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Clns1a  -  chloride channel, nucleotide-sensitive, 1A

Rattus norvegicus

Synonyms: Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, Clci, Clcni, I(Cln), ...
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Disease relevance of Clns1a

  • We demonstrated previously that expression of rat pICln in Escherichia coli conferred a strong resistance to hypotonic stress [1].
  • Fractionation of C6 glioma cells into plasma membrane- and cytoplasm-containing fractions demonstrated that approximately 90% of the recovered pICln was confined to the cytosol [2].

High impact information on Clns1a

  • Expression in Xenopus oocytes of a novel protein, pICln, activated a chloride conductance [3].
  • Monoclonal antibodies recognizing pICln blocked activation of a native hypotonicity-induced chloride conductance (ICl.swell) in Xenopus oocytes, suggesting that pICln may link actin-bound cytoskeletal elements to an unidentified volume-sensitive chloride channel [3].
  • The interaction between this protein and pICln was verified several ways, including the extraction of several pICln clones from a cDNA library using the 72-kDa protein as a bait in a yeast two-hybrid screen [4].
  • When added to the external medium bathing Sf9 cells, pICln inserted into the plasma membrane and increased cell cation permeability [5].
  • Phosphorylation of pICln with casein kinase II or mutation of G54, G56, and G58 to alanine decreased channel open probability and 86Rb+ uptake [5].

Chemical compound and disease context of Clns1a


Biological context of Clns1a

  • Mutagenesis studies led to the proposal that pICln is a swelling-activated anion channel [2].
  • Similarly, transfection of cells with a green fluorescent protein-labeled pICln construct failed to reveal any membrane localization of the protein [2].
  • pICln is found ubiquitously in mammalian cells and is postulated to play a critical role in cell volume regulation [2].
  • The pICln-associated kinase displayed broad substrate specificity and was inhibited in a concentration-dependent manner by heparin, zinc and 5,6-dichloro-1-beta-D-ribofuranosylbenose (DRB) [6].

Anatomical context of Clns1a

  • We tested the anion channel hypothesis by reconstituting recombinant pICln into artificial and biological membranes [5].
  • We report the cloning and sequencing from human reticulocytes of cDNA coding for the Cl- channel-associated protein, pICln [7].
  • Human reticulocyte pICln (HRpICln) cDNA encodes a protein (predicted molecular mass 26293Da) identical with human non-pigmented ciliary epithelial cell pICln [7].
  • Moreover the association of RBC pICln with actin offers a model in which to test interactions between RBC ion channels and the cytoskeleton [7].
  • Human NPE cell lines exhibited significant levels of pICln transcripts [8].

Associations of Clns1a with chemical compounds

  • Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln [3].
  • Phosphopeptide mapping demonstrated that pICln contains at least two phosphorylated serine residues that are located on trypsin cleavage fragments rich in acidic amino acid residues [6].

Other interactions of Clns1a

  • This study investigates changes in the messenger RNA (mRNA) expression levels of HCN2 and HCN4 encoding rat If channels; ClC-3, a candidate gene for swelling-activated Cl- channel, and pICln, a regulatory subunit of Cl- channels in rat hypertrophied heart induced by banding the abdominal aorta [9].

Analytical, diagnostic and therapeutic context of Clns1a


  1. A peptide derived from pICln induced a strong hypotonic resistance in Escherichia coli cells. Tao, G.Z., Tashima, Y. Peptides (2000) [Pubmed]
  2. Effect of cell swelling on membrane and cytoplasmic distribution of pICln. Emma, F., Breton, S., Morrison, R., Wright, S., Strange, K. Am. J. Physiol. (1998) [Pubmed]
  3. Molecular characterization of a swelling-induced chloride conductance regulatory protein, pICln. Krapivinsky, G.B., Ackerman, M.J., Gordon, E.A., Krapivinsky, L.D., Clapham, D.E. Cell (1994) [Pubmed]
  4. pICln binds to a mammalian homolog of a yeast protein involved in regulation of cell morphology. Krapivinsky, G., Pu, W., Wickman, K., Krapivinsky, L., Clapham, D.E. J. Biol. Chem. (1998) [Pubmed]
  5. Recombinant pICln forms highly cation-selective channels when reconstituted into artificial and biological membranes. Li, C., Breton, S., Morrison, R., Cannon, C.L., Emma, F., Sanchez-Olea, R., Bear, C., Strange, K. J. Gen. Physiol. (1998) [Pubmed]
  6. Characterization of pICln phosphorylation state and a pICln-associated protein kinase. Sanchez-Olea, R., Emma, F., Coghlan, M., Strange, K. Biochim. Biophys. Acta (1998) [Pubmed]
  7. Molecular cloning and expression of a chloride channel-associated protein pICln in human young red blood cells: association with actin. Schwartz, R.S., Rybicki, A.C., Nagel, R.L. Biochem. J. (1997) [Pubmed]
  8. Molecular cloning of the human volume-sensitive chloride conductance regulatory protein, pICln, from ocular ciliary epithelium. Anguita, J., Chalfant, M.L., Civan, M.M., Coca-Prados, M. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
  9. Ion channel remodeling in cardiac hypertrophy is prevented by blood pressure reduction without affecting heart weight increase in rats with abdominal aortic banding. Hiramatsu, M., Furukawa, T., Sawanobori, T., Hiraoka, M. J. Cardiovasc. Pharmacol. (2002) [Pubmed]
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