mutation in the mouse leading to a nuclear and zonular opacity of the eye lens (Aey1) was mapped to chromosome 1 between the markers D1Mit303 and D1Mit332 [17].

Anatomical context of Lens, Crystalline

• Here we characterize a large fragment of chicken gizzard and pig stomach desmin as well as the corresponding fragment from porcine eye lens vimentin [18].
• Expression of the MDR3 transgene was found in mesenchymal tissues, peripheral nerves, and the eye lens [19].
• The cells of the eye lens contain the type III intermediate filament protein vimentin, as well as two other intermediate filament proteins, CP49 and filensin [20].
• Effects of naphthalene metabolites on cultured cells from eye lens [21].
• The interactions observed in the loosely packed 3D crystals reported here may in fact represent an in vivo association mode between AQP0 tetramers from juxtaposed membranes in the eye lens [22].

Associations of Lens, Crystalline with chemical compounds

• Multiple interspecies differences were detected between humans and seven other mammals in 15 of the 24 metabolites measured in the intact crystalline lens and lens perchloric acid extracts [23].
• The reductone ascorbic acid, present in the crystalline lens in concentrations higher than those of glucose, is capable of undergoing nonenzymatic "browning" in the presence of lenticular proteins [24].
• We have studied the effect of polyethylene glycol (PEG) on the liquid-liquid phase separation (LLPS) of aqueous solutions of bovine gammaD-crystallin (gammaD), a protein in the eye lens [25].
• Enzyme/crystallins and extremely high pyridine nucleotide levels in the eye lens [26].
• The primary structure of leucine aminopeptidase from bovine eye lens [15].

Gene context of Lens, Crystalline

• For AR two primers (derived from the sequence of the rat eye lens) were chosen which amplify a 668-bp product [27].
• Of importance, the level of cyclin D1 was also elevated in eye lens of the c-mos-transgenic mice compared to normal mice [28].
• Gamma-crystallin genes are specifically expressed in the eye lens [29].
• The sequence relationship between the small heat shock proteins and the eye lens protein alpha-crystallin (Ingolia, T. D., and E. E. Craig, 1982, Proc. Natl. Acad. Sci. USA, 79: 2360-2364) prompted us to subject rat lenses in organ culture to heat shock and other forms of stress [30].
• alpha A-crystallin (alpha A) and alpha B-crystallin (alpha B) are among the predominant proteins of the vertebrate eye lens [31].

Analytical, diagnostic and therapeutic context of Lens, Crystalline

• By radioimmunoassay and immunofluorescence with antibodies specific for ACTH, we have detected these peptides in many areas of mouse embryos including neural tube, limb buds, eye lens, and myotomal muscles [32].
• Spectrophotometric measurements indicated that polymethyl methacrylate intraocular lenses do not mimic the crystalline lens in absorption of shortwave visible light and near-UV light [33].
• Organ culture and immunohistochemistry of the genetically malformed lens, in eye lens obsolescence, Elo, of the mouse [34].
• Electrophoretic titration curve in 6 M urea of the bovine eye lens protein alpha-crystallin [35].
• SETTING: Department of Ophthalmology, Dokkyo University School of Medicine, Tochigi, Japan. METHOD: Eight 10-week-old albino rabbits were prepared and anesthetized for phacoemulsification and aspiration of the crystalline lens and implantation of an acrylic or a hydrogel IOL [36].

References